Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Monday 8st August 1.1 Lab work 1.1.1 Visualization 1.1.1.1 Phusion PCR on pPS16_006 and pPS16_007 1.1.1.2 Liquid cultures of bacteria containing plasmid to extract 1.1.1.3 Migration of DNA plasmid extracted Monday 8st August Lab work Visualization Phusion PCR on pPS16_006 and pPS16_007 By Caroline The PCR was carried out following the usual protocol adapted to 50µL and with a TM at 72°C. The specific primers for each parts were using. The products were put to migrated on a 0.8%agarose gel with BET. No amplification were observed that was probably due to the fact that the extractions made on the 3/08/2016 were eluted with water which is not working with this extraction kit. Liquid cultures of bacteria containing plasmid to extract By Caroline, Charlène, Terrence In order to redo the extractions that did not working due to the use of water instead of the elution buffer. To do it, 5mL of LB were mixed with Ampicillin at 50µg/mL at put at 37°C and at 180rpm overnight. Migration of DNA plasmid extracted By Terrence, Alice, Laetitia File:T--Paris_Saclay--160728_visualization_migration_extraction.jpeg
By Caroline
The PCR was carried out following the usual protocol adapted to 50µL and with a TM at 72°C. The specific primers for each parts were using. The products were put to migrated on a 0.8%agarose gel with BET. No amplification were observed that was probably due to the fact that the extractions made on the 3/08/2016 were eluted with water which is not working with this extraction kit.
By Caroline, Charlène, Terrence
In order to redo the extractions that did not working due to the use of water instead of the elution buffer. To do it, 5mL of LB were mixed with Ampicillin at 50µg/mL at put at 37°C and at 180rpm overnight.
By Terrence, Alice, Laetitia
File:T--Paris_Saclay--160728_visualization_migration_extraction.jpeg
