Team:HokkaidoU Japan/Notebook

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Team:HokkaidoU Japan

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• Top
• Project
  • Overview
    • Project Description
    • Background
    • Proof of concept
    • Modeling
    • Achievements
  • MULTIMERIZATION
    • Overview
    • Methods
    • Results
    • Conclusion
  • FUTURE WORK
    • Circularization
    • Kill Switch
    • Transport
    • Aggregation
  • Extra Materials
    • Parts
    • Notebook
    • Protocols
    • Safety
• Human practices
• Collaborations
• Team
  • Attribution
  • Member

Protocol

August 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

September 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Octorber 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

2016/08/07

Transformation

Researcher: Fujita

Part: BBa_B0034 on pSB1A2, BBa_E0040 on pSB1A2 BBa_R0010 on pSB1C3, BBa_B0015 on pSB1C3, BBa_J04500 on pSB1C3, BBa_E0020 on pSB1C3, BBa_E0030 on pSB1C3

Liquid Culture

Researcher: Fujita, Kishida, Kono

Part: BBa_E0020 on pSB1C3, BBa_E0030 on pSB1C3, BBa_E0040 on pSB1A2, BBa_R0010 on pSB1C3

2016/08/09

Transformation

Researcher: Kono, Mimata, Nishimura

Part: BBa_B0034 on pSB1A2, BBa_E0040 on pSB1A2, BBa_R0010 on pSB1C3, BBa_B0015 on pSB1C3, BBa_J04500 on pSB1C3

Mini-prep

Researcher: Fujita, Kishida, Kono

Part: BBa_R0010 on pSB1A2, BBa_E0040 on pSB1A2

Gel Free System

Researcher: Mimata, Shibata, Kono, Nishimura

Part: BBa_R0010, BBa_E0040

Ethanol Precipitation

Researcher: Mimata, Shibata, Kono, Nishimura

Part: BBa_R0010, BBa_E0040

2016/08/10

Liquid Culture

Researcher: Mimata

Part: BBa_B0015 on pSB1C3, BBa_J04500 on pSB1C3, BBa_R0010 on pSB1C3

Transformation

Researcher: Mimata

Part: BBa_B0034 on pSB1A2, BBa_E0040 on pSB1A2

Electrophoresis

Researcher: Kishimoto, Kono, Shibata, Nishimura

Part: BBa_R0010, BBa_E0040

Mini-prep

Researcher: Mimata, Kono

Part: BBa_R0010 on pSB1A2, BBa_B0015 on pSB1C3, BBa_J04500 on pSB1C3

Electrophoresis

Researcher: Mimata, Kono, Nishimura

Part: BBa_R0010 on pSB1A2, BBa_B0015 on pSB1C3, BBa_J04500 on pSB1C3

Liquid Culture

Researcher: Kono, Mimata, Nishimura

Part: BBa_B0034 on pSB1A2, BBa_E0040 on pSB1C3

2016/08/11

Transformation

Researcher: Nishimura, Kishida

Part: BBa_B0032 on pSB1C3, BBa_R0011 on pSB1A2

Mini-prep

Researcher: Nishimura, Kishida

Part: BBa_E0040 on pSB1A2, BBa_B0034 on pSB1A2

PEG Precipitation

Researcher: Nishimura, Shibata, Noda

Part: BBa_B0015 on pSB1C3, BBa_R0010 on pSB1C3 , BBa_J04500 on pSB1C3, BBa_E0040 on pSB1A2, BBa_B0034 on pSB1A2

Electrophoresis

Researcher: Shibata, Noda

Part: BBa_B0015 on pSB1C3, BBa_R0010 on pSB1C3 , BBa_J04500 on pSB1C3, BBa_E0040 on pSB1A2, BBa_B0034 on pSB1A2

Gel Free System

Researcher: Kono, Nishimura

Part: BBa_B0015, BBa_R0010, BBa_J04500, BBa_E0040, BBa_B0034

Electrophoresis

Researcher: Nishimura

Part: BBa_B0015, BBa_R0010, BBa_J04500, BBa_E0040, BBa_B0034

Ethanol Precipitation

Researcher: Kono, Nishimura

Part: BBa_B0015, BBa_R0010, BBa_J04500, BBa_E0040, BBa_B0034

Electrophoresis

Researcher: Kono, Nishimura

Part: BBa_B0015, BBa_R0010, BBa_J04500, BBa_E0040, BBa_B0034

2016/08/12

Transformation

Researcher: Nishimura

Part: BBa_C0012 on pSB1C3, BBa_J23101 on BBa_J61002

Liquid Culture

Researcher: Nishimura

Part: BBa_B0032 on pSB1C3, BBa_R0011 on pSB1A2

2016/08/13

Mini-prep

Researcher: Fujita

Part: BBa_B0032 on pSB1C3

PEG Precipitation

Researcher: Fujita

Part: BBa_B0032 on pSB1C3

Electrophoresis

Researcher: Fujita, Noda

Part: BBa_B0032 on pSB1C3

Streaking (Single Colony Isolation)

Researcher: Shibata, Fujita

Part: BBa_R0011 on pSB1A2

Liquid Culture

Researcher: Shibata, Noda

Part: BBa_C0012 on pSB1C3, BBa_B0032 on pSB1C3, BBa_J23101 on BBa_J61002

2016/08/14

Mini-prep

Researcher: Nishimura, Kishimoto

Part: BBa_C0012 on pSB1C3, BBa_B0032 on pSB1C3, BBa_J23101 on BBa_J61002

PEG Precipitation

Researcher: Nishimura, Kono

Part: BBa_C0012 on pSB1C3, BBa_B0032 on pSB1C3, BBa_J23101 on BBa_J61002

Electrophoresis

Researcher: Nishimura, Kono

Part: BBa_C0012 on pSB1C3, BBa_B0032 on pSB1C3, BBa_J23101 on BBa_J61002

Transformation

Researcher: Nishimura, Kono, Donen

Part: BBa_R0011 on pSB1A2

2016/08/15

Gel Free System

Researcher: Fujita, Esashi

Part: BBa_C0012, BBa_J23101, BBa_B0032

Electrophoresis

Researcher: Kishida, Fujita, Donen, Shibata, Esashi

Part: BBa_C0012, BBa_J23101, BBa_B0032

Ethanol Precipitation

Researcher: Fujita, Kishida, Noda

Part: BBa_C0012, BBa_J23101, BBa_B0032

Liquid Culture

Researcher: Shibata

Part: BBa_R0011 on pSB1A2

2016/08/16

Electrophoresis

Researcher: Nishimura

Part: BBa_C0012, BBa_J23101, BBa_B0032

Mini-prep

Researcher: Nishimura

Part: BBa_R0011 on pSB1A2

PEG Precipitation

Researcher: Nishimura, Kono

Part: BBa_R0011 on pSB1A2

Electrophoresis

Researcher: Nishimura

Part: BBa_R0011 on pSB1A2

PCR (2 STEP)

Researcher: Esashi, Nishimura, Shibata

Part: BBa_R0011

Electrophoresis

Researcher: Nishimura

Part: BBa_R0011

2016/08/17

Gel Free System

Researcher: Sugishita, Shibata

Part: BBa_R0011

Electrophoresis

Researcher: Sugishita, Shibata, Kishida

Part: BBa_R0011

Gel Free System

Researcher: Sugishita, Kishida, Shibata

Part: BBa_R0011

PEG Precipitation

Researcher: Sugishita, Kishida, Shibata

Part: BBa_R0011

Transformation

Researcher: Nishimura

Part: BBa_I13504 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa_R0011

Transformation

Researcher: Shibata

Part: BBa_I13504 on pSB1C3

2016/08/18

Gel Free System

Researcher: Nishimura

Part: BBa_R0011

Ethanol Precipitation

Researcher:

Part: BBa_R0011

Electrophoresis

Researcher: Nishimura

Part: BBa_R0011

Liquid Culture

Researcher: Nishimura

Part: BBa_I13504 on pSB1C3

Transformation

Researcher: Kono

Part: BBa_I13504 on pSB1C3

2016/08/19

Mini-prep

Researcher: Shibata, Kishida

Part: BBa_I13504 on pSB1C3

PEG Precipitation

Researcher: Shibata

Part: BBa_I13504 on pSB1C3

Electrophoresis

Researcher: Shibata

Part: BBa_I13504 on pSB1C3

PCR (2 STEP)

Researcher: Nishimura

Part: BBa_I13504

Electrophoresis

Researcher: Shibata, Nishimura

Part: BBa_I13504

PCR (2 STEP)

Researcher: Nishimura, Shibata

Part: BBa_I13504

Electrophoresis

Researcher: Shibata, Nishimura

Part: BBa_I13504

2016/08/20

Gel Free System

Researcher: Fujita, Mimata

Part: BBa_I13504

Ethanol Precipitation

Researcher: Fujita, Mimata

Part: BBa_I13504

2016/08/21

Electrophoresis

Researcher: Nishimura, Mimata, Kishimoto

Part: BBa_I13504

Ligation

Researcher: Kishimoto, Nishimura, Mimata

Part: BBa_I13504 & BBa_R0011 on pSB1A2

Transformation

Researcher: Kishimoto, Kono, Mimata

Part: BBa_I13401 on pSB1A2, BBa_R0011 - BBa_I13504 on pSB1A2

2016/08/22

Colony PCR

Researcher: Mimata, Kono, Kishida, Esashi

Part: BBa_R0011 - BBa_I13504

Liquid Culture

Researcher: Mimata, Kono, Kishida, Esashi

Part: BBa_R0011 - BBa_I13504 on pSB1A2, BBa_I13401 on pSB1A2

2016/08/23

Mini-prep

Researcher: Mimata, Fujita

Part: BBa_I13401 on pSB1A2

PEG Precipitation

Researcher: Mimata, Fujita

Part: BBa_I13401 on pSB1A2, BBa_R0011 on pSB1A2

Digestion

Researcher: Mimata, Fujita, Yonezawa

Part: BBa_R0011 on pSB1A2

Gel Free System

Researcher: Mimata, Fujita, Yonezawa

Part: BBa_I13504

Ethanol Precipitation

Researcher: Mimata, Fujita, Yonezawa

Part: BBa_I13504

Electrophoresis

Researcher: Mimata, Fujita, Kishida, Kono

Part: BBa_R0011 on pSB1A2, BBa_I13504

Ligation

Researcher: Mimata, Fujita, Kishida, Kono

Part: BBa_R0011 on pSB1A2 & BBa_I13504

Electrophoresis

Researcher: Mimata, Fujita, Kishida, Kono

Part: BBa_R0011 - BBa_I13504 on pSB1A2

Transformation

Researcher: Mimata, Fujita, Kishida, Kono

Part: BBa_R0011 - BBa_I13504 on pSB1A2

Electrophoresis

Researcher: Mimata, Fujita, Kishida, Kono

Part: BBa_R0011 - BBa_I13504 on pSB1A2

2016/08/24

Colony PCR

Researcher: Fujita, Kono

Part: BBa_R0011 - BBa_I13504

Electrophoresis

Researcher: Fujita, Kono, Shibata, Mimata, Hirao

Part: BBa_R0011 - BBa_I13504

PCR (2 STEP)

Researcher: Kono, Shibata, Fujita

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015008, BBa_K2015009

Electrophoresis

Researcher: Fujita, Kono, Hirao

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015008, BBa_K2015009

Colony PCR

Researcher: Kono, Hirao

Part: BBa_R0011 - BBa_I13504

Digestion

Researcher: Mimata

Part: BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005

PCR (2 STEP)

Researcher: Fujita

Part: BBa_K2015000, BBa_K2015012, BBa_K2015008, BBa_K2015009

Electrophoresis

Researcher: Hirao

Part: BBa_R0011 - I13504

Electrophoresis

Researcher: Mimata

Part: BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Fujita

Part: BBa_K2015000, BBa_K2015012, BBa_K2015008, BBa_K2015009

Colony PCR

Researcher: Fujita, Mimata

Part: BBa_R0011 - BBa_I13504

Gel Extract

Researcher: Mimata

Part: BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Mimata

Part: BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005

Digestion

Researcher: Mimata, Fujita

Part: BBa_K2015000, pSB1C3

Electrophoresis

Researcher: Mimata, Fujita

Part: pSB1C3, BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Mimata, Fujita

Part: BBa_R0011 - I13504

2016/08/25

Digestion

Researcher: Mimata

Part: BBa_J04500, BBa_I13401

Gel Extract

Researcher: Mimata

Part: pSB1C3, BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Mimata

Part: BBa_J04500 on pSB1C3, BBa_I13401 on pSB1A2

Electrophoresis

Researcher: Noda, Mimata

Part: pSB1C3, BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005

Ethanol Precipitation

Researcher: Mimata, Fujita, Noda

Part: pSB1C3, BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Mimata, Fujita

Part: pSB1C3, BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Fujita

Part: pSB1C3, BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005

Ligation

Researcher: Fujita

Part: pSB1C3 & BBa_K2015000

Electrophoresis

Researcher: Fujita

Part: BBa_K2015000 on pSB1C3

PCR (2 STEP)

Researcher: Nishimura

Part: BBa_K2015012, BBa_K2015008

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015012, BBa_K2015008

Transformation

Researcher: Nishimura, Hirao

Part: BBa_K2015000 on pSB1C3

Gel Free System

Researcher: Shibata, Hirao

Part: BBa_I13401

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head)

PCR (2 STEP)

Researcher: Nishimura

Part: BBa_K2015008

Ethanol Precipitation

Researcher: Nishimura, Fujita

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015009

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015008

Gel Free System

Researcher: Fujita, Noda, Nishimura, Hirao

Part: BBa_I13401

Electrophoresis

Researcher: Hirao

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015009

2016/08/26

Colony PCR

Researcher: Nishimura

Part: BBa_K2015000

Ethanol Precipitation

Researcher: Hirao, Nishimura, Mimata

Part: BBa_I13401

Electrophoresis

Researcher: Hirao, Nishimura, Mimata

Part: BBa_K2015000

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head)

Digestion

Researcher: Hirao, Mimata, Kono

Part: BBa_K2015000

Electrophoresis

Researcher: Mimata, Kono

Part: BBa_K2015000

2016/08/27

Liquid Culture

Researcher: Mimata

Part: BBa_K2015000 on pSB1C3

PCR (2 STEP)

Researcher: Mimata, Hirao

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015008, BBa_K2015009

Digestion

Researcher: Hirao, Mimata, Noda

Part: BBa_K2015000

Electrophoresis

Researcher: Mimata, Noda, Hirao, Fujita, Shibata

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015008, BBa_K2015009

Ethanol Precipitation

Researcher: Fujita, Mimata, Shibata

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015008, BBa_K2015009

Digestion

Researcher: Fujita, Noda, Shibata, Hirao

Part: BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Hirao, Shibata

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015008, BBa_K2015009

Electrophoresis

Researcher: Hirao, Shibata, Noda

Part: BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005

Ethanol Precipitation

Researcher: Hirao, Shibata

Part: BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Shibata, Noda, Esashi

Part: BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005

Ligation

Researcher: Noda, Esashi, Shibata

Part: BBa_K2015005 - DNMT1 (head) & DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Noda, Esashi, Shibata

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

2016/08/28

Mini-prep

Researcher: Mimata

Part: BBa_K2015000 on pSB1C3

PEG Precipitation

Researcher: Fujita, Mimata, Hirao

Part: BBa_K2015000 on pSB1C3

Digestion

Researcher: Mimata, Hirao

Part: BBa_K2015009

PCR (2 STEP)

Researcher: Mimata, Hirao

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Ethanol Precipitation

Researcher: Mimata, Hirao

Part: BBa_K2015009

Electrophoresis

Researcher: Mimata, Nishimura, Hirao

Part: BBa_K2015009, BBa_K2015000 on pSB1C3

PCR (2 STEP)

Researcher: Mimata, Nishimura, Hirao

Part: BBa_K2015008

PCR (3 STEP)

Researcher: Mimata, Nishimura, Hirao

Part: BBa_K2015008

PCR (3 STEP)

Researcher: Mimata, Nishimura, Hirao

Part: BBa_K2015000

Electrophoresis

Researcher: Mimata, Hirao

Part: BBa_K2015005 - DNMT1 (head) & DNMT1 (tail) - BBa_K2015005, BBa_K2015008

2016/08/29

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015000 on pSB1C3

PCR (STEP DOWN)

Researcher: Nishimura

Part: BBa_K2015008

Colony PCR

Researcher: Nishimura

Part: BBa_K2015000/span>

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015008

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015000

PCR (2 STEP)

Researcher: Mimata, Kishida, Hirao

Part: BBa_K2015008

Electrophoresis

Researcher: Mimata, Kishida, Hirao

Part: BBa_K2015000

Electrophoresis

Researcher: Mimata, Kishida, Kono, Hirao

Part: BBa_K2015008

PCR (2 STEP)

Researcher: Kishida, Kono

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015008, BBa_K2015009

Electrophoresis

Researcher: Kono, Hirao

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015008, BBa_K2015009

Ethanol Precipitation

Researcher: Kishida, Hirao, Kono

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015008, BBa_K2015009

PCR (2 STEP)

Researcher: Kishida, Noda, Esashi

Part: BBa_K2015000, DNMT1 (tail) - BBa_K2015005, BBa_K2015012, BBa_K2015008

Ethanol Precipitation

Researcher: Noda, Esashi

Part: BBa_K2015008

Electrophoresis

Researcher: Noda, Esashi

Part: BBa_K2015000, DNMT1 (tail) - BBa_K2015005, BBa_K2015012, BBa_K2015008

Ethanol Precipitation

Researcher: Kono, Hirao

Part: BBa_K2015000, DNMT1 (tail) - BBa_K2015005, BBa_K2015012, BBa_K2015008

2016/08/30

PCR (2 STEP)

Researcher: Mimata

Part: BBa_K2015008

Digestion

Researcher: Hirao, Kono

Part: BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005

Ethanol Precipitation

Researcher: Kono, Hirao

Part: BBa_K2015005 - DNMT1 (head)

Electrophoresis

Researcher: Mimata

Part: BBa_K2015008

Electrophoresis

Researcher: Hirao, Kono

Part: BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005

Ligation

Researcher: Kono, Hirao

Part: BBa_K2015005 - DNMT1 (head) & DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Hirao, Kono, Mimata

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Ethanol Precipitation

Researcher: Mimata, Kono

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

PCR (2 STEP)

Researcher: Nishimura

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015009

Electrophoresis

Researcher: Shibata, Nishimura

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015009

Ethanol Precipitation

Researcher: Kishida, Shibata, Hirao

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015009

Electrophoresis

Researcher: Kishida, Hirao

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015009

Digestion

Researcher: Hirao, Kishida, Mimata

Part: BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005

Ethanol Precipitation

Researcher: Kishida, Mimata

Part: BBa_K2015005 - DNMT1 (head)

Electrophoresis

Researcher: Mimata, Nishimura

Part: BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005

Ligation

Researcher: Hirao, Kishida

Part: BBa_K2015005 - DNMT1 (head) & DNMT1 (tail) - BBa_K2015005

Ethanol Precipitation

Researcher: Hirao, Mimata

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

CompET15bent Cells

Researcher: Mimata, Hirao, Noda, Esashi, Kono, Fujita

2016/08/31

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

PCR (2 STEP)

Researcher: Nishimura, Hirao

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Digestion

Researcher: Kono, Nishimura, Mimata

Part: BBa_K2015000, BBa_K2015011, BBa_K2015012, BBa_K2015009, pSB1C3

Electrophoresis

Researcher: Nishimura, Noda

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Ethanol Precipitation

Researcher: Hirao, Mimata, Kishida

Part: BBa_K2015000, BBa_K2015011, BBa_K2015012, BBa_K2015009

Electrophoresis

Researcher: Nishimura, Noda

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Ethanol Precipitation

Researcher: Nishimura, Mimata, Noda, Kono

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Hirao, Kishida

Part: BBa_K2015000, BBa_K2015011, BBa_K2015012, BBa_K2015008, BBa_K2015009, pSB1C3

Electrophoresis

Researcher: Hirao

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Esashi

Part: BBa_K2015009 on pSB1C3, pSB1C3

Gel Extract

Researcher: Hirao, Kishida, Kono

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Hirao, Kono

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Transformation

Researcher: Kono

Part: pSB1C3

Digestion

Researcher: Esahsi, Mimata

Part: BBa_K2015000, BBa_K2015012, pSB1C3

PCR (2 STEP)

Researcher: Kono

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015009

PCR (2 STEP)

Researcher: Mimata

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Ethanol Precipitation

Researcher: Esashi

Part: BBa_K2015000, BBa_K2015012, pSB1C3

2016/09/01

Electrophoresis

Researcher: Mimata, Esashi, Kono

Part: BBa_K2015000, BBa_K2015012, BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005, pSB1C3

Ethanol Precipitation

Researcher: Kono, Mimata

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Ligation

Researcher: Mimata, Kono, Esashi

Part: BBa_K2015000 & pSB1C3, BBa_K2015012 & pSB1C3

Digestion

Researcher: Esashi, Mimata

Part: BBa_K2015009, pSB1C3

Electrophoresis

Researcher: Mimata, Esashi, Kono

Part: BBa_K2015009, BBa_K2015000 on pSB1C3, BBa_K2015012 on pSB1C3, BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005, pSB1C3

Ethanol Precipitation

Researcher: Kono, Mimata, Esashi

Part: BBa_K2015009, pSB1C3

Electrophoresis

Researcher: Hirao

Part: BBa_K2015009, BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005, pSB1C3

Transformation

Researcher: Nishimura

Part: BBa_K2015000 on pSB1C3, BBa_K2015012 on pSB1C3

Digestion

Researcher: Nishimura, Hirao, Shibata

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015012, BBa_K2015009

PCR (2 STEP)

Researcher: Noda, Nishimura

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015009, BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Noda

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015009

Electrophoresis

Researcher: Hirao, Shibata

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015009, BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Ethanol Precipitation

Researcher: Shibata, Hirao

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015012, BBa_K2015009

Ethanol Precipitation

Researcher: Shibata, Hirao

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015009

PCR (2 STEP)

Researcher: Nishimura, Kishida

Part: BBa_K2015002, BBa_K2015001

Colony PCR

Researcher: Kono

Part: pSB1C3

Electrophoresis

Researcher: Noda, Esashi

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015009, BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005, BBa_K2015002, BBa_K2015001

Ligation

Researcher: Nishimura, Esashi, Noda

Part: BBa_K2015009 & pSB1C3

Liquid Culture

Researcher: Kono

Part: pSB1C3

PCR (2 STEP)

Researcher: Nishimura, Noda

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015009

Electrophoresis

Researcher: Kono

Part: pSB1C3

Ethanol Precipitation

Researcher: Noda, Esashi, Nishimura

Part: BBa_K2015009 & pSB1C3

Gel Extract

Researcher: Nishimura, Hirao

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015009

Electrophoresis

Researcher: Hirao

Part: BBa_K2015009 on pSB1C3

PCR (3 STEP)

Researcher: Noda

Part: BBa_K2015002, BBa_K2015001

Transformation

Researcher: Nishimura

Part: BBa_K2015009 on pSB1C3

Gel Extract

Researcher: Hirao

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015009

2016/09/02

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015009

Electrophoresis

Researcher: Noda

Part: BBa_K2015002, BBa_K2015001

Digestion

Researcher: Nishimura

Part: BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005

PCR Purification

Researcher: Fujita

Part: BBa_K2015002, BBa_K2015001

Electrophoresis

Researcher: Fujita, Nishimura

Part: BBa_K2015002, BBa_K2015001, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005

Ethanol Precipitation

Researcher: Nishimura, Fujita

Part: BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Fujita, Noda

Part: BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005

Colony PCR

Researcher: Fujita

Part: BBa_K2015000 on pSB1C3, BBa_K2015012 on pSB1C3

Electrophoresis

Researcher: Fujita, Nishimura, Noda

Part: BBa_K2015000 on pSB1C3, BBa_K2015012 on pSB1C3

Ligation

Researcher: Fujita, Noda

Part: BBa_K2015005 - DNMT1 (head) & DNMT1 (tail) - BBa_K2015005

Ethanol Precipitation

Researcher: Nishimura, Noda

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Mini-prep

Researcher: Fujita, Nishimura

Part: pSB1C3

PEG Precipitation

Researcher: Fujita

Part: pSB1C3

Ethanol Precipitation

Researcher: Nishimura

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Fujita, Nishimura

Part: pSB1C3

Colony PCR

Researcher: Hirao, Fujita

Part: BBa_K2015000 on pSB1C3, BBa_K2015012 on pSB1C3

PCR (2 STEP)

Researcher: Fujita, Hirao

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Digestion

Researcher: Fujita, Hirao

Part: pSB1C3

Transformation

Researcher: Mimata, Hirao

Part: pSB1C3

Electrophoresis

Researcher: Hirao

Part: BBa_K2015012 on pSB1C3

Ethanol Precipitation

Researcher: Mimata

Part: pSB1C3

Electrophoresis

Researcher: Hiraa

Part: BBa_K2015012 on pSB1C3

Electrophoresis

Researcher: Mimata

Part: pSB1C3, BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Ethanol Precipitation

Researcher: Mimata, Hirao

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Digestion

Researcher: Nishimura

Part: pSB1C3

Electrophoresis

Researcher: Nishimura

Part: pSB1C3

Colony PCR

Researcher: Nishimura, Hirao

Part: BBa_K2015009 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015009 on pSB1C3

Electrophoresis

Researcher: Shibata, Hirap

Part: BBa_K2015009 on pSB1C3

2016/09/03

Electrophoresis

Researcher: Shibata, Hirap, Mimata

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Gel Extract

Researcher: Shibata

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Fujita

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Liquid Culture

Researcher: Fujita

Part: pSB1C3

PCR (2 STEP)

Researcher: Fujita, Hirao

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Transformation

Researcher: Mimata

Part: pSB1C3

Electrophoresis

Researcher: Nishimura, Hirao

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

2016/09/05

PCR (2 STEP)

Researcher: Hirao

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

PCR (2 STEP)

Researcher: Hirao, Shibata

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015009

Liquid Culture

Researcher: Mimata

Part: pSB1C3

Digestion

Researcher: Kishida

Part: pSB1C3, BBa_K2015011

Electrophoresis

Researcher: Kishida, Shibata

Part: pSB1C3, BBa_K2015011

Ethanol Precipitation

Researcher: Kishida, Shibata

Part: pSB1C3, BBa_K2015011

Electrophoresis

Researcher: Kishida, Shibata, Mimata

Part: pSB1C3, BBa_K2015011

Electrophoresis

Researcher: Hirao

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015009, BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Ligation

Researcher: Shibata, Kishida, Mimata

Part: pSB1C3 & BBa_K2015011

PCR (2 STEP)

Researcher: Mimata

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015009

Ethanol Precipitation

Researcher: Kishida, Shibata

Part: BBa_K2015011 on pSB1C3

Electrophoresis

Researcher: Kishida

Part: BBa_K2015011 on pSB1C3

Electrophoresis

Researcher: Kishida, Nishimura

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015009

PEG Precipitation

Researcher: Fujita, Mimata, Shibata, Kono

Part: pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015009

Dephosphorylation

Researcher: Shibata, Kono, Mimata

Part: pSB1C3

Digestion

Researcher: Shibata, Kono, Mimata

Part: BBa_K2015011

2016/09/06

Ethanol Precipitation

Researcher: Mimata

Part: BBa_K2015011

Electrophoresis

Researcher: Mimata, Kono

Part: BBa_K2015011, pSB1C3

Electrophoresis

Researcher: Kono

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015009

Mini-prep

Researcher: Kono, Shibata

Part: pSB1C3

Digestion

Researcher: Mimata, Shibata

Part: pSB1C3

PEG Precipitation

Researcher: Kono

Part: pSB1C3

Ethanol Precipitation

Researcher: Mimata

Part: pSB1C3

Dephosphorylation

Researcher: Mimata

Part: pSB1C3

Electrophoresis

Researcher: Mimata, Shibata, Kono

Part: pSB1C3

Electrophoresis

Researcher: Mimata, Shibata, Kono

Part: pSB1C3

Ligation

Researcher: Shibata, Mimata, Kono

Part: BBa_K2015011 & pSB1C3

Electrophoresis

Researcher: Mimata, Shibata

Part: BBa_K2015011 on pSB1C3

Transformation

Researcher: Mimata, Shibata

Part: BBa_K2015011 on pSB1C3

PCR (2 STEP)

Researcher: Kishida, Hirao

Part: BBa_K2015008

Electrophoresis

Researcher: Kishida, Hirao

Part: BBa_K2015008

Electrophoresis

Researcher: Hirao

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Gel Extract

Researcher: Kono, Hirao

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Hirao, Mimata

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Ethanol Precipitation

Researcher: Kishida, Mimata

Part: BBa_K2015008

PCR (2 STEP)

Researcher: Hirao

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Digestion

Researcher: Mimata, Hirao

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005, pET15b

Ethanol Precipitation

Researcher: Hirao

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005, pET15b

Electrophoresis

Researcher: Hirao, Mimata

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005, pET15b

Electrophoresis

Researcher: Shibata, Mimata, Kono

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005, pET15b

2016/09/07

Gel Extract

Researcher: Kono, Shibata, Mimata

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Shibata, Mimata, Kono

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Colony PCR

Researcher: Shibata, Mimata, Kono

Part: BBa_K2015011 on pSB1C3

Digestion

Researcher: Mimata, Kono, Shibata

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Shibata, Mimata, Kono

Part: BBa_K2015011

Ethanol Precipitation

Researcher: Mimata, Kono

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Mimata, Kono

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Dephosphorylation

Researcher: Shibata

Part: pET15b

Ligation

Researcher: Shibata

Part: pET15b & BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Colony PCR

Researcher: Shibata

Part: BBa_K2015011

Electrophoresis

Researcher: Shibata

Part: BBa_K2015011

Electrophoresis

Researcher: Shibata

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005 on pET15b, pET15b

Digestion

Researcher: Kishida

Part: pSB1C3, BBa_K2015000, BBa_K2015012

Ethanol Precipitation

Researcher: Kishida

Part: pSB1C3

Ethanol Precipitation

Researcher: Mimata

Part: pSB1C3, BBa_K2015000, BBa_K2015012

Electrophoresis

Researcher: Kishida

Part: pSB1C3

Digestion

Researcher: Mimata

Part: BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015008, BBa_K2015009, pSB1C3

Colony PCR

Researcher: Mimata

Part: BBa_K2015011

Digestion

Researcher: Kishida

Part: pSB1C3, BBa_K2015000, BBa_K2015012

Ethanol Precipitation

Researcher: Mimata

Part: pSB1C3, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015008, BBa_K2015009

Dephosphorylation

Researcher: Mimata

Part: pSB1C3

Ligation

Researcher: Mimata

Part: pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015008, BBa_K2015009, pSB1C3

Ethanol Precipitation

Researcher: Mimata

Part: pSB1C3, BBa_K2015000, BBa_K2015012

Electrophoresis

Researcher: Kishida

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015008, BBa_K2015009

Electrophoresis

Researcher: Kishida

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015008, BBa_K2015009

Electrophoresis

Researcher: Kishida, Mimata

Part: BBa_K2015011

Ligation

Researcher: Mimata

Part: BBa_K2015005 - DNMT1 (head) & DNMT1 (tail) - BBa_K2015005, BBa_K2015008 & pSB1C3, BBa_K2015009 & pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa_K2015000, BBa_K2015012, pSB1C3

Gel Extract

Researcher: Kishida, Hirao

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015008, BBa_K2015009

Transformation

Researcher: Mimata

Part: pSB1C3

Ligation

Researcher: Hirao

Part: BBa_K2015005 - DNMT1 (head) & DNMT1 (tail) - BBa_K2015005, BBa_K2015008 & pSB1C3, BBa_K2015009 & pSB1C3

Electrophoresis

Researcher: Mimata, Hirao

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005, BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3

2016/09/08

Gel Extract

Researcher: Mimata, Hirao

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005, BBa_K2015009 on pSB1C3

Transformation

Researcher: Hirao

Part: BBa_K2015009 on pSB1C3

Digestion

Researcher: Mimata, Hirao

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Ethanol Precipitation

Researcher: Shibata, Mimata

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015012, BBa_K2015008, BBa_K2015009, BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005, pSB1C3

Electrophoresis

Researcher: Shibata, Mimata

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015012, BBa_K2015008, BBa_K2015009, BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005, pSB1C3

Digestion

Researcher: Shibata

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015012, BBa_K2015008, BBa_K2015009, pSB1C3

Ligation

Researcher: Hirao, Mimata, Shibata

Part: BBa_K2015000 & pSB1C3, BBa_K2015005 - DNMT1 (head) & DNMT1 (tail) - BBa_K2015005, BBa_K2015012 & pSB1C3, BBa_K2015008 & pSB1C3, BBa_K2015009 & pSB1C3, BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005 & pET15b

Digestion

Researcher: Kishida

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015012, BBa_K2015008, BBa_K2015009, pSB1C3, pET15b

Ethanol Precipitation

Researcher: Kishida

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015012, BBa_K2015008, BBa_K2015009, pSB1C3

Electrophoresis

Researcher: Kishida, Mimata

Part: BBa_K2015000 on pSB1C3, BBa_K2015012 on pSB1C3, BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3, BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015012, BBa_K2015008, BBa_K2015009, BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005 on pET15b, pSB1C3

Colony PCR

Researcher: Hirao

Part: pSB1C3

Ethanol Precipitation

Researcher: Kishida

Part: pET15b

Ethanol Precipitation

Researcher: Mimata, Hirao

Part: BBa_K2015000 on pSB1C3, BBa_K2015012 on pSB1C3, BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3, BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005 on pET15b, BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Dephosphorylation

Researcher: Kishida, Hirao

Part: pSB1C3, pET15b

Transformation

Researcher: Hirao

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005 on pSB1C3, BBa_K2015012 on pSB1C3

Ligation

Researcher: Hirao

Part: BBa_K2015008 & pSB1C3

Electrophoresis

Researcher: Mimata

Part: pSB1C3

Transformation

Researcher: Mimata

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005 on pET15b

Digestion

Researcher: Shibata

Part: BBa_K2015000, BBa_K2015012, pSB1C3

Colony PCR

Researcher: Mimata

Part: BBa_K2015009

2016/09/09

Ethanol Precipitation

Researcher: Shibata

Part: BBa_K2015002, BBa_K2015001

Electrophoresis

Researcher: Mimata

Part: BBa_K2015008 on pSB1C3, BBa_K2015009

Transformation

Researcher: Mimata

Part: BBa_K2015008 on pSB1C3

Ethanol Precipitation

Researcher: Shibata

Part: BBa_K2015000, BBa_K2015012, pSB1C3

Digestion

Researcher: Mimata

Part: BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015008, BBa_K2015009, pSB1C3

Electrophoresis

Researcher: Shibata

Part: BBa_K2015000, BBa_K2015012, pSB1C3

Digestion

Researcher: Shibata

Part: BBa_K2015002, BBa_K2015001

Ethanol Precipitation

Researcher: Shibata

Part: BBa_K2015002, BBa_K2015001

Ethanol Precipitation

Researcher: Mimata

Part: BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015008, BBa_K2015009, pSB1C3

Electrophoresis

Researcher: Mimata

Part: pET15b

Electrophoresis

Researcher: Shibata

Part: BBa_K2015002, BBa_K2015001

Ligation

Researcher: Shibata

Part: BBa_K2015012, pSB1C3

Gel Extract

Researcher: Mimata

Part: pET15b

Electrophoresis

Researcher: Mimata

Part: BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015008, BBa_K2015009, pSB1C3, pET15b

Dephosphorylation

Researcher: Mimata

Part:pSB1C3, pET15b

Ligation

Researcher: Shibata

Part: BBa_K2015002 & pSB1C3, BBa_K2015001 & pSB1C3

Electrophoresis

Researcher: Mimata

Part: pSB1C3, pET15b

Ligation

Researcher: Mimata

Part: BBa_K2015008 & pSB1C3, BBa_K2015009 & pSB1C3

PCR (2 STEP)

Researcher: Kishida

Part: BBa_K2015002, BBa_K2015001

Electrophoresis

Researcher: Kishida

Part: BBa_K2015012 on pSB1C3, BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3, BBa_K2015002 on pSB1C3, BBa_K2015001 on pSB1C3

Colony PCR

Researcher: Mimata

Part: BBa_K2015012, BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005, BBa_K2015009

Liquid Culture

Researcher: Kono

Part: pSB1C3

Colony PCR

Researcher: Kono

Part: BBa_K2015012

PCR Purification

Researcher: Kishida

Part: BBa_K2015002, BBa_K2015001

Transformation

Researcher: Mimata

Part: BBa_K2015012 on pSB1C3, BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3

Ethanol Precipitation

Researcher: Mimata, Kono

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015009

Electrophoresis

Researcher: Mimata

Part: BBa_K2015012, BBa_K2015009, BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Kono

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015009

Electrophoresis

Researcher: Kono

Part: BBa_K2015012

Electrophoresis

Researcher: Mimata

Part: BBa_K2015008

Ethanol Precipitation

Researcher: Mimata

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, pET15b

2016/09/10

Digestion

Researcher: Kono

Part: BBa_K2015012, BBa_K2015009, pSB1C3

Ethanol Precipitation

Researcher: Kono

Part: BBa_K2015012, BBa_K2015009, pSB1C3

PCR (3 STEP)

Researcher: Shibata

Part: BBa_K2015002, BBa_K2015001

Electrophoresis

Researcher: Shibata

Part: BBa_K2015002, BBa_K2015001

Digestion

Researcher: Shibata

Part: pSB1C3

Ethanol Precipitation

Researcher: Shibata

Part: pSB1C3

PCR Purification

Researcher: Shibata

Part: BBa_K2015002, BBa_K2015001

Electrophoresis

Researcher: Shibata

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015012, BBa_K2015009, pSB1C3, pET15b, BBa_K2015002, BBa_K2015001

Mini-prep

Researcher: Shibata

Part: pSB1C3

PEG Precipitation

Researcher: Shibata

Part: pSB1C3

PCR (3 STEP)

Researcher: Mimata, Shibata

Part: BBa_K2015012, BBa_K2015008, BBa_K2015009

Ligation

Researcher: Mimata

Part: BBa_K2015005 - DNMT1 (head) & DNMT1 (tail) - BBa_K2015005

2016/09/11

Ethanol Precipitation

Researcher: Shibata

Part: BBa_K2015002, BBa_K2015001

Dephosphorylation

Researcher: Mimata

Part: pET15b

Electrophoresis

Researcher: Mimata

Part: BBa_K2015012, BBa_K2015008, BBa_K2015009

Electrophoresis

Researcher: Shibata

Part: pSB1C3

Electrophoresis

Researcher: Shibata

Part: BBa_K2015002, BBa_K2015001

Electrophoresis

Researcher: Mimata

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005, pET15b

Gel Extract

Researcher: Mimata

Part: pSB1C3

Ligation

Researcher: Mimata

Part: BBa_K2015000 & pSB1C3

Gel Extract

Researcher: Mimata

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Mimata

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005, pSB1C3

PCR (3 STEP)

Researcher: Shibata

Part: BBa_K2015002, BBa_K2015001

Electrophoresis

Researcher: Shibata

Part: BBa_K2015002, BBa_K2015001

PCR Purification

Researcher: Shibata

Part: BBa_K2015002, BBa_K2015001

Ethanol Precipitation

Researcher: Shibata

Part: BBa_K2015002, BBa_K2015001

Electrophoresis

Researcher: Shibata

Part: BBa_K2015002, BBa_K2015001

Ethanol Precipitation

Researcher: Shibata

Part: BBa_K2015002, BBa_K2015001

Electrophoresis

Researcher: Shibata

Part: BBa_K2015002, BBa_K2015001

Transformation

Researcher: Mimata

Part: BBa_K2015000 on pSB1C3

Digestion

Researcher: Mimata

Part: BBa_K2015011, pSB1C3

Transformation

Researcher: Mimata

Part: pET15b

Ethanol Precipitation

Researcher: Mimata

Part: BBa_K2015011, pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa_K2015011, pSB1C3

Dephosphorylation

Researcher: Mimata

Part: pSB1C3

2016/09/12

Ligation

Researcher: Mimata

Part: BBa_K2015011 & pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa_K2015011 on pSB1C3

Transformation

Researcher: Mimata

Part: BBa_K2015011 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: pET15b, pSB1C3

PCR (2 STEP)

Researcher: Nishimura

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015008, BBa_K2015009

Colony PCR

Researcher: Shibata

Part: BBa_K2015000

Digestion

Researcher: Nishimura

Part: pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015008, BBa_K2015009

Gel Extract

Researcher: Nishimura, Shibata

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015008, BBa_K2015009

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015008, BBa_K2015009

Gel Extract

Researcher: Nishimura, Shibata

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015008, BBa_K2015009

Electrophoresis

Researcher: Nishimura

Part: pSB1C3, BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015008, BBa_K2015009

2016/09/13

Digestion

Researcher: Nishimura, Mimata

Part: pSB1C3

Liquid Culture

Researcher: Mimata

Part: pSB1C3

Transformation

Researcher: Mimata

Part: pSB1C3

Electrophoresis

Researcher: Mimata

Part: pSB1C3

Electrophoresis

Researcher: Mimata, Nishimura

Part: pSB1C3

Digestion

Researcher: Nishimura, Mimata

Part: pSB1C3

Electrophoresis

Researcher: Mimata, Nishimura

Part: pSB1C3

Digestion

Researcher: Nishimura, Mimata

Part: BBa_K2015000, BBa_K2015008, BBa_K2015009

PCR (2 STEP)

Researcher: Nishimura, Hirao

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015008, BBa_K2015009

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015008, BBa_K2015009

Gel Extract

Researcher: Mimata, Hirao, Nishimura

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015008, BBa_K2015009

Digestion

Researcher: Nishimura

Part: BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015011, BBa_K2015012, BBa_K2015008, BBa_K2015009

Ethanol Precipitation

Researcher: Mimata, Nishimura

Part: BBa_K2015000, BBa_K2015008, BBa_K2015009

Electrophoresis

Researcher: Nishimura, Kishimoto

Part: BBa_K2015000, BBa_K2015008, BBa_K2015009

PCR (2 STEP)

Researcher: Nishimura

Part: BBa_K2015002, BBa_K2015001

Electrophoresis

Researcher: Nishimura, Kishimoto

Part: BBa_K2015002, BBa_K2015001

Ligation

Researcher: Nishimura

Part: BBa_K2015000 & pSB1C3, BBa_K2015009 & pSB1C3, BBa_K2015008 & BBa_K2015012 on pSB1C3

Electrophoresis

Researcher: Kono, Hirao

Part: BBa_K2015000 on pSB1C3, BBa_K2015009 on pSB1C3

2016/09/14

Transformation

Researcher: Shibata, Kono

Part: BBa_K2015000 on pSB1C3, BBa_K2015009 on pSB1C3

Digestion

Researcher: Kono, Shibata

Part: pSB1C3

Electrophoresis

Researcher: Nishimura, Kishimoto, Kishida, Hirao

Part: BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, pSB1C3

Ethanol Precipitation

Researcher: Nishimura, Kishida, Hirao, Kishimoto

Part: BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005

Digestion

Researcher: Nishimura, Hirao, Kishimoto

Part: pET15b

Ethanol Precipitation

Researcher: Nishimura, Kishida, Hirao, Kishimoto

Part: BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, pET15b

Transformation

Researcher: Nishimura

Part: BBa_K2015012 - BBa_K2015008 on pSB1C3

Digestion

Researcher: Nishimura

Part: DNMT1 (tail) - BBa_K2015005

PCR (2 STEP)

Researcher: Kishida, Nishimura, Kishimoto

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015008, BBa_K2015009

Ethanol Precipitation

Researcher: Nishimura, Kishida

Part: DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015008, BBa_K2015009

Gel Extract

Researcher: Nishimura, Mimata, Kishida

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015008, BBa_K2015009

Ligation

Researcher: Nishimura

Part: BBa_K2015005 - DNMT1 (head) & DNMT1 (tail) - BBa_K2015005

Colony PCR

Researcher: Mimata, Hirao, Shibata

Part: BBa_K2015009

Electrophoresis

Researcher: Shibata

Part: BBa_K2015000, BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005, BBa_K2015008

Electrophoresis

Researcher: Shibata, Hirao, Noda, Kono

Part: BBa_K2015009

2016/09/15

Liquid Culture

Researcher: Shibata, Noda, Kono

Part: pSB1C3

PCR (2 STEP)

Researcher: Kono, Noda

Part: BBa_K2015009

Electrophoresis

Researcher: Shibata, Noda, Kono

Part: BBa_K2015000

Electrophoresis

Researcher: Shibata, Kono

Part: BBa_K2015009

Gel Extract

Researcher: Shibata, Kono, Noda

Part: BBa_K2015009

Electrophoresis

Researcher: Shibata, Kono, Noda

Part: BBa_K2015009

Colony PCR

Researcher: Fujita

Part: BBa_K2015008

Electrophoresis

Researcher: Fujita

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Gel Extract

Researcher: Fujita

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Fujita

Part: BBa_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Fujita

Part: BBa_K2015008

Digestion

Researcher: Fujita

Part: BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005

Colony PCR

Researcher: Fujita

Part: BBa_K2015012 - BBa_K2015008

Ethanol Precipitation

Researcher: Nishimura

Part: BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005

Electrophoresis

Researcher: Kishimoto, Nishimura

Part: BBa_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa_K2015005

Colony PCR

Researcher: Nishimura

Part: BBa_K2015012 - BBa_K2015008

Colony PCR

Researcher: Fujita

Part: BBa_K2015012 - BBa_K2015008

Mini-prep

Researcher: Nishimura, Kishimoto

Part: BBa_K2015009 on pSB1C3

Mini-prep

Researcher: Fujita

Part: BBa_K2015000 on pSB1C3, pSB1C3

PEG Precipitation

Researcher: Fujita, Hirao

Part: BBa_K2015000 on pSB1C3, BBa_K2015009 on pSB1C3, pSB1C3

Electrophoresis

Researcher: Fujita, Hirao

Part: BBa_K2015012 - BBa_K2015008

Electrophoresis

Researcher: Fujita, Hirao

Part: BBa_K2015009 on pSB1C3, BBa_K2015000 on pSB1C3, pSB1C3

PCR (3 STEP)

Researcher: Fujita, Hirao

Part: BBa_K2015000, BBa_K2015009

Digestion

Researcher: Fujita, Hirao

Part: pSB1C3, BBa_K2015011

2016/09/16

Electrophoresis

Researcher: Fujita, Hirao

Part: pSB1C3, BBa_K2015011

Ethanol Precipitation

Researcher: Mimata, Fujita, Hirao

Part: BBa_K2015000, BBa_K2015009

Sequencing

Researcher: Fujita, Hirao, Mimata

Part: BBa_K2015000, BBa_K2015009

Ligation

Researcher: Hirao, Mimata, Fujita

Part: BBa_K2015011 & pSB1C3

Electrophoresis

Researcher: Mimata, Hirao, Kono

Part: BBa_K2015011 on pSB1C3

Transformation

Researcher: Mimata, Kono

Part: BBa_K2015011 on pSB1C3

Colony PCR

Researcher: Nishimura

Part: BBa_K2015012 - BBa_K2015008

Ligation

Researcher: Kono, Nishimura

Part: BBa_K2015005 - DNMT1 (head) & DNMT1 (tail) - BBa_K2015005

PEG Precipitation

Researcher: Kono

Part: BBa_K2015000 on pSB1C3

Electrophoresis

Researcher: Kono, Fujita, Nishimura

Part: BBa_K2015000 on pSB1C3

Electrophoresis

Researcher: Kono, Fujita, Nishimura

Part: BBa_K2015012 - BBa_K2015008 on pSB1A3

Sequencing

Researcher: Fujita, Kishida

Part: BBa_K2015000, BBa_K2015009

Liquid Culture

Researcher: Nishimura

Part: BBa_K2015012 on pSB1A3, BBa_K2015008 on pSB1A3

Ligation

Researcher: Nishimura

Part: BBa_K2015008 & pSB1C3

Ethanol Precipitation

Researcher: Fujita, Hirao, Shibata

Part: BBa_K2015000, BBa_K2015009

Colony PCR

Researcher: Kishida, Fujita, Shibata, Hirao

Part: BBa_K2015000, BBa_K2015011, BBa_K2015009

Transformation

Researcher: Fujita

Part: BBa_K2015008 on pSB1C3

2016/09/17

Electrophoresis

Researcher: Fujita, Hirao, Mimata

Part: BBa_K2015000, BBa_K2015011, BBa_K2015009

Liquid Culture

Researcher: Mimata

Part: BBa_K2015000 on pSB1C3, BBa_K2015009 on pSB1C3

Digestion

Researcher: Nishimura

Part: pSB1C3

Mini-prep

Researcher: Nishimura

Part: BBa_K2015000 on pSB1C3, BBa_K2015012 - BBa_K2015008 on pSB1A3

PEG Precipitation

Researcher: Nishimura

Part: BBa_K2015000 on pSB1C3, BBa_K2015012 - BBa_K2015008 on pSB1A3

Electrophoresis

Researcher: Nishimura, Kishida

Part: BBa_K2015000 on pSB1C3, BBa_K2015012 - BBa_K2015008 on pSB1A3, BBa_K2015011

Ligation

Researcher: Nishimura

Part: BBa_K2015011 & pSB1C3

Electrophoresis

Researcher: Kishida

Part: BBa_K2015000 on pSB1C3, BBa_K2015012 - BBa_K2015008 on pSB1A3

Transformation

Researcher: Kishida

Part: BBa_K2015011 on pSB1C3

Sequencing

Researcher: Shidata, Nishimura

Part: BBa_K2015000, BBa_K2015009

Colony PCR

Researcher: Nishimura

Part: BBa_K2015011

Transformation

Researcher: Nishimura

Part: BBa_K2015011 on pSB1C3

Colony PCR

Researcher: Nishimura, Shibata

Part: BBa_K2015008

Digestion

Researcher: Shibata

Part: BBa_K2015011, pSB1C3

Ethanol Precipitation

Researcher: Shibata, Nishimura

Part: BBa_K2015011, pSB1C3

Electrophoresis

Researcher: Kono, Nishimura, Shibata

Part: BBa_K2015011, pSB1C3

Ethanol Precipitation

Researcher: Mimata, Kono

Part: BBa_K2015000, BBa_K2015012 - BBa_K2015008, BBa_K2015009

Digestion

Researcher: Mimata, Kono

Part: BBa_K2015011

2016/09/18

Ethanol Precipitation

Researcher: Shibata, Mimata, Kono

Part: BBa_K2015011

Mini-prep

Researcher: Mimata, Shibata, Kono

Part: BBa_K2015000 on pSB1C3, BBa_K2015009 on pSB1C3

Electrophoresis

Researcher: Kono, Mimata, Shibata

Part: BBa_K2015000 on pSB1C3, BBa_K2015009 on pSB1C3, BBa_K2015011

PEG Precipitation

Researcher: Mimata, Shibata, Kono

Part: BBa_K2015000 on pSB1C3, BBa_K2015009 on pSB1C3

Electrophoresis

Researcher: Kono, Mimata, Fujita

Part: BBa_K2015008

Ligation

Researcher: Fujita, Kono, Mimata

Part: BBa_K2015011 & pSB1C3

Liquid Culture

Researcher: Mimata, Kono

Part: BBa_K2015008 on pSB1C3

Electrophoresis

Researcher: Kono, Mimata, Fujita

Part: BBa_K2015000 on pSB1C3, BBa_K2015009 on pSB1C3

Sequencing

Researcher: Mimata, Fujita, Kono

Part: BBa_K2015000, BBa_K2015009

Electrophoresis

Researcher: Mimata, Fujita

Part: BBa_K2015011 on pSB1C3

Liquid Culture

Researcher: Kono

Part: BBa_K2015000 on pSB1C3

Transformation

Researcher: Mimata

Part: BBa_K2015011 on pSB1C3

Ethanol Precipitation

Researcher: Fujita

Part: BBa_K2015000, BBa_K2015009

Colony PCR

Researcher: Nishimura, Hirao, Kishimoto

Part: BBa_K2015011

Digestion

Researcher: Nishimura, Hirao, Kishimoto

Part: BBa_K2015009

Colony PCR

Researcher: Nishimura, Kishimoto

Part: BBa_K2015009

Ethanol Precipitation

Researcher: Nishimura, Kishimoto

Part: BBa_K2015009

Electrophoresis

Researcher: Mimata

Part: BBa_K2015009

Electrophoresis

Researcher: Esashi

Part: BBa_K2015011

Mini-prep

Researcher: Mimata, Hirao

Part: BBa_K2015008

Electrophoresis

Researcher: Noda

Part: BBa_K2015008

Ligation

Researcher: Mimata, Hirao

Part: BBa_K2015009 & pSB1C3

Mini-prep

Researcher: Mimata, Hirao

Part: BBa_K2015000 on pSB1C3

Electrophoresis

Researcher: Noda

Part: BBa_K2015000 on pSB1C3

PEG Precipitation

Researcher: Mimata, Hirao

Part: BBa_K2015000 on pSB1C3

Electrophoresis

Researcher: Esashi

Part: BBa_K2015009 on pSB1C3

Sequencing

Researcher: Mimata, Hirao

Part: BBa_K2015009

Electrophoresis

Researcher: Hirao, Mimata

Part: BBa_K2015000 on pSB1C3, BBa_K2015009 on pSB1C3

Transformation

Researcher: Mimata, Hirao

Part: BBa_K2015009 on pSB1C3

2016/09/19

Sequencing

Researcher: Mimata, Hirao

Part: BBa_K2015000

Colony PCR

Researcher: Mimata, Shibata, Hirao

Part: BBa_K2015011

Electrophoresis

Researcher: Shibata

Part: BBa_K2015011

Liquid Culture

Researcher: Mimata

Part: BBa_K2015009 on pSB1C3

Ethanol Precipitation

Researcher: Mimata, Shibata, Hirao

Part: BBa_K2015000, BBa_K2015009

Liquid Culture

Researcher: Nishimura

Part: BBa_K2015011 on pSB1C3

PEG Precipitation

Researcher: Shibata

Part: BBa_K2015008 on pSB1C3

Electrophoresis

Researcher: Shibata

Part: BBa_K2015008 on pSB1C3

Sequencing

Researcher: Shibata

Part: BBa_K2015008

Ethanol Precipitation

Researcher: Nishimura

Part: BBa_K2015008

Sequencing

Researcher: Nishimura

Part: BBa_K2015000, BBa_K2015009

PEG Precipitation

Researcher: Nishimura, Kishida

Part: BBa_K2015000 on pSB1C3

Mini-prep

Researcher: Nishimura, Kishida

Part: BBa_K2015011 on pSB1C3

Liquid Culture

Researcher: Nishimura

Part: BBa_K2015000 on pSB1C3

PEG Precipitation

Researcher: Nishimura

Part: BBa_K2015000 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015011 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015000 on pSB1C3

Ethanol Precipitation

Researcher: Nishimura

Part: BBa_K2015000, BBa_K2015009

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015000 on pSB1C3, BBa_K2015011 on pSB1C3

Mini-prep

Researcher: Nishimura, Mimata

Part: BBa_K2015009 on pSB1C3

Electrophoresis

Researcher: Nishimura, Mimata

Part: BBa_K2015009 on pSB1C3

PEG Precipitation

Researcher: Mimata, Nishimura

Part: BBa_K2015009 on pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa_K2015009 on pSB1C3

Colony PCR

Researcher: Mimata, Fujita

Part: BBa_K2015008, BBa_K2015009

Sequencing

Researcher: Mimata, Fujita

Part: BBa_K2015000, BBa_K2015011, BBa_K2015009

2016/09/20

Electrophoresis

Researcher: Mimata, Fujita

Part: BBa_K2015008, BBa_K2015009

Ethanol Precipitation

Researcher: Mimata, Fujita

Part: BBa_K2015000, BBa_K2015011, BBa_K2015009

Mini-prep

Researcher: Mimata, Fujita

Part: BBa_K2015011 on pSB1C3

Colony PCR

Researcher: Mimata, Fujita

Part: BBa_K2015008, BBa_K2015009

Mini-prep

Researcher: Mimata, Fujita

Part: BBa_K2015000 on pSB1C3

Electrophoresis

Researcher: Mimata, Fujita

Part: BBa_K2015000 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015012, BBa_K2015008

Digestion

Researcher: Nishimura

Part: BBa_K2015012

Ethanol Precipitation

Researcher: Nishimura

Part: BBa_K2015012

Colony PCR

Researcher: Nishimura

Part: BBa_K2015008, BBa_K2015009

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015012

Ligation

Researcher: Nishimura

Part: BBa_K2015012 & pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015008, BBa_K2015009

Transformation

Researcher: Nishimura

Part: BBa_K2015012 on pSB1C3

PEG Precipitation

Researcher: Nishimura

Part: BBa_K2015000 on pSB1C3, BBa_K2015011 on pSB1C3, BBa_K2015012 on pSB1A3

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015011 on pSB1C3

Sequencing

Researcher: Fujita, Kono

Part: BBa_K2015000, BBa_K2015011, BBa_K2015009, BBa_K2015012 - BBa_K2015008

2016/09/21

PEG Precipitation

Researcher: Fujita, Kono

Part: BBa_K2015000 on pSB1C3, BBa_K2015012 on pSB1A3, BBa_K2015012 - BBa_K2015008 on pSB1A3

Colony PCR

Researcher: Fujita, Kono

Part: BBa_K2015008

Electrophoresis

Researcher: Fujita, Kono, Hirao

Part: BBa_K2015000 on pSB1C3, BBa_K2015012 on pSB1A3, BBa_K2015012 - BBa_K2015008 on pSB1A3

Ethanol Precipitation

Researcher: Shibata, Mimata, Hirao

Part: BBa_K2015000, BBa_K2015011, BBa_K2015009, BBa_K2015012 - BBa_K2015008

Sequencing

Researcher: Mimata, Shibata, Hirao

Part: BBa_K2015000, BBa_K2015011, BBa_K2015012 - BBa_K2015008

Electrophoresis

Researcher: Hirao

Part: BBa_K2015008

Ethanol Precipitation

Researcher: Shibata, Mimata, Hirao

Part: BBa_K2015000, BBa_K2015011, BBa_K2015012 - BBa_K2015008

Liquid Culture

Researcher: Nishimura

Part: BBa_K2015008 on pSB1C3

Colony PCR

Researcher: Nishimura, Kono

Part: BBa_K2015009

PEG Precipitation

Researcher: Nishimura

Part: BBa_K2015000

Electrophoresis

Researcher: Kono

Part: BBa_K2015009

PEG Precipitation

Researcher: Mimata, Kono

Part: BBa_K2015009

Electrophoresis

Researcher: Mimata, Kono

Part: BBa_K2015000, BBa_K2015009

Sequencing

Researcher: Mimata, Kono, Esashi

Part: BBa_K2015000, BBa_K2015011, BBa_K2015012 - BBa_K2015008, BBa_K2015009

2016/09/22

Ethanol Precipitation

Researcher: Mimata, Shibata, Noda

Part: BBa_K2015000, BBa_K2015011, BBa_K2015012 - BBa_K2015008, BBa_K2015009

Digestion

Researcher: Mimata

Part: BBa_K2015012

Digestion

Researcher: Mimata

Part: BBa_I13401

Ethanol Precipitation

Researcher: Mimata, Shibata

Part: BBa_I13401, BBa_K2015012 on pSB1A3

Electrophoresis

Researcher: Noda

Part: BBa_I13401, BBa_K2015012 on pSB1A3

Mini-prep

Researcher: Nishimura

Part: BBa_K2015008 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015008 on pSB1A3

Transformation

Researcher: Nishimura

Part: BBa_I13401 on pSB1C3

PEG Precipitation

Researcher: Nishimura

Part: BBa_K2015008 on pSB1C3

Sequencing

Researcher: Nishimura

Part: BBa_K2015000, BBa_K2015011, BBa_K2015012 - BBa_K2015008, BBa_K2015008, BBa_K2015009

Electrophoresis

Researcher: Hirao

Part: BBa_K2015008 on pSB1A3

Colony PCR

Researcher: Nishimura

Part: BBa_K2015009

Ethanol Precipitation

Researcher: Nishimura

Part: BBa_K2015000, BBa_K2015011, BBa_K2015012 - BBa_K2015008, BBa_K2015008, BBa_K2015009

Electrophoresis

Researcher: Hirao, Nishimura

Part: BBa_K2015009 on pSB1C3

Liquid Culture

Researcher: Nishimura, Hirao

Part: BBa_K2015000 on pSB1C3

PEG Precipitation

Researcher: Nishimura

Part: BBa_K2015008 on pSB1C3

2016/09/23

Colony PCR

Researcher: Fujita, Shibata

Part: BBa_K2015008

Digestion

Researcher: Nishimura, Shibata

Part: BBa_K2015011, pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015008 on pSB1C3

Electrophoresis

Researcher: Shibata

Part: BBa_K2015011, pSB1C3

Electrophoresis

Researcher: Fujita

Part: BBa_K2015008

Ethanol Precipitation

Researcher: Shibata

Part: BBa_K2015011

Transformation

Researcher: Nishimura

Part: BBa_B0015 on pSB1C3

Ligation

Researcher: Shibata, Fujita

Part: BBa_K2015011 & pSB1C3

Transformation

Researcher: Shibata

Part: BBa_K2015011 on pSB1C3

Mini-prep

Researcher: Nishimura

Part: BBa_K2015012 on pSB1A3

Liquid Culture

Researcher: Nishimura

Part: BBa_I13401 on pSB1A2

PEG Precipitation

Researcher: Nishimura

Part: BBa_K2015012 on pSB1A3

Colony PCR

Researcher: Nishimura

Part: BBa_K2015011

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015012 on pSB1A3

Mini-prep

Researcher: Nishimura, Kishimoto

Part: BBa_K2015000 on pSB1C3

PEG Precipitation

Researcher: Nishimura, Kishida, Kishimoto

Part: BBa_K2015000 on pSB1C3

Transformation

Researcher: Nishimura, Kishimoto

Part: BBa_B0015 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015000 on pSB1C3

Electrophoresis

Researcher: Hirao

Part: BBa_K2015011

Liquid Culture

Researcher: Nishimura

Part: BBa_K2015012 - BBa_K2015008 on pSB1A3, BBa_K2015009 on pSB1C3

Digestion

Researcher: Nishimura

Part: BBa_K2015012, pSB1C3

2016/09/24

Colony PCR

Researcher: Mimata

Part: BBa_K2015011

Sequencing

Researcher: Nishimura

Part: BBa_K2015000, BBa_K2015011, BBa_K2015008

Ethanol Precipitation

Researcher: Fujita

Part: BBa_K2015012, pSB1C3

Electrophoresis

Researcher: Fujita

Part: BBa_K2015012, pSB1C3

Mini-prep

Researcher: Mimata

Part: BBa_K2015012 - BBa_K2015008 on pSB1A3, BBa_K2015009 on pSB1C3

PEG Precipitation

Researcher: Mimata

Part: BBa_K2015012 - BBa_K2015008 on pSB1A3, BBa_K2015009 on pSB1C3

Electrophoresis

Researcher: Fujita

Part: BBa_K2015012 - BBa_K2015008 on pSB1A3, BBa_K2015009 on pSB1C3, pSB1C3

Digestion

Researcher: Nishimura

Part: pSB1C3

Digestion

Researcher: Mimata, Fujita

Part: BBa_K2015012 on pSB1A3, BBa_K2015009 on pSB1C3

Electrophoresis

Researcher: Fujita, Mimata

Part: BBa_K2015011

Liquid Culture

Researcher: Fujita

Part: BBa_K2015000 on pSB1C3, BBa_I13401 on pSB1A2

Electrophoresis

Researcher: Fujita

Part: BBa_K2015012 - BBa_K2015008 on pSB1A3, BBa_K2015009 on pSB1C3

Electrophoresis

Researcher: Hirao, Shibata

Part: BBa_K2015012 on pSB1A3, BBa_K2015009 on pSB1C3

Ligation

Researcher: Hirao, Shibata

Part: BBa_K2015012 & BBa_K2015009 on pSB1C3, BBa_K2015012 & pSB1C3

Electrophoresis

Researcher: Hirao, Shibata

Part: BBa_K2015012 - BBa_K2015009 on pSB1C3, BBa_K2015012 on pSB1C3

Transformation

Researcher: Kishida

Part: BBa_K2015012 on pSB1C3, BBa_B0015 on pSB1C3

Sequencing

Researcher: Nishimura

Part: BBa_K2015000, BBa_K2015011, BBa_K2015008

Digestion

Researcher: Shibata, Nishimura

Part: BBa_K2015009 on pSB1C3

Electrophoresis

Researcher: Shibata

Part: BBa_K2015009 on pSB1C3

Liquid Culture

Researcher: Shibata

Part: BBa_K2015011 on pSB1C3

2016/09/25

Ligation

Researcher: Shibata

Part: BBa_K2015012 & BBa_K2015009 on pSB1C3

Transformation

Researcher: Mimata

Part: BBa_K2015012 - BBa_K2015009 on pSB1C3

Colony PCR

Researcher: Mimata

Part: BBa_K2015011

Electrophoresis

Researcher: Mimata

Part: BBa_K2015011

Mini-prep

Researcher: Mimata, Fujita

Part: BBa_K2015000 on pSB1C3, BBa_I13401 on pSB1A2

Electrophoresis

Researcher: Mimata

Part: BBa_K2015000 on pSB1C3, BBa_I13401 on pSB1A2

PEG Precipitation

Researcher: Nishimura, Fujita

Part: BBa_K2015000 on pSB1C3, BBa_I13401 on pSB1A2

PEG Precipitation

Researcher: Mimata, Fujita

Part: BBa_K2015011 on pSB1C3

Transformation

Researcher: Mimata, Hirao

Part: BBa_B0015 on pSB1C3, BBa_I13401 on pSB1C3

PCR (2 STEP)

Researcher: Nishimura

Part: BBa_I13401, BBa_K2015012 - BBa_K2015008

Electrophoresis

Researcher: Hirao

Part: BBa_K2015011

Colony PCR

Researcher: Mimata

Part: BBa_K2015012

Electrophoresis

Researcher: Hirao

Part: BBa_I13401, BBa_K2015012 - BBa_K2015008

Liquid Culture

Researcher: Nishimura

Part: BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015012

PCR (3 STEP)

Researcher: Nishimura

Part: BBa_K2015009, BBa_K2015012 - BBa_K2015008

Liquid Culture

Researcher: Hirao

Part: BBa_K2015012 on pSB1C3, BBa_K2015012 - BBa_K2015008 on pSB1C3

2016/09/26

Ligation

Researcher: Nishimura, Hirao

Part: BBa_K2015012 & BBa_K2015009 on pSB1C3

Electrophoresis

Researcher: Hirao

Part: BBa_K2015012 - BBa_K2015008, BBa_K2015009

Transformation

Researcher: Mimata

Part: BBa_K2015012 - BBa_K2015009 on pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa_K2015012 - BBa_K2015008, BBa_K2015009

Gel Extract

Researcher: Mimata

Part: BBa_K2015012 - BBa_K2015008, BBa_K2015009

Electrophoresis

Researcher: Mimata

Part: BBa_K2015012 - BBa_K2015008, BBa_K2015009

Transformation

Researcher: Mimata

Part: BBa_B0015 on pSB1C3, BBa_I13401 on pSB1C3

Mini-prep

Researcher: Mimata

Part: BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3

PEG Precipitation

Researcher: Mimata

Part: BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3

Colony PCR

Researcher: Mimata, Shibata

Part: BBa_K2015011

Digestion

Researcher: Nishimura

Part: BBa_K2015012 on pSB1C3, BBa_I13401

PCR (3 STEP)

Researcher: Nishimura

Part: BBa_I13401

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015012 on pSB1A3, BBa_I13401

Mini-prep

Researcher: Hirao, Shibata, Kishida

Part: BBa_K2015012 on pSB1C3, BBa_K2015012 - BBa_K2015008 on pSB1A3

Electrophoresis

Researcher: Noda

Part: BBa_K2015011 on pSB1C3, BBa_K2015012 on pSB1C3, BBa_K2015012 - BBa_K2015008 on pSB1A3, BBa_I13401

Ethanol Precipitation

Researcher: Nishimura

Part: BBa_I13401

PEG Precipitation

Researcher: Nishimura

Part: BBa_K2015012 on pSB1C3, BBa_K2015012 - BBa_K2015008 on pSB1A3

PEG Precipitation

Researcher: Nishimura

Part: BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3, BBa_K2015012 - BBa_K2015008 on pSB1A3

Digestion

Researcher: Nishimura

Part: pSB1C3

Liquid Culture

Researcher: Kishimoto

Part: BBa_K2015011 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015012 on pSB1C3, BBa_K2015012 - BBa_K2015008 on pSB1A3, pSB1C3

Electrophoresis

Researcher: Nishimura, Kishimoto, Kono

Part: BBa_K2015000 on pSB1C3, BBa_K2015012 on pSB1C3, BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3, BBa_K2015012 - BBa_K2015008 on pSB1A3

Liquid Culture

Researcher: Nishimura, Kishimoto, Hirao

Part: BBa_K2015012 on pSB1C3, BBa_K2015012 - BBa_K2015008 on pSB1A3

2016/09/27

Digestion

Researcher: Mimata

Part: pSB1C3

Electrophoresis

Researcher: Mimata

Part: pSB1C3

Colony PCR

Researcher: Mimata

Part: BBa_K2015012, BBa_K2015009

Digestion

Researcher: Mimata

Part: pSB1C3

Transformation

Researcher: Nishimura, Mimata

Part: BBa_B0015

Electrophoresis

Researcher: Mimata

Part: pSB1C3

Liquid Culture

Researcher: Nishimura

Part: BBa_K2015012 - BBa_K2015008 on pSB1A3

Electrophoresis

Researcher: Mimata

Part: BBa_K2015012, BBa_K2015009

Digestion

Researcher: Mimata

Part: BBa_I13401, BBa_K2015012 on pSB1A3

Mini-prep

Researcher: Mimata

Part: BBa_K2015011 on pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa_K2015011 on pSB1C3, BBa_I13401, BBa_K2015012 on pSB1A3

PEG Precipitation

Researcher: Mimata, Nishimura

Part: BBa_K2015011 on pSB1C3

Liquid Culture

Researcher: Mimata

Part: BBa_K2015012 - BBa_K2015009 on pSB1C3

Mini-prep

Researcher: Mimata

Part: BBa_K2015012 - BBa_K2015008 on pSB1A3, BBa_K2015012 on pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa_K2015012 - BBa_K2015008 on pSB1A3, BBa_K2015012 on pSB1C3, BBa_K2015011 on pSB1C3

PEG Precipitation

Researcher: Mimata, Nishimura

Part: BBa_K2015012 - BBa_K2015008 on pSB1A3, BBa_K2015012 on pSB1C3

Gel Extract

Researcher: Nishimura

Part: BBa_I13401

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015012 - BBa_K2015008 on pSB1A3, BBa_K2015012 on pSB1C3, BBa_I13401

Sequencing

Researcher: Nishimura

Part: BBa_K2015011, BBa_K2015012

Ligation

Researcher: Shibata

Part: BBa_K2015012 on pSB1A3 & BBa_I13401

PEG Precipitation

Researcher: Fujita, Hirao, Kishimoto

Part: BBa_K2015012 on pSB1C3, BBa_K2015012 - BBa_K2015008 on pSB1A3, BBa_K2015011 on pSB1C3

Transformation

Researcher: Kishimoto, Hirao

Part: BBa_K2015012 - BBa_I13401 on pSB1A3

Liquid Culture

Researcher: Kishimoto, Hirao

Part: BBa_K2015012 on pSB1C3, BBa_K2015011 on pSB1C3

Electrophoresis

Researcher: Kishimoto, Hirao

Part: BBa_K2015012 - BBa_K2015008 on pSB1A3, BBa_K2015012 on pSB1C3, BBa_K2015011 on pSB1C3

2016/09/28

Mini-prep

Researcher: Mimata

Part: BBa_B0015 on pSB1C3, BBa_K2015012 - BBa_K2015009 on pSB1C3

PEG Precipitation

Researcher: Mimata, Nishimura

Part: BBa_K2015012 - -BBa_K2015009 on pSB1C3

Ethanol Precipitation

Researcher: Nishimura

Part: BBa_K2015011, BBa_K2015012

Liquid Culture

Researcher: Nishimura, Kishimoto, Kono

Part: BBa_K2015012 - BBa_K2015009 on pSB1C3

Electrophoresis

Researcher: Nishimura, Kishimoto, Kono

Part: BBa_K2015012 - BBa_K2015009 on pSB1C3

Streaking (Single Colony Isolation)

Researcher: Mimata

Part: BBa_K2015012 - BBa_I13401 on pSB1A3

Digestion

Researcher: Mimata, Fujita

Part: BBa_K2015012 on pSB1A3, BBa_K2015009

Mini-prep

Researcher: Kishida

Part: BBa_K2015012 on pSB1C3, BBa_K2015011 on pSB1C3

Electrophoresis

Researcher: Kishida

Part: BBa_K2015012 on pSB1C3, BBa_K2015011 on pSB1C3

Sequencing

Researcher: Nishimura

Part: BBa_K2015011, BBa_K2015012

Electrophoresis

Researcher: Mimata

Part: BBa_K2015012 on pSB1A3, BBa_K2015009

Electrophoresis

Researcher: Kishida

Part: BBa_K2015012 on pSB1C3, BBa_K2015011 on pSB1C3

Ligation

Researcher: Fujita

Part: BBa_K2015012 on pSB1A3 & BBa_K2015009

Transformation

Researcher: Fujita

Part: BBa_K2015012 - BBa_K2015009 on pSB1A3

2016/09/29

Ethanol Precipitation

Researcher: Nishimura

Part: BBa_K2015011, BBa_K2015012

Mini-prep

Researcher: Kono

Part: BBa_B0015 on pSB1C3, BBa_K2015012 - BBa_K2015008 on pSB1A3

Electrophoresis

Researcher: Mimata, Kono

Part: BBa_B0015 on pSB1C3, BBa_K2015012 - BBa_K2015008 on pSB1A3

PEG Precipitation

Researcher: Mimata, Kono

Part: BBa_B0015 on pSB1C3, BBa_K2015012 - BBa_K2015008 on pSB1A3

Streaking (Single Colony Isolation)

Researcher: Kono, Mimata

Part: BBa_K2015012 - BBa_K2015009 on pSB1A3

Electrophoresis

Researcher: Shibata

Part: BBa_B0015 on pSB1C3, BBa_K2015012 - BBa_K2015008 on pSB1A3

PCR (2 STEP)

Researcher: Nishimura

Part: BBa_K2015013

Streaking (Single Colony Isolation)

Researcher: Hirao

Part: BBa_K2015012 - BBa_I13401 on pSB1A3

Electrophoresis

Researcher: Hirao, Kishida

Part: BBa_K2015013

PCR (3 STEP)

Researcher: Nishimura, Hirao

Part: BBa_K2015013

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015013

PCR (2 STEP)

Researcher: Nishimura

Part: BBa_B0015

Electrophoresis

Researcher: Nishimura

Part: BBa_B0015

Mini-prep

Researcher: Shibata

Part: BBa_K2015012 - BBa_I13401 on pSB1A3

PEG Precipitation

Researcher: Shibata

Part: BBa_K2015012 - BBa_I13401 on pSB1A3

2016/09/30

Electrophoresis

Researcher: Shibata

Part: BBa_K2015012 - BBa_I13401 on pSB1A3

Digestion

Researcher: Nishimura

Part: BBa_K2015012 - BBa_K2015008 on pSB1A3, BBa_B0015 on pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa_K2015012 - BBa_K2015008 on pSB1A3, BBa_B0015 on pSB1C3

Gel Extract

Researcher: Mimata

Part: BBa_K2015012 - BBa_K2015008 on pSB1A3

PCR (STEP DOWN)

Researcher: Mimata

Part: BBa_K2015013

Electrophoresis

Researcher: Mimata, Shibata

Part: BBa_K2015012 - BBa_K2015008 on pSB1A3

Electrophoresis

Researcher: Mimata, Shibata

Part: BBa_K2015013

Liquid Culture

Researcher: Mimata

Part: BBa_K2015012 - BBa_I13401 on pSB1A3

PEG Precipitation

Researcher: Nishimura

Part: BBa_K2015012 - BBa_K2015009 on pSB1C3, BBa_B0015 on pSB1C3

Gel Extract

Researcher: Shibata

Part: BBa_K2015012 - BBa_K2015008

Electrophoresis

Researcher: Mimata, Shibata

Part: BBa_K2015012 - BBa_K2015008 on pSB1A3, BBa_K2015012 - BBa_K2015009 on pSB1C3, BBa_B0015 on pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa_K2015012 - BBa_K2015009 on pSB1C3, BBa_B0015 on pSB1C3

2016/10/01

PCR (2 STEP)

Researcher: Mimata

Part: BBa_K2015011

Digestion

Researcher: Mimata

Part: BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3, pSB1C3

Gel Free System

Researcher: Mimata, Kishimoto

Part: BBa_J04500

PEG Precipitation

Researcher: Mimata, Kishimoto, Hirao

Part: BBa_J04500 on pSB1C3

Electrophoresis

Researcher: Mimata, Kishimoto, Hirao

Part: BBa_K2015011

Electrophoresis

Researcher: Mimata, Hirao

Part: BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3, pSB1C3

Electrophoresis

Researcher: Mimata, Hirao

Part: BBa_J04500 on pSB1C3, BBa_J04500, BBa_K2015011

Digestion

Researcher: Hirao, Mimata

Part: BBa_K2015011

Gel Free System

Researcher: Hirao, Mimata

Part: BBa_J04500, BBa_I13401

Transformation

Researcher: Kishida

Part: BBa_R0010 on pSB1C3, BBa_J04500 on pSB1C3

Liquid Culture

Researcher: Mimata, Shibata

Part: BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3

Digestion

Researcher: Hirao, Mimata

Part: BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3

Electrophoresis

Researcher: Shibata, Hirao, Kishida

Part: pSB1C3, BBa_K2015011, BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3

Ethanol Precipitation

Researcher: Shibata

Part: BBa_J04500, BBa_I13401

Gel Extract

Researcher: Kishida

Part: pSB1C3

Electrophoresis

Researcher: Shibata

Part: pSB1C3, BBa_J04500, BBa_I13401

Digestion

Researcher: Shibata

Part: BBa_J04500 on pSB1C3

Ligation

Researcher: Shibata

Part: pSB1C3 & BBa_K2015011

Gel Free System

Researcher: Mimata

Part: BBa_J04500, BBa_I13401

Ethanol Precipitation

Researcher: Shibata

Part: BBa_J04500, BBa_I13401

Transformation

Researcher: Shibata

Part: BBa_K2015011 on pSB1C3

2016/10/02

Digestion

Researcher: Shibata

Part: BBa_J04500, BBa_I13401

Electrophoresis

Researcher: Shibata

Part: BBa_J04500 on pSB1C3, BBa_J04500, BBa_I13401

PEG Precipitation

Researcher: Shibata

Part: BBa_J04500 on pSB1C3

Electrophoresis

Researcher: Shibata

Part: BBa_J04500 on pSB1C3, BBa_J04500, BBa_I13401

Digestion

Researcher: Shibata

Part: BBa_J04500 on pSB1C3

Digestion

Researcher: Shibata

Part: BBa_K2015009 on pSB1C3, BBa_J04500, BBa_I13401

Mini-prep

Researcher: Shibata

Part: BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3

PEG Precipitation

Researcher: Mimata

Part: BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3

Electrophoresis

Researcher: Mimata, Shibata

Part: BBa_J04500 on pSB1C3, BBa_K2015009 on pSB1C3, BBa_J04500, BBa_I13401, BBa_K2015008 on pSB1C3

Digestion

Researcher: Mimata

Part: BBa_J04500, BBa_I13401, BBa_I13401 on pSB1A2

Electrophoresis

Researcher: Mimata

Part: BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3

Digestion

Researcher: Mimata

Part: BBa_K2015008 on pSB1C3

Transformation

Researcher: Mimata, Hirao

Part: BBa_R0010 on pSB1C3, BBa_J04500 on pSB1C3

Electrophoresis

Researcher: Mimata, Hirao

Part: BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3, BBa_J04500, BBa_I13401, BBa_I13401 on pSB1A2

Electrophoresis

Researcher: Hirao, Fujita

Part: BBa_J04500, BBa_I13401

Gel Extract

Researcher: Hirao, Fujita

Part: BBa_J04500, BBa_I13401

Electrophoresis

Researcher: Hirao, Fujita

Part: BBa_J04500, BBa_I13401

Colony PCR

Researcher: Fujita, Hirao

Part: BBa_K2015011

Ligation

Researcher: Fujita, Hirao

Part: BBa_J04500 & BBa_K2015008 on pSB1C3, BBa_J04500 & BBa_K2015009 on pSB1C3, BBa_J04500 & BBa_I13401 on pSB1A2,

Digestion

Researcher: Fujita, Kishimoto

Part: BBa_I13401, BBa_J04500

Transformation

Researcher: Mimata, Hirao, Fujita, Kishimoto

Part: BBa_J04500 - BBa_I13401 on pSB1A2, BBa_J04500 - BBa_K2015008 on pSB1C3, BBa_J04500 - BBa_K2015009 on pSB1C3

Electrophoresis

Researcher: Kishimoto, Mimata, Fujita

Part: BBa_J04500, BBa_I13401

Electrophoresis

Researcher: Kishimoto, Fujita

Part: BBa_K2015011

Electrophoresis

Researcher: Kono

Part: BBa_J04500, BBa_I13401

Gel Extract

Researcher: Kono

Part: BBa_J04500, BBa_I13401

2016/10/03

Electrophoresis

Researcher: Kono

Part: BBa_J04500, BBa_I13401

Streaking (Single Colony Isolation)

Researcher: Mimata

Part: BBa_J04500 - BBa_I13401 on pSB1A2

Colony PCR

Researcher: Mimata, Fujita

Part: BBa_K2015011

Transformation

Researcher: Mimata, Fujita

Part: BBa_J04500 on pSB1C3

Digestion

Researcher: Mimata, Fujita

Part: BBa_I13401 on pSB1A2

Electrophoresis

Researcher: Fujita

Part: BBa_I13401 on pSB1A2, BBa_K2015011

Colony PCR

Researcher: Mimata, Fujita, Shibata

Part: BBa_J04500 - BBa_K2015009

Digestion

Researcher: Mimata, Fujita, Shibata

Part: BBa_K2015011

Electrophoresis

Researcher: Fujita, Kono

Part: BBa_K2015011

Liquid Culture

Researcher: Shibata

Part: BBa_R0010 on pSB1C3

Ligation

Researcher: Fujita

Part: BBa_K2015011 & pSB1C3, BBa_J04500 & BBa_K2015008 on pSB1C3

Electrophoresis

Researcher: Fujita, Kono

Part: BBa_J04500 - BBa_K2015009

Transformation

Researcher: Fujita

Part: BBa_K2015011 on pSB1C3, BBa_J04500 - BBa_K2015008 on pSB1C3

Ligation

Researcher: Fujita

Part: BBa_J04500 & BBa_K2015008 on pSB1C3, BBa_J04500 & BBa_K2015009 on pSB1C3

2016/10/04

Streaking (Single Colony Isolation)

Researcher: Fujita

Part: BBa_J04500 - BBa_I13401 on pSB1A2

Mini-prep

Researcher: Fujita

Part: BBa_R0010 on pSB1C3

Electrophoresis

Researcher: Fujita

Part: BBa_R0010 on pSB1C3

PEG Precipitation

Researcher: Fujita, Mimata

Part: BBa_R0010 on pSB1C3

Transformation

Researcher: Fujita, Mimata, Shibata

Part: BBa_J04500 - BBa_K2015008 on pSB1C3, BBa_J04500 - BBa_K2015009 on pSB1C3

Colony PCR

Researcher: Mimata, Shibata

Part: BBa_K2015011, BBa_J04500 - BBa_K2015008

Electrophoresis

Researcher: Mimata, Shibata

Part: BBa_R0010 on pSB1C3

PCR (2 STEP)

Researcher: Mimata, Shibata

Part: BBa_J04500

PEG Precipitation

Researcher: Mimata, Shibata

Part: BBa_I13401 on pSB1A2

Electrophoresis

Researcher: Mimata, Shibata

Part: BBa_R0010 on pSB1C3, BBa_I13401 on pSB1A2

Electrophoresis

Researcher: Mimata, Shibata

Part: BBa_J04500

Electrophoresis

Researcher: Mimata

Part: BBa_K2015011

Electrophoresis

Researcher: Shibata

Part: BBa_J04500 - BBa_K2015008

Colony PCR

Researcher: Fujita, Kono, Kishida

Part: BBa_K2015011

Gel Extract

Researcher: Shibata

Part: BBa_J04500

Electrophoresis

Researcher: Shibata

Part: BBa_J04500

Digestion

Researcher: Fujita, Kishida

Part: BBa_I13401 on pSB1A2, BBa_J04500

Electrophoresis

Researcher: Shibata

Part: BBa_I13401 on pSB1A2, BBa_J04500

Electrophoresis

Researcher: Mimata, Fujita

Part: BBa_K2015011

Ligation

Researcher: Mimata, Fujita, Kono

Part: BBa_I13401 on pSB1A2 & BBa_J04500

Streaking (Single Colony Isolation)

Researcher: Kishida, Mimata

Part: BBa_J04500 - BBa_I13401 on pSB1A2

2016/10/05

Colony PCR

Researcher: Mimata

Part: BBa_K2015011

Transformation

Researcher: Mimata, Shibata

Part: BBa_J04500 - BBa_I13401 on pSB1A2, BBa_J04500 on pSB1C3

Liquid Culture

Researcher: Mimata, Shibata

Part: BBa_J04500 on pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa_K2015011

Colony PCR

Researcher: Mimata, Shibata

Part: BBa_K2015011

PCR (2 STEP)

Researcher: Mimata, Shibata

Part: BBa_E0040

Transformation

Researcher: Mimata, Shibata

Part: BBa_B0034 on pSB1A2

Liquid Culture

Researcher: Mimata, Shibata

Part: BBa_B0034 on pSB1A2

Electrophoresis

Researcher: Shibata

Part: BBa_K2015011, BBa_E0040

2016/10/06

Mini-prep

Researcher: Mimata, Shibata

Part: BBa_J04500 on pSB1C3, BBa_I13401 on pSB1A2

Electrophoresis

Researcher: Mimata

Part: BBa_J04500 on pSB1C3, BBa_I13401 on pSB1A2

PEG Precipitation

Researcher: Mimata

Part: BBa_J04500 on pSB1C3, BBa_I13401 on pSB1A2

Electrophoresis

Researcher: Mimata

Part: BBa_J04500 on pSB1C3, BBa_I13401 on pSB1A2

PCR (2 STEP)

Researcher: Nishimura, Mimata

Part: BBa_K2015011, BBa_J04500, BBa_I13401

Electrophoresis

Researcher: Mimata, Fujita

Part: BBa_K2015011

PCR (2 STEP)

Researcher: Fujita, Mimata, Hirao

Part: BBa_K2015011, BBa_J04500

Gel Extract

Researcher: Hirao

Part: BBa_I13401

Electrophoresis

Researcher: Hirao, Fujita

Part: BBa_K2015011 on pSB1C3, BBa_I13401

Liquid Culture

Researcher: Mimata, Fujita, Hirao

Part: BBa_K2015000 on pSB1C3, BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3

Gel Extract

Researcher: Mimata

Part: BBa_K2015011 on pSB1C3

Electrophoresis

Researcher: Fujita

Part: BBa_J04500

2016/10/07

Gel Extract

Researcher: Nishimura

Part: BBa_J04500

Electrophoresis

Researcher: Nishimura

Part: BBa_J04500, BBa_K2015011 on pSB1C3

Liquid Culture

Researcher: Nishimura

Part: BBa_J04500 - BBa_I13401 on pSB1A2

Digestion

Researcher: Nishimura

Part: BBa_K2015011

Ligation

Researcher: Kishida, Fujita

Part: BBa_K2015011

Mini-prep

Researcher: Mimata, Shibata

Part: BBa_K2015000 on pSB1C3, BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3

Electrophoresis

Researcher: Mimata, Shibata

Part: BBa_K2015000 on pSB1C3, BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3

PEG Precipitation

Researcher: Mimata, Shibata

Part: BBa_K2015000 on pSB1C3, BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3

Electrophoresis

Researcher: Shibata

Part: BBa_K2015011

Digestion

Researcher: Fujita, Kishida, Shibata, Mimata

Part: BBa_J04500 on pSB1C3, BBa_K2015008, BBa_K2015009, BBa_I13401

Liquid Culture

Researcher: Nishimura

Part: BBa_B0034 on pSB1A2, BBa_J04500 - BBa_I13401 on pSB1A2

Streaking (Single Colony Isolation)

Researcher: Nishimura

Part: BBa_B0034 on pSB1A2, BBa_J04500 - BBa_I13401 on pSB1A2

Electrophoresis

Researcher: Fujita

Part: BBa_K2015000 on pSB1C3, BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3, BBa_J04500 on pSB1C3, BBa_K2015008, BBa_K2015009, BBa_I13401

Transformation

Researcher: Fujita, Kishida, Nishimura

Part: BBa_K2015011

Ethanol Precipitation

Researcher: Fujita

Part: BBa_K2015000 on pSB1C3, BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3

Ligation

Researcher: Shibata, Fujita, Kishida

Part: BBa_J04500 on pSB1C3 & BBa_K2015008, BBa_J04500 on pSB1C3 & BBa_K2015009, BBa_J04500 on pSB1C3 & BBa_I13401

Transformation

Researcher: Fujita

Part: BBa_J04500 - BBa_K2015008 on pSB1C3, BBa_J04500 - BBa_K2015009 on pSB1C3, BBa_J04500 - BBa_I13401on pSB1C3

2016/10/08

Liquid Culture

Researcher: Nishimura

Part: BBa_J04500 - BBa_I13401 on pSB1A2

Mini-prep

Researcher: Nishimura

Part: BBa_B0034 on pSB1A2

PEG Precipitation

Researcher: Nishimura

Part: BBa_B0034 on pSB1A2

Electrophoresis

Researcher: Nishimura

Part: BBa_B0034 on pSB1A2

Colony PCR

Researcher: Shibata, Nishimura, Kishida

Part: BBa_K2015011

PCR (3 STEP)

Researcher: Shibata

Part: BBa_J04500

Transformation

Researcher: Kishida

Part: BBa_J04500 on pSB1C3

PEG Precipitation

Researcher: Shibata

Part: BBa_J04500 on pSB1C3

Electrophoresis

Researcher: Kishida

Part: BBa_K2015011

Electrophoresis

Researcher: Shibata

Part: BBa_J04500

Digestion

Researcher: Shibata

Part: BBa_J04500 on pSB1C3

Electrophoresis

Researcher: Shibata

Part: BBa_J04500 on pSB1C3

Digestion

Researcher: Mimata, Shibata

Part: BBa_J04500 on pSB1C3

Ethanol Precipitation

Researcher: Mimata, Shibata

Part: BBa_J04500 on pSB1C3

Liquid Culture

Researcher: Mimata, Shibata

Part: BBa_J04500 - BBa_I13401 on pSB1C3, BBa_J04500 - BBa_K2015009 on pSB1C3

PCR (2 STEP)

Researcher: Mimata, Shibata

Part: BBa_R0010, BBa_B0034

Ethanol Precipitation

Researcher: Mimata, Shibata

Part: BBa_J04500 on pSB1C3, BBa_R0010, BBa_B0034

2016/10/09

Mini-prep

Researcher: Nishimura, Hirao

Part: BBa_J04500 - BBa_K2015009 on pSB1C3, BBa_J04500 on pSB1C3, BBa_J04500 - BBa_I13401 on pSB1C3, BBa_B0034 on pSB1A2

Electrophoresis

Researcher: Mimata

Part: BBa_J04500 - BBa_K2015009 on pSB1C3, BBa_J04500 on pSB1C3, BBa_J04500 - BBa_I13401 on pSB1C3, BBa_B0034 on pSB1A2

Colony PCR

Researcher: Nishimura

Part: BBa_K2015011

Digestion

Researcher: Mimata

Part: BBa_J04500 on pSB1C3

PEG Precipitation

Researcher: Mimata, Nishimura, Hirao

Part: BBa_J04500 on pSB1C3, BBa_B0034 on pSB1A2

Digestion

Researcher: Mimata

Part: BBa_J04500 on pSB1C3

PCR (2 STEP)

Researcher: Hirao, Kono

Part: BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3

Electrophoresis

Researcher: Hirao, Nishimura

Part: BBa_J04500 on pSB1C3, BBa_J04500, BBa_B0034

Electrophoresis

Researcher: Hirao

Part: BBa_K2015011

Transformation

Researcher: Mimata, Shibata

Part: BBa_I715039 on pSB1C3, BBa_K741002 on pSB1C3, BBa_J04450 on pSB1C3

Electrophoresis

Researcher: Hirao, Kono

Part: BBa_K2015008, BBa_K2015009

PCR (2 STEP)

Researcher: Nishimura

Part: BBa_R0010 - BBa_B0034

Electrophoresis

Researcher: Kono

Part: BBa_K2015008, BBa_K2015009

Gel Extract

Researcher: Kono

Part: BBa_K2015008, BBa_K2015009

Liquid Culture

Researcher: Shibata

Part: BBa_K2015011 on pSB1C3

Electrophoresis

Researcher: Fujita, Mimata, Kono

Part: BBa_K2015008, BBa_K2015009, BBa_R0010 - BBa_B0034

2016/10/10

Mini-prep

Researcher: Fujita

Part: BBa_K2015011 on pSB1C3

PEG Precipitation

Researcher: Fujita

Part: BBa_K2015011 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015011 on pSB1C3

Digestion

Researcher: Fujita

Part: BBa_K2015011 on pSB1C3

Electrophoresis

Researcher: Fujita

Part: BBa_K2015011 on pSB1C3

Digestion

Researcher: Nishimura

Part: BBa_R0010, BBa_B0034

Transformation

Researcher: Nishimura

Part: BBa_K741002 on pSB1C3, BBa_J04450 on pSB1C3

Electrophoresis

Researcher: Nishimura, Shibata

Part: BBa_R0010, BBa_B0034

Electrophoresis

Researcher: Nishimura, Shibata

Part: BBa_R0010

Gel Extract

Researcher: Nishimura, Shibata

Part: BBa_R0010

Ethanol Precipitation

Researcher: Nishimura

Part: BBa_B0034

Electrophoresis

Researcher: Hirao

Part: BBa_R0010, BBa_B0034

Digestion

Researcher: Shibata, Hirao

Part: BBa_R0010 on pSB1C3, BBa_B0034 on pSB1A2

Ligation

Researcher: Nishimura

Part: BBa_R0010 & BBa_B0034 on pSB1C3

Transformation

Researcher: Nishimura

Part: BBa_R0010 - BBa_B0034 on pSB1A2

2016/10/11

PCR (2 STEP)

Researcher: Nishimura

Part: BBa_R0010 - BBa_B0034

PEG Precipitation

Researcher: Nishimura

Part: BBa_R0010 on pSB1C3

Liquid Culture

Researcher: Nishimura

Part: BBa_R0010 on pSB1C3

Transformation

Researcher: Nishimura

Part: BBa_K880005 on pSB1C3

Ligation

Researcher: Shibata

Part: BBa_R0010 & BBa_B0034 on pSB1A2

Transformation

Researcher: Fujita

Part: BBa_R0010 - BBa_B0034 on pSB1A2

Mini-prep

Researcher: Mimata

Part: BBa_R0010 on pSB1C3, BBa_B0034 on pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa_R0010 - BBa_B0034, BBa_R0010 on pSB1C3, BBa_B0034 on pSB1A2

Transformation

Researcher: Mimata

Part: BBa_K741002 on pSB1C3

PEG Precipitation

Researcher: Mimata, Kishida

Part: BBa_R0010 on pSB1C3, BBa_B0034 on pSB1A2

Electrophoresis

Researcher: Mimata, Kono, Kishida

Part: BBa_R0010 on pSB1C3, BBa_B0034 on pSB1A2

2016/10/12

Liquid Culture

Researcher: Shibata

Part: BBa_K741002 on pSB1C3

Colony PCR

Researcher: Shibata, Mimata, Kishida

Part: BBa_K2015011

Transformation

Researcher: Nishimura

Part: pET15b

Liquid Culture

Researcher: Shibata, Mimata

Part: BBa_K2015011 on pSB1C3, BBa_K2015012 on pSB1C3

Electrophoresis

Researcher: Kishimoto

Part: BBa_K2015011

2016/10/13

PCR (3 STEP)

Researcher: Nishimura

Part: BBa_K2015012

Mini-prep

Researcher: Nishimura, Kishimoto

Part: BBa_K2015011 on pSB1C3

Electrophoresis

Researcher: Shibata

Part: BBa_K2015012 on pSB1A2, BBa_E0040

PEG Precipitation

Researcher: Shibata, Nishimura

Part: BBa_K741002 on pSB1C3

Electrophoresis

Researcher: Nishimura, Hirao

Part: BBa_K2015012 on pSB1A2, BBa_E0040

Gel Extract

Researcher: Nishimura, Hirao

Part: BBa_K2015012 on pSB1A2, BBa_E0040

Mini-prep

Researcher: Kono, Nishimura

Part: BBa_K2015012 on pSB1A2, BBa_K2015012 on pSB1C3

PCR (3 STEP)

Researcher: Nishimura

Part: BBa_K2015012 on pSB1A2

Transformation

Researcher: Nishimura

Part: BBa_E0040 on pSB1A2, BBa_J04450 on pSB1C3

Electrophoresis

Researcher: Kishida

Part: BBa_K741002 on pSB1C3

Liquid Culture

Researcher: Kishida

Part: pET15b

Electrophoresis

Researcher: Kishida, Kono

Part: BBa_K2015012 on pSB1A2

2016/10/14

Digestion

Researcher: Nishumura

Part: BBa_K2015012, BBa_K2015008, BBa_K2015009

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015012 on pSB1A2

Ethanol Precipitation

Researcher: Nishimura

Part: BBa_K2015012

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015012 on pSB1A2, BBa_K2015008, BBa_K2015009

Mini-prep

Researcher: Kono, Mimata

Part: pET15b

Electrophoresis

Researcher: Kono, Mimata

Part: pET15b

PEG Precipitation

Researcher: Kono, Mimata

Part: pET15b

PCR (2 STEP)

Researcher: Mimata

Part: BBa_K2015008, BBa_K2015009

Electrophoresis

Researcher: Mimata

Part: pET15b, BBa_K2015012, BBa_K2015008, BBa_K2015009

Ligation

Researcher: Fujita

Part: BBa_K2015012 & BBa_K2015008 on pSB1C3, BBa_K2015012 & BBa_K2015009 on pSB1C3

Digestion

Researcher: Mimata, Fujita, Shibata, Kono

Part: pET15b, BBa_K2015008, BBa_K2015009

Transformation

Researcher: Fujita

Part: BBa_K2015012 - BBa_K2015008 on pSB1C3, BBa_K2015012 - BBa_K2015009 on pSB1C3

Streaking (Single Colony Isolation)

Researcher: Kono

Part: BBa_E0040 on pSB1A2

Ethanol Precipitation

Researcher: Mimata, Fujita

Part: pET15b, BBa_K2015008, BBa_K2015009

Electrophoresis

Researcher: Mimata, Fujita

Part: pET15b, BBa_K2015008, BBa_K2015009, BBa_K2015011 on pSB1C3

PEG Precipitation

Researcher: Fujita

Part: BBa_K2015011 on pSB1C3

2016/10/15

Digestion

Researcher: Nishimura

Part: pET15b

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015008, BBa_K2015009

Gel Extract

Researcher: Nishimura

Part: BBa_K2015008, BBa_K2015009

Digestion

Researcher: Nishimura

Part: BBa_K2015012 on pSB1A2

Electrophoresis

Researcher: Fujita, Shibata

Part: BBa_K2015011 on pSB1C3

Ethanol Precipitation

Researcher: Nihsimura, Fujita

Part: pET15b

Electrophoresis

Researcher: Nishimura

Part: pET15b, BBa_K2015008, BBa_K2015009, BBa_E0040, BBa_K2015012

Digestion

Researcher: Nishimura

Part: pET15b

Ethanol Precipitation

Researcher: Nihsimura

Part: pET15b

Digestion

Researcher: Nishimura

Part: BBa_K2015009 on pSB1C3

PEG Precipitation

Researcher: Nishimura

Part: pET15b

Electrophoresis

Researcher: Nishimura

Part: pET15b, BBa_K2015009 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: pET15b

Digestion

Researcher: Nishimura

Part: BBa_K2015000 on pSB1C3

Digestion

Researcher: Nishimura

Part: pET15b

Electrophoresis

Researcher: Nishimura

Part: pET15b, BBa_K2015000 on pSB1C3

Streaking (Single Colony Isolation)

Researcher: Nishimura

Part: BBa_E0040 on pSB1A2

Digestion

Researcher: Nishimura

Part: BBa_E0040 on pSB1A2

Colony PCR

Researcher: Nishimura

Part: BBa_K2015012 - BBa_K2015009

Digestion

Researcher: Nishimura

Part: BBa_K2015000 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa_E0040 on pSB1A2

Liquid Culture

Researcher: Nishimura

Part: BBa_K2015012 - BBa_K2015009 on pSB1C3

Digestion

Researcher: Nishimura

Part: pET15b

Electrophoresis

Researcher: Nishimura

Part: pET15b, BBa_K2015000 on pSB1C3

Ligation

Researcher: Nishimura

Part: BBa_K2015012 & BBa_K2015008 on pSB1C3

Digestion

Researcher: Nishimura

Part: BBa_K2015012 on pSB1A2, BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015012 on pSB1A2, BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3

Electrophoresis

Researcher: Hirao

Part: BBa_K2015012 - BBa_K2015009

PCR (3 STEP)

Researcher: Nishimura

Part: BBa_K2015012, BBa_E0040

Transformation

Researcher: Nishimura, Mimata

Part: BBa_K2015012 - BBa_K2015008 on pSB1C3

Mini-prep

Researcher: Hirao

Part: pET15b

PEG Precipitation

Researcher: Hirao, Mimata

Part: pET15b

Electrophoresis

Researcher: Mimata

Part: BBa_K2015012 on pSB1A2, BBa_K2015008 on pSB1C3, BBa_K2015009 on pSB1C3

Liquid Culture

Researcher: Mimata

Part: BBa_K2015012 - BBa_K2015009 on pSB1C3

PCR (2 STEP)

Researcher: Mimata

Part: BBa_K741002

Ligation

Researcher: Mimata

Part: BBa_K2015012 on pSB1A2 & BBa_K2015008

Electrophoresis

Researcher: Mimata

Part: BBa_K2015012 on pSB1A2, BBa_K2015012, BBa_E0040, BBa_K741002, BBa_R0010 - BBa_B0034

2016/10/16

Transformation

Researcher: Fujita, nishimura

Part: BBa_K2015012 - BBa_K2015008 on pSB1C3

Electrophoresis

Researcher: Fujita

Part: BBa_K2015012 on pSB1C3

Gel Extract

Researcher: Fujita

Part: BBa_K2015012 on pSB1C3

Mini-prep

Researcher: Nishimura

Part: BBa_K2015012 - BBa_K2015009 on pSB1C3

PEG Precipitation

Researcher: Nishimura

Part: BBa_K2015012 - BBa_K2015009 on pSB1C3

PCR (3 STEP)

Researcher: Mimata, Nishimura

Part: BBa_K741002, BBa_E0040

Transformation

Researcher: Mimata

Part: BBa_E0040 on pSB1A2

Digestion

Researcher: Nishimura, Mimata

Part: pET15b, BBa_K2015011 on pSB1C3, BBa_K741002

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015012 on pSB1A2, BBa_K2015012 - BBa_K2015009 on pSB1C3, BBa_K741002, BBa_E0040, pET15b

Digestion

Researcher: Nishimura, Mimata

Part: pET15b, BBa_K2015011 on pSB1C3

Liquid Culture

Researcher: Mimata, Shibata

Part: BBa_K741002 on pSB1C3, BBa_E0040 on pSB1A2, pET15b

Electrophoresis

Researcher: Mimata, Shibata

Part: BBa_K741002 on pSB1C3

Gel Extract

Researcher: Shibata, Hirao

Part:BBa_K741002

Electrophoresis

Researcher: Mimata, Shibata

Part: BBa_K741002

2016/10/17

Digestion

Researcher: Nishimura, Shibata

Part: pET15b, BBa_K2015011 on pSB1C3

Liquid Culture

Researcher: Fujita

Part: BBa_K2015012 - BBa_K2015009 on pSB1C3

Colony PCR

Researcher: Fujita

Part: BBa_K2015012 - BBa_K2015008

Liquid Culture

Researcher: Fujita

Part: BBa_K2015012 - BBa_K2015008 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015011 on pSB1C3, pET15b

Ligation

Researcher: Nishimura

Part: BBa_K2015011 & BBa_K741002 on pSB1C3

Digestion

Researcher: Nishimura

Part: pET15b

Electrophoresis

Researcher: Nishimura

Part: BBa_K2015008, BBa_K2015009, pET15b

Transformation

Researcher: Fujita, Nishimura

Part: BBa_K2015011 - BBa_K741002 on pSB1C3

Digestion

Researcher: Fujita

Part: BBa_K2015008, BBa_K2015009

Electrophoresis

Researcher: Fujita

Part: BBa_K2015012 - BBa_K2015008, BBa_K2015011 - BBa_K741002 on pSB1C3

Electrophoresis

Researcher: Fujita, Nishimura

Part: pET15b, BBa_K2015008, BBa_K2015009

Liquid Culture

Researcher: Nishimura

Part: BBa_K2015011 on pSB1C3

Mini-prep

Researcher: Kishida

Part: pET15b, BBa_K741002 on pSB1C3

PEG Precipitation

Researcher: Nishimura

Part: pET15b, BBa_K741002 on pSB1C3

Transformation

Researcher: Nishimura

Part: BBa_K2015008 on pET15b, BBa_K2015009 on pET15b

Ethanol Precipitation

Researcher: Nishimura

Part: BBa_K741002

Mini-prep

Researcher: Kishida

Part: BBa_K2015012 - BBa_K2015008 on pSB1C3

Electrophoresis

Researcher: Kishida

Part: BBa_K2015012 - BBa_K2015008 on pSB1C3, pET15b, BBa_K741002 on pSB1C3

Ligation

Researcher: Nishimura

Part: BBa_K2015008 & pET15b, BBa_K2015009 & pET15b

Transformation

Researcher: Nishimura

Part: BBa_K2015008 on pET15b, BBa_K2015009 on pET15b

Liquid Culture

Researcher: Mimata, Hirao

Part: BBa_K2015011 - BBa_K741002 on pSB1C3

2016/10/18

Liquid Culture

Researcher: Mimata, Hirao

Part: BBa_K2015011 - BBa_K741002 on pSB1C3

Colony PCR

Researcher: Nishimura

Part: BBa_K2015011 - BBa_K741002 on pET15b

Mini-prep

Researcher: Kono

Part: BBa_K2015011 - BBa_K741002 on pSB1C3

PEG Precipitation

Researcher: Kono

Part: BBa_K2015011 - BBa_K741002 on pSB1C3

Electrophoresis

Researcher: Kishida

Part: BBa_K2015011 - BBa_K741002 on pSB1C3

Electrophoresis

Researcher: Kono

Part: BBa_K2015011 - BBa_K741002 on pSB1C3

2016/10/19

Colony PCR

Researcher: Nishimura, Shibata

Part: BBa_K2015011 - BBa_K741002 on pSB1C3

Ligation

Researcher: Nishimura

Part: BBa_K2015008 & BBa_K2015009 on pET15b