Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Tuesday 2st August 1.1 Lab work 1.1.1 Visualization 1.1.1.1 DreamTaq PCR on DH5alp|pPS16_001, DH5alp|pPS16_002, DH5alp|pPS16_005, DH5alp|pPS16_009, DH5alp|pPS16_0010, DH5alp|pPS16_011, DH5alp|pPS16_012, DH5alp|pPS16_013, DH5alp|pPS16_014, DH5alp|pPS16_015 1.1.1.2 Sequencing of the gBlock 4.2 1.1.1.3 Culture of DH5alp|pPS16_003, DH5alp|pPS16_004, DH5alp|pPS16_006, DH5alp|pPS16_007 and DH5alp|pPS16_008 1.1.2 Interlab Study 1.1.2.1 Device 1 transformation Tuesday 2st August Lab work Visualization DreamTaq PCR on DH5alp|pPS16_001, DH5alp|pPS16_002, DH5alp|pPS16_005, DH5alp|pPS16_009, DH5alp|pPS16_0010, DH5alp|pPS16_011, DH5alp|pPS16_012, DH5alp|pPS16_013, DH5alp|pPS16_014, DH5alp|pPS16_015 By Charlène, Mathilde, Laetitia, Caroline and Léa A Low fidelity PCR was carried out following the usual protocol and using the universal puc19 primers. 6 clones for each transformations were tested. Each clone was re-plated and put at 37°C. We also did a liquid culture for each clone in 3mL of LB and Ampicilin(37°C and 180 rpm). Results Results Results Sequencing of the gBlock 4.2 By Caroline The PCR products obtained with Q5 on pPS16_008 were sent for sequencing. Culture of DH5alp|pPS16_003, DH5alp|pPS16_004, DH5alp|pPS16_006, DH5alp|pPS16_007 and DH5alp|pPS16_008 By Caroline One colonie from each transformation was put into LB + Amp (50µg/mL) and incubated overnight at 37°C and 180rpm. Interlab Study Device 1 transformation By Caroline The device 1 was transformed in DH5alp following the usual Heat-shock protocol. The transformations were streaked on Petri dishes LB+Amp(50µg/mL)+IPTG(0.1µL/mL)+XGal(0.25µL/mL) and incubated overnight at 37°C.
By Charlène, Mathilde, Laetitia, Caroline and Léa
A Low fidelity PCR was carried out following the usual protocol and using the universal puc19 primers. 6 clones for each transformations were tested.
Each clone was re-plated and put at 37°C. We also did a liquid culture for each clone in 3mL of LB and Ampicilin(37°C and 180 rpm).
By Caroline
The PCR products obtained with Q5 on pPS16_008 were sent for sequencing.
One colonie from each transformation was put into LB + Amp (50µg/mL) and incubated overnight at 37°C and 180rpm.
The device 1 was transformed in DH5alp following the usual Heat-shock protocol. The transformations were streaked on Petri dishes LB+Amp(50µg/mL)+IPTG(0.1µL/mL)+XGal(0.25µL/mL) and incubated overnight at 37°C.
