Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Thursday 11th August 1.1 Lab work 1.1.1 Visualization 1.1.1.1 Transformation of DH5a cells with pPS16_009 1.1.1.2 pPS16_010 extraction 1.1.1.3 DreamTaq PCR of transformed cell with pPS16_002,008,010, 011, 012, 013, 014 and puC 19 Thursday 11th August Lab work Visualization Transformation of DH5a cells with pPS16_009 By Léa Dh5a cells were transformed with pPS16_009, or psB1c3 (control), or not transformed (control)using the usual protocol. pPS16_010 extraction By Alice After screening colony screening PCR, 3 clones (clones 3, 7 and 8) transformed with pPS16_010 seemed to have the insert of interest. Plasmids from these clones were extracted following this protocol. After extraction, plasmids were migrated on a gel.z Migration of FRB plasmid DreamTaq PCR of transformed cell with pPS16_002,008,010, 011, 012, 013, 014 and puC 19 By Léa and Laetitia PCR DreamTaq was performed on 6 clones of each transformed celles from 10/08/16. Each clone was plated and put in liquid culture with LB and Ampicillin.
By Léa
Dh5a cells were transformed with pPS16_009, or psB1c3 (control), or not transformed (control)using the usual protocol.
By Alice
After screening colony screening PCR, 3 clones (clones 3, 7 and 8) transformed with pPS16_010 seemed to have the insert of interest. Plasmids from these clones were extracted following this protocol. After extraction, plasmids were migrated on a gel.z
By Léa and Laetitia
PCR DreamTaq was performed on 6 clones of each transformed celles from 10/08/16. Each clone was plated and put in liquid culture with LB and Ampicillin.
