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<div id="Project_Description"><img src="https://static.igem.org/mediawiki/2016/5/58/T--HokkaidoU_Japan--proof_of_concept.png" width="300px" height="90px" alt="Proof of concept"></div> | <div id="Project_Description"><img src="https://static.igem.org/mediawiki/2016/5/58/T--HokkaidoU_Japan--proof_of_concept.png" width="300px" height="90px" alt="Proof of concept"></div> | ||
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After we finished cultivating samples, | After we finished cultivating samples, | ||
we took 100 µL out of each samples and made the following operation. | we took 100 µL out of each samples and made the following operation. |
Revision as of 12:58, 18 October 2016
Team:HokkaidoU Japan
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We conducted SDS-PAGE with (BBa_K2015012+BBa_K2015008/ BBa_K2015012+BBa_K2015009) on (pSB1C3)of E.coli(DH5α). At first, we made the Gel for SDS-PAGE, with the following the Table1. What was the most important was to add TEMED ffinally because
The following Table. 2 is about preparing the SDS-PAGE.
We conducted SDS-PAGE with (BBa_K2015012+BBa_K2015008/ BBa_K2015012+BBa_K2015009) on (pSB1C3)of E.coli(DH5α). At first, we made the Gel for SDS-PAGE, with the following the Table1. What was the most important was to add TEMED ffinally because
Table. 1. Gel assay |
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The following Table. 2 is about preparing the SDS-PAGE.
Table. 2. Experimental condition |
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After we finished cultivating samples, we took 100 µL out of each samples and made the following operation.
The fig. 1 shows the result of SDS-PAGE. |