Team:Michigan/Results
Results
title 1
1) Rehydrated DNA from distribution kit
2) Transformed into DH5a chemically competent cells
3) Extracted plasmid
4) PCR mutagenesis to engineer desired cut sites around LacZ
Proximity Ligation Assay in an agarose gel using sybr
Caption
Creation of delta-M15 mutant
1) Deletion of segment including alpha fragment of LacZ using NEB Q5 site directed mutagenesis kit
2) Submitted for sequencing to confirm successful deletion
Proximity dependent ligation assay
1) Probe segments and bridge segments mixed with T4 ligase in presence or absence of thrombin
2) Gel electropheresis to detect ligation
Cloned delta-M15 mutant into submission vector
1) Digested linearized pSB1C3 with our mutant
2) Ligated together
3) Transformed into new DH5a chemically competent cells
5) Extracted DNA
6) Dried, packaged, shipped
3) Transformed into new DH5a chemically competent cells
