Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Monday 1st August 1.1 Lab work 1.1.1 Visualization 1.1.1.1 New gBlocks spacer 1, Nm_sgRNA, ATG_linker-FKBP, St_sgRNA, Detection, ATG_linker-RFB insertion in puc19 1.1.1.2 Transformation of new gBlocks and 1.2, 1.2, 3.1 and 4.1 inserted in puc19 1.1.1.3 gBlock 3.1 insertion in puc19 1.1.1.4 DH5alp|pPS16_003, DH5alp|pPS16_004, DH5alp|pPS16_006 and DH5alp|pPS16_007 streak to recover colonies Monday 1st August Lab work Visualization New gBlocks spacer 1, Nm_sgRNA, ATG_linker-FKBP, St_sgRNA, Detection, ATG_linker-RFB insertion in puc19 By Léa and Charlène The insertion of these gblocks was carried out following the usual protocol. Transformation of new gBlocks and 1.2, 1.2, 3.1 and 4.1 inserted in puc19 By Léa and Mathilde gBlocks spacer 1, Nm_sgRNA, ATG_linker-FKBP, St_sgRNA, Detection, ATG_linker-RFB,1.2, 1.2, 3.1 and 4.1 inserted in puc19 were transformed following the usual protocol. For each plasmid we streaked 50µL and 150µL of bacteria on LB + X-Gal + IPTG Petri dishes. gBlock 3.1 insertion in puc19 By Caroline The insertion was carried out following the usual protocol. DH5alp|pPS16_003, DH5alp|pPS16_004, DH5alp|pPS16_006 and DH5alp|pPS16_007 streak to recover colonies By Caroline Checked DH5alp transformed with pPS16_003, pPS16_004, pPS16_006 and pPS16_007 were streaked on Pteri dishes containing LB + Amp (50µg/mL) + IPTG (0.1 µL/mL) + Xgal (0.25µL/mL) from glycerol cultures.
By Léa and Charlène
The insertion of these gblocks was carried out following the usual protocol.
By Léa and Mathilde
gBlocks spacer 1, Nm_sgRNA, ATG_linker-FKBP, St_sgRNA, Detection, ATG_linker-RFB,1.2, 1.2, 3.1 and 4.1 inserted in puc19 were transformed following the usual protocol. For each plasmid we streaked 50µL and 150µL of bacteria on LB + X-Gal + IPTG Petri dishes.
By Caroline
The insertion was carried out following the usual protocol.
Checked DH5alp transformed with pPS16_003, pPS16_004, pPS16_006 and pPS16_007 were streaked on Pteri dishes containing LB + Amp (50µg/mL) + IPTG (0.1 µL/mL) + Xgal (0.25µL/mL) from glycerol cultures.
