Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Tuesday 16th August 1.1 Lab work 1.1.1 Visualization 1.1.1.1 2.1-2.2 and 3.1-3.2 ligation 1.1.1.2 Q5 PCR on the ligation products and pPS16_008 clones 1 and 2 Tuesday 16th August Lab work Visualization 2.1-2.2 and 3.1-3.2 ligation By Charlène 8µL of 2.1 purify PCR products, 8µL of 2.2 purify PCR products, 2µL of ligase T4 Buffer and 2L of ligase T4 were mixed. 8µL of 3.1 purify PCR products, 8µL of 3.2 purify PCR products, 2µL of ligase T4 Buffer and 2µL of ligase T4 were mixed. They were incubated for 1h at RT. Q5 PCR on the ligation products and pPS16_008 clones 1 and 2 By Charlène The PCR was carried out with a new protocol : The specific primers for each parts were using. The products were put to migrated on a 0.8%agarose gel with BET.
By Charlène
8µL of 2.1 purify PCR products, 8µL of 2.2 purify PCR products, 2µL of ligase T4 Buffer and 2L of ligase T4 were mixed. 8µL of 3.1 purify PCR products, 8µL of 3.2 purify PCR products, 2µL of ligase T4 Buffer and 2µL of ligase T4 were mixed. They were incubated for 1h at RT.
The PCR was carried out with a new protocol :
The specific primers for each parts were using. The products were put to migrated on a 0.8%agarose gel with BET.
