Results

Isolated LacZ from iGem part BBa_K564012

CAPTION HERE

Transferring LacZ into pET28

In order to have a control for our Cell Free expression system we cloned LacZ into the protein expression plasmid to compare LacZ expression to expression of our system with and without the presence of its target protein.

Creation of delta-M15 Mutant

Successfully deleted the alpha fragment in our Lacz plasmid, leaving only the omega fragment. The omega fragment complements the alpha fragment produced in the proximity-dependent ligation forming functional LacZ. This enzyme can break down X-Gal giving a colorimetric output. The plasmid is a very important part of our system, allowing alpha complementation and therefore producing a color visible to the naked eye.

Also, click here to see sequencing results.

Proximity Ligation Assay in Agarose Gel using SYBR