Difference between revisions of "Team:HokkaidoU Japan/Aggregation"
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| − | <br> When you harvest <span style="font-style: italic">E. coli</span> on your laboratory, is there | + | <br> When you harvest <span style="font-style: italic">E. coli</span> on your laboratory, is there a efficiently way to aggregate <span style="font-style: italic">E. coli</span> with the SAP? We would like to try to suggest a possibility of the expression of <span style="font-style: italic">E. coli</span> aggregation by expression of the SAP fused to <span style="font-style: italic">E. coli</span> flagellum. Perhaps, a new method for the <span style="font-style: italic">E. coli</span> aggregation, except for Ag43 which has been adopted by some iGEM teams[1][2], may be found. |
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Revision as of 19:44, 19 October 2016
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Team:HokkaidoU Japan
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When you harvest E. coli on your laboratory, is there a efficiently way to aggregate E. coli with the SAP? We would like to try to suggest a possibility of the expression of E. coli aggregation by expression of the SAP fused to E. coli flagellum. Perhaps, a new method for the E. coli aggregation, except for Ag43 which has been adopted by some iGEM teams[1][2], may be found.
FliC is an important domain which forms the filament (Fig. 2)[3]. It is known that any protein can be inserted into its domain without losing the function of the flagella. So we think that the aggregation of E. coli because of the bindings between the flagella is likely to happen by inserting SAP, for example, RADA16-I and P11-4. Since it has been reported that the overexpression of the Ag43 decreases the survival rate of E. coli, we have to search for an alternative method for the aggregation of E. coli.
Therefore we propose that inserting the SAP has an advantage of improving the efficiency of the consecutive chemical reactions when E. coli which have different functions approach each other.
There may still be room for improvement for the financial aspect or the efficiency regarding the way to harvest E. coli in the laboratory. In addition, a simpler method would be much more desirable without any expensive biological device. At last we wish that more research will be conducted of the difference between the efficiency of the aggregation of E. coli with the biological tools, for example Ag43, RADA16-I, and P11-4. And also we desire for the possibility of the aggregation of E. coli with the SAP we suggest.
[1] HokkaidoU Japan 2012
[2] Aberdeen Scotland 2014
[3] Kuwajima, G. 1988. Construction of a minimum-size functional flagellin of Escherichia coli. J. Bacteriol. 170:3305-3309.
When you harvest E. coli on your laboratory, is there a efficiently way to aggregate E. coli with the SAP? We would like to try to suggest a possibility of the expression of E. coli aggregation by expression of the SAP fused to E. coli flagellum. Perhaps, a new method for the E. coli aggregation, except for Ag43 which has been adopted by some iGEM teams[1][2], may be found.
 |
| Fig. 1. Image of aggregation using SAP
E. coli are aggregated by the binding through SAP. SAP is expressed on the fllagerum. |
FliC is an important domain which forms the filament (Fig. 2)[3]. It is known that any protein can be inserted into its domain without losing the function of the flagella. So we think that the aggregation of E. coli because of the bindings between the flagella is likely to happen by inserting SAP, for example, RADA16-I and P11-4. Since it has been reported that the overexpression of the Ag43 decreases the survival rate of E. coli, we have to search for an alternative method for the aggregation of E. coli.
Therefore we propose that inserting the SAP has an advantage of improving the efficiency of the consecutive chemical reactions when E. coli which have different functions approach each other.
 |
| Fig. 2. Image of filament of E. coli
Any protein can be inserted into the FliC. |
There may still be room for improvement for the financial aspect or the efficiency regarding the way to harvest E. coli in the laboratory. In addition, a simpler method would be much more desirable without any expensive biological device. At last we wish that more research will be conducted of the difference between the efficiency of the aggregation of E. coli with the biological tools, for example Ag43, RADA16-I, and P11-4. And also we desire for the possibility of the aggregation of E. coli with the SAP we suggest.
[1] HokkaidoU Japan 2012
[2] Aberdeen Scotland 2014
[3] Kuwajima, G. 1988. Construction of a minimum-size functional flagellin of Escherichia coli. J. Bacteriol. 170:3305-3309.
