Difference between revisions of "Team:HokkaidoU Japan/Kill switch"
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<p>We came up with an idea of utilizing SAP for cell death system. To be specific, we expect SAP which is excessively accumulated inside of E.coli would be vital damage for E.coli by disturbing its metabolism or catabolism, for example. In addition, this system is expected to cause cell death without cell lysis, which is a desirable feature when you want to handle E.coli containing harmful substances like heavy metal ion inside.</p> | <p>We came up with an idea of utilizing SAP for cell death system. To be specific, we expect SAP which is excessively accumulated inside of E.coli would be vital damage for E.coli by disturbing its metabolism or catabolism, for example. In addition, this system is expected to cause cell death without cell lysis, which is a desirable feature when you want to handle E.coli containing harmful substances like heavy metal ion inside.</p> | ||
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<div id="Methods"><img src="https://static.igem.org/mediawiki/2016/2/2c/T--HokkaidoU_Japan--methods.png" | <div id="Methods"><img src="https://static.igem.org/mediawiki/2016/2/2c/T--HokkaidoU_Japan--methods.png" | ||
width="270px" height="90px" alt="methods"></div> | width="270px" height="90px" alt="methods"></div> | ||
<p>For our new cell death system, we designed a construct shown in fig.1. The construct has a lactose promoter upstream of RBS and SAP coding region, thus we can control the timing of switching-on the device. We ordered the parts of DNA from IDT, and after subcloning, we put the piece of DNA on pSB1C3 vector and transformed it into E.coli.(ここもうちょっと丁寧に説明したほうが良い？)</p> | <p>For our new cell death system, we designed a construct shown in fig.1. The construct has a lactose promoter upstream of RBS and SAP coding region, thus we can control the timing of switching-on the device. We ordered the parts of DNA from IDT, and after subcloning, we put the piece of DNA on pSB1C3 vector and transformed it into E.coli.(ここもうちょっと丁寧に説明したほうが良い？)</p> | ||
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| + | <p><div id="Reference"><img src="https://static.igem.org/mediawiki/2016/8/86/T--HokkaidoU_Japan--reference.png" | ||
| + | width="270px" height="90px" alt="reference"></div></p> | ||
| + | <br>ここに本文<br> | ||
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Revision as of 06:04, 16 October 2016
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Team:HokkaidoU Japan
We came up with an idea of utilizing SAP for cell death system. To be specific, we expect SAP which is excessively accumulated inside of E.coli would be vital damage for E.coli by disturbing its metabolism or catabolism, for example. In addition, this system is expected to cause cell death without cell lysis, which is a desirable feature when you want to handle E.coli containing harmful substances like heavy metal ion inside.
For our new cell death system, we designed a construct shown in fig.1. The construct has a lactose promoter upstream of RBS and SAP coding region, thus we can control the timing of switching-on the device. We ordered the parts of DNA from IDT, and after subcloning, we put the piece of DNA on pSB1C3 vector and transformed it into E.coli.(ここもうちょっと丁寧に説明したほうが良い？)
ここに本文
