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<b>Day 10:</b> We serially diluted and spot plated the GPM plates with Serratia Marcescens and Top 10 E. coli, then incubated for 24 hours .
 
<b>Day 10:</b> We serially diluted and spot plated the GPM plates with Serratia Marcescens and Top 10 E. coli, then incubated for 24 hours .
  
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<b>Day 11:</b> After 24 hours of incubation at 37 Celsius we were able to see growth for both bacteria and set the plates out to incubate another 24 hours at room temperature.
 
<b>Day 11:</b> After 24 hours of incubation at 37 Celsius we were able to see growth for both bacteria and set the plates out to incubate another 24 hours at room temperature.
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Revision as of 01:56, 20 October 2016





Collaborations

Sharing and collaboration are core values of iGEM. We encourage you to reach out and work with other teams on difficult problems that you can more easily solve together.

University of Texas at Austin

University of Texas at Austin - Texas Tech S. paucimobilis Plate Experience:

Listed below is a brief write up concluding the experiences of the Texas Tech iGEM team testing a protocol for making gelatin plates to be used for growing bacteria instead of agar plates in collaboration with the UT iGEM team.

Day 1: Upon receiving a streak plate of Sphingamonas Paucimobilis from the UT iGEM team, we incubated the plate at room temperature for two days.

Day 3: After two days of incubation a single colony was picked and used to inoculate 20mL of YM Broth, then incubated for 24 hours at 30 degrees Celsius.

YM Broth (g/L)
  • Yeast Extract: 3.0g
  • Dextrose: 10.0g
  • Peptone: 5.0g
  • Malt Extract: 3.0g

Day 4: After 24 hours we inoculated a 180ml culture of Gellan Gum Production Media (GPM) with the 20mL YM culture from day 3. After inoculating the GPM was incubated at 30 degrees for 6 days.

Gellan Gum Production Media (g/L)

  • Dextrose: 20.0g
  • Na2HPO4: 10.0g
  • K2SO4: 1.0g
  • NaCl: 1.0g
  • (NH4)2SO4: 0.15g
  • CaCl2-2H2O: 0.01g
  • MgSO4-7H2O: 0.2g
  • FeSO4-7H2O: 0.001g
  • Yeast extract: 0.5 (pH to 6.8-7.0 w/ HCl)



Day 10: We serially diluted and spot plated the GPM plates with Serratia Marcescens and Top 10 E. coli, then incubated for 24 hours .



Day 11: After 24 hours of incubation at 37 Celsius we were able to see growth for both bacteria and set the plates out to incubate another 24 hours at room temperature.
Day 12:




Header

Here are some suggestions for projects you could work on with other teams:
  • Improve the function of another team's BioBrick Part or Device
  • Characterize another team's part
  • Debug a construct
  • Model or simulating another team's system
  • Test another team's software
  • Help build and test another team's hardware project
  • Mentor a high-school team

Day 9: After 6 days of incubation we autoclaved the GPM culture and added 8mL of sterilized 40x LB broth. Lastly, we thoroughly mixed the 2 solutions and immediately poured the GPM + LB solution into petri dishes. Overnights of Serratia Marcescens and Top 10 E. coli were made to inoculate the GPM plates with on day 10.