Team:Paris Saclay/Notebook/August/22

Monday 22th August

Lab work

Visualization

Low fidelity Dreamn Taq PCR of pPS16_016

By Mathilde

Clones 7,8,9 and 10 of pPS16_016 were amplified following the protocol :

  • 1,5µL of Green Buffer
  • 1µL of dNTPs
  • 1µL of each primers 1151 and 1151
  • 0,13µL of Dream Taq Polymerase
  • 19,37 µL of H20

Colonies were put into sterile water, and were put trhough