Difference between revisions of "Team:SYSU-Software/Project"

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                         <div id = "overview_P">
 
                         <div id = "overview_P">
 
                             <ul>
 
                             <ul>
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                                 <li><p>We transformed Escherichia coli DH5α with Device 1, 2, 3, positive control and negative control. Then we </p></li>
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                                 <li><p>CRFAT (Community-based Retro-synthetic Analysis Functional plaTform) is a integrated software for crafting synthetic</p></li>
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                                 <li><p>inoculated them on LB plate which containing chloramphenicol. Then, we picked single colonies, afterwards, </p></li>
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                                <li><p>biological system from design, validation to share. CRAFT has three functional parts, CRAFT Designer, CRAFT</p></li>
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                                 <li><p>moved them into liquid cultures (LB medium). After an overnight cultivation, liquid cultures were diluted </p></li>
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                                <li><p>Square and Satellite.</p></li>
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                                 <li><p>with LB medium until the value of OD600 reached 0.02. Then we took out 100 μL samples from the cultures </p></li>
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                                 <li><p>CRAFT Designer follows the concept of evolution, in which users can customize their synthetic biological system</p></li>
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                                 <li><p>every hour (until 6 hours). At last, we measured OD600 and fluorescence of all samples.</p></li>
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                                 <li><p>based on their restrictions including the background environment, simulating natural selection pressure. CRAFT Designer</p></li>
 +
                                 <li><p>also provides users with standard protocol. It refers to the concept of “Design and Redesign” in synthetic biology</p></li>
 +
                                 <li><p>and applies it in protocol design: fill the standard protocol frames with messages corresponding to user designed systems.</p></li>
 +
                                <li><p>CRAFT Square is a communication platform, in which users can share and exchange their ideas, or modify other designs</p></li>
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                                <li><p>to suit their own demands. Satellite serves as the distributed computing plug-in in CRAFT, undertaking nearly all calculation</p></li>
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                                <li><p> in the whole software.</p></li>
 
                             </ul>
 
                             </ul>
 
                         </div>
 
                         </div>

Revision as of 08:34, 18 October 2016

SYSU-Software:Project

OVERVIEW

  • CRFAT (Community-based Retro-synthetic Analysis Functional plaTform) is a integrated software for crafting synthetic

  • biological system from design, validation to share. CRAFT has three functional parts, CRAFT Designer, CRAFT

  • Square and Satellite.

  • CRAFT Designer follows the concept of evolution, in which users can customize their synthetic biological system

  • based on their restrictions including the background environment, simulating natural selection pressure. CRAFT Designer

  • also provides users with standard protocol. It refers to the concept of “Design and Redesign” in synthetic biology

  • and applies it in protocol design: fill the standard protocol frames with messages corresponding to user designed systems.

  • CRAFT Square is a communication platform, in which users can share and exchange their ideas, or modify other designs

  • to suit their own demands. Satellite serves as the distributed computing plug-in in CRAFT, undertaking nearly all calculation

  • in the whole software.