Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Friday 12th August 1.1 Lab work 1.1.1 Visualization 1.1.1.1 PCR of pPS16_009 1.1.1.2 Extraction of puc19 Friday 12th August Lab work Visualization PCR of pPS16_009 By Léa A Dreamtaq PCR was performed on pPS16_009 using the usual protocol. Primers: IPS83 ans IPS84 Extraction of puc19 By Charlène Clones 1 and 2 of puc19 were extracted with the Plasmid MiniPrep kit. They were fast digested with EcoRI : 3µL of plasmid + 1µL of FD buffer + 0.5µL of FD EcoRI + 5.5µL of water, 15 minutes at 37°C.
By Léa
A Dreamtaq PCR was performed on pPS16_009 using the usual protocol. Primers: IPS83 ans IPS84
By Charlène
Clones 1 and 2 of puc19 were extracted with the Plasmid MiniPrep kit.
They were fast digested with EcoRI : 3µL of plasmid + 1µL of FD buffer + 0.5µL of FD EcoRI + 5.5µL of water, 15 minutes at 37°C.
