Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Wednesday 24th August 1.1 Lab work 1.1.1 Visualization 1.1.1.1 Migration gel of Gibson (fragment 3 + fragment 4) and GFP in PSB1C3 1.1.1.2 pPS16_002 (1.2) clones 8 and 14 and pPS16_014(SgRna Nm) clone 12 Extraction Wednesday 24th August Lab work Visualization Migration gel of Gibson (fragment 3 + fragment 4) and GFP in PSB1C3 By Terrence Clone 6 and 8 seems correct for the Gibson. Clone 2-4-7-11 are correct. pPS16_002 (1.2) clones 8 and 14 and pPS16_014(SgRna Nm) clone 12 Extraction By Terrence The extraction was carried out following the usual protocol. Result of the extraction Nano drop : size (ng/µL) 260/230 260/280 1.2.14 47.81 1.64 1.84 1.2.8 834.5 2.40 1.99 KCl 250mM SgRna Nm 12 124.21 2.00 1.89
By Terrence
The extraction was carried out following the usual protocol.
Nano drop :
