Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Thursday 4st August 1.1 Lab work 1.1.1 Visualization 1.1.1.1 DNA Extraction of pPS16_001, pPS16_002, pPS16_003, pPS16_009,DS-NMcasN-, DS-TDcasN-, DS-SPcasN- 1.1.1.2 Stock of glycerol for pPS16_001, pPS16_002, pPS16_003, pPS16_009 and DS-NMcasN- 1.1.1.3 PCR Clean-up with the NucleoSpin kit Thursday 4st August Lab work Visualization DNA Extraction of pPS16_001, pPS16_002, pPS16_003, pPS16_009,DS-NMcasN-, DS-TDcasN-, DS-SPcasN- By Caroline, Naiane, Mathilde and Laetitia Extraction was done on: Puc 19 containing Clone pPS16_001 2, 9 pPS16_002 4, 7, 11 pPS16_009 7 pPS16_003 4 DS-NMcasN- 7, 11 DS-TDcasN- 1 DS-SPcasN- 1 The extraction was performed using the kit. We followed this protocol. Stock of glycerol for pPS16_001, pPS16_002, pPS16_003, pPS16_009 and DS-NMcasN- By Naiane, Mathilde and Laetitia Stocks of glycerol were made with: 700 µL of culture 300 µL of glycerol (60%) PCR Clean-up with the NucleoSpin kit By Caroline The purification was carried out on DH5alp|pPS16_003, DH5alp|pPS16_004 and DH5alp|pPS16_007 following the usual protocol. But, I forgot to dilute the sample with the Buffer NT3 with 100mL ethanol befor I began the purification and so no DNA was present on the 0.8% agarose gel.
By Caroline, Naiane, Mathilde and Laetitia
Extraction was done on:
The extraction was performed using the kit. We followed this protocol.
By Naiane, Mathilde and Laetitia
Stocks of glycerol were made with:
By Caroline
The purification was carried out on DH5alp|pPS16_003, DH5alp|pPS16_004 and DH5alp|pPS16_007 following the usual protocol. But, I forgot to dilute the sample with the Buffer NT3 with 100mL ethanol befor I began the purification and so no DNA was present on the 0.8% agarose gel.
