Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Tuesday 9th August 1.1 Lab work 1.1.1 Visualization 1.1.1.1 Heat shock transformation Tuesday 9th August Lab work Visualization Heat shock transformation By Charlène and Terrence For 1.2, 4.2, SgNm, SgSt1, Detection, FRB and FKBP gBlocks, the results of the sequencing were not as expected, so we transformed them a second time (in puc19). We followed the usual protocol. 50µL of cells were streaked on LB + Amp (50µg/mL) + IPTG + xGal.
By Charlène and Terrence
For 1.2, 4.2, SgNm, SgSt1, Detection, FRB and FKBP gBlocks, the results of the sequencing were not as expected, so we transformed them a second time (in puc19). We followed the usual protocol. 50µL of cells were streaked on LB + Amp (50µg/mL) + IPTG + xGal.
