Team:USP UNIFESP-Brazil

MENU ▤

Welcome to iGEM 2016!

Team Description

We are a multidisciplinary team from São Paulo, Brasil, with students of architecture, biology, biomedical sciences, social sciences and more,from the universities USP, UNESP and UNIFESP. The team originated from the synthetic biology club (SynBio Brasil), which is a independent club that works promoting synbio and open science awereness and education. Since 2012, different members of the club have organized themselves to take part in iGEM competition .

This year, our project is based on the heterologous expression of spider silk protein in Chlamydomonas reinhardtii . The project started when we looked at the problem of growing antibiotic resistance and started to think in ways to tackle it. We specially focused on injury related infections, for example in the case of burn victms. We devised the creation of an antibiotic patch, combining the physical properties of spider silk with antibiotic enzymes (enzybiotics). We intend to express both the spider silk and chimeric enzybiotic proteins with spider silk motifs in Chlamydomonas and polymerize then together to form the product of interest. We hope to acomplish, besides the final goal of patch development, improvement of Chlamydomonas as a synbio chassis and analysis of its capability of producing enzybiotics and monomers of spider silk. Moreover, the team is envolved with open hardware developement and promotion and synthetic biology popularization, helping to promote the synthetic biology culture in Brazil, raising awareness and engaging the public.

Help the team!

Brazilian campaign

International campaign



August
                                 
                                 
                                 
                               </a>
                               </h4>
                           </div>

2nd August 2016

Tessa

Amplified E0840 (GFP) out of a pSB1C3-GFP plasmid using Phusion PCR. Four reactions of 50µl.

Nanodropped PCR products.

Nanodrop

<thead> 
                                               </thead>
                                               <tbody>
</tbody>
Product Concentration (ng/µl)
E0840 339.6
E0840 554.1
E0840 596.9
E0840 567.6

Ran a 1% agarose gel of the PCR product.

                                           <img src="T--TU_Delft--Electrophoresis_20160802.png" alt="electrophoresis">

Stored product 2 and 4 in the fridge for later use.

10th August 2016

Tessa

Restricted PCR product of 3rd August, J23100 E0840, J23113 E0840 and J23117 E0840, with EcoRI-HF and PstI.

Purified restriction product.

Nanodropped purified product.

Nanodrop

<thead> 
                                               </thead>
                                               <tbody>
</tbody>
Product Concentration (ng/µl)
J23100 E0840 14.7
J23113 E0840 21.3
J23117 E0840 23.3