Difference between revisions of "Team:Michigan/Results"

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   <div class = "container"> <h1 style="text-align:center; font-size: 75px;"><font face= "Poiret One">Results</font></h1>
 
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          <p style="text-align:center; font-size:20px;"><font face="verdana"> In order to make the most meaningful impact, we sought the opinions of experts in the field of synthetic biology and tuberculosis treatment and integrated their advice into the design of our diagnostic test. Numerous experts with real life experience in tuberculosis diagnosis and care guided us through the process of designing an ideal diagnostic test. This, together with our literature research and ideas, helped us design Aptapaper so that it would actually be useful if made into a real product. Based on the weaknesses of competing diagnostic technologies and the encouragement of our advisors, we believe that our tool would be heavily adopted. </font></p>
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<h1 style="text-align:center; font-size: 50px;"><font face= "Poiret One">Proximity Ligation Assay in agarose gel using sybr</h2>  
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<p style="text-align:center; font-size:20px;"><font face="verdana"> <p>CAPTION HERE </font><br><hr></p> <img src="https://static.igem.org/mediawiki/2016/8/8c/TestingPLAagarose.JPG" width="500" height="500" style="float:center"></div>
1) Rehydrated DNA from distribution kit
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2) Transformed into DH5a chemically competent cells
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3) Extracted plasmid
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4) PCR mutagenesis to engineer desired cut sites around LacZ
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          <h1 style="text-align:center; font-size: 50px;"><font face= "Poiret One">Proximity Ligation Assay in an agarose gel using sybr </h2>
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                  <p style="text-align:center; font-size:20px;"><font face="verdana"> *To read more on what we talked about with Mr. Kannenje, please visit our <a href = "https://2016.igem.org/Team:Michigan/factsheet" target="_blank"><u>Bench-to-Bedside Guide</u></a>, and click on the "Kenya Case Study" tile, found under Market Analysis, II. Target Market, Market Need, & Customer Discovery.
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        <h1 style="text-align:center; font-size: 50px;"> <font face= "Poiret One">Creation of delta-M15 mutant</h2>
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2) Submitted for sequencing to confirm successful deletion
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          <h1 style="text-align:center; font-size: 50px;"><font face= "Poiret One">Proximity dependent ligation assay </h2>
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        <p style="text-align:center; font-size:20px;"><font face="verdana"> 1) Probe segments and bridge segments mixed with T4 ligase in presence or absence of thrombin
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2) Gel electropheresis to detect ligation
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         <h1 style="text-align:center; font-size: 50px;"> <font face= "Poiret One">Cloned delta-M15 mutant into submission vector
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         <h1 style="text-align:center; font-size: 50px;"> <font face= "Poiret One">Acknowledgements</h2>
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         <p style="text-align:center; font-size:20px;"><font face="verdana"> 1) Digested linearized pSB1C3 with our mutant
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<p style="text-align:center; font-size:20px;"><font face="verdana">We would like to sincerely thank Rama Kannenje and Grace Hsia for their helpful advice and time as well as all of the people who collaborated with their help and opinions to our project.</p>
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2) Ligated together
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<p style="text-align:center; font-size:20px;"><font face="verdana"> 3) Transformed into new DH5a chemically competent cells
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5) Extracted DNA
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Revision as of 00:07, 20 October 2016

Results

In order to make the most meaningful impact, we sought the opinions of experts in the field of synthetic biology and tuberculosis treatment and integrated their advice into the design of our diagnostic test. Numerous experts with real life experience in tuberculosis diagnosis and care guided us through the process of designing an ideal diagnostic test. This, together with our literature research and ideas, helped us design Aptapaper so that it would actually be useful if made into a real product. Based on the weaknesses of competing diagnostic technologies and the encouragement of our advisors, we believe that our tool would be heavily adopted.

Proximity Ligation Assay in agarose gel using sybr

CAPTION HERE


*To read more on what we talked about with Mr. Kannenje, please visit our Bench-to-Bedside Guide, and click on the "Kenya Case Study" tile, found under Market Analysis, II. Target Market, Market Need, & Customer Discovery.

Acknowledgements


We would like to sincerely thank Rama Kannenje and Grace Hsia for their helpful advice and time as well as all of the people who collaborated with their help and opinions to our project.