Team:HokkaidoU Japan/Notebook

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Team:HokkaidoU Japan

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• Top
• Project
  • Overview
    • Project Description
    • Background
    • Proof of concept
    • Modeling
    • Achievements
  • MULTIMERIZATION
    • Overview
    • Methods
    • Results
    • Conclusion
  • FUTURE WORK
    • Circularization
    • Kill Switch
    • Transport
    • Aggregation
  • Extra Materials
    • Parts
    • Notebook
    • Protocols
    • Safety
• Human practices
• Collaborations
• Team
  • Attribution
  • Member

Protocol

August 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

September 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Octorber 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

2016/08/07

Transformation

Researcher: Fujita

Part: BBa_B0034 on pSB1A2, BBa_E0040 on pSB1A2 BBa_R0010 on pSB1C3, BBa_B0015 on pSB1C3, BBa_J04500 on pSB1C3, BBa_E0020 on pSB1C3, BBa_E0030 on pSB1C3

Liquid Culture

Researcher: Fujita, Kishida, Kono

Part: BBa_E0020 on 1c3, BBa_E0030 on 1c3, BBa_E0040 on 1a2, BBa_R0010 on 1c3

2016/08/09

Transformation

Researcher: Kono, Mimata, Nishimura

Part: BBa_B0034 on pSB1A2, BBa_E0040 on pSB1A2, BBa_R0010 on pSB1C3, BBa_B0015 on pSB1C3, BBa_J04500 on pSB1C3

Mini-prep

Researcher: Fujita, Kishida, Kono

Part: BBa_R0010 on pSB1A2, BBa_E0040 on pSB1A2

Gel Free System

Researcher: Mimata, Shibata, Kono, Nishimura

Part: BBa_R0010, BBa_E0040

Ethanol Precipitation

Researcher: Mimata, Shibata, Kono, Nishimura

Part: BBa_R0010, BBa_E0040

2016/08/10

Liquid Culture

Researcher: Mimata

Part: BBa_B0015 on 1c3, BBa_J04500 on 1c3, BBa_R0010 on 1c3

Transformation

Researcher: Mimata

Part: BBa_B0034 on pSB1A2, BBa_E0040 on pSB1A2

Electrophoresis

Researcher: Kishimoto, Kono, Shibata, Nishimura

Part: BBa_R0010, BBa_E0040

Mini-prep

Researcher: Mimata, Kono

Part: BBa_R0010 on pSB1A2, BBa_B0015 on pSB1C3, BBa_J04500 on pSB1C3

Electrophoresis

Researcher: Mimata, Kono, Nishimura

Part: BBa_R0010 on pSB1A2, BBa_B0015 on pSB1C3, BBa_J04500 on pSB1C3

Liquid Culture

Researcher: Kono, Mimata, Nishimura

Part: BBa_B0034 on 1a2, BBa_E0040 on 1c3

2016/08/11

Transformation

Researcher: Nishimura, Kishida

Part: BBa_B0032 on pSB1C3, BBa_R0011 on pSB1A2

Mini-prep

Researcher: Nishimura, Kishida

Part: BBa_E0040 on pSB1A2, BBa_B0034 on pSB1A2

PEG Precipitation

Researcher: Nishimura, Shibata, Noda

Part: BBa_B0015 on pSB1C3, BBa_R0010 on pSB1C3 , BBa_J04500 on pSB1C3, BBa_E0040 on pSB1A2, BBa_B0034 on pSB1A2

Electrophoresis

Researcher: Shibata, Noda

Part: BBa_B0015 on pSB1C3, BBa_R0010 on pSB1C3 , BBa_J04500 on pSB1C3, BBa_E0040 on pSB1A2, BBa_B0034 on pSB1A2

Gel Free System

Researcher: Kono, Nishimura

Part: BBa_B0015, BBa_R0010, BBa_J04500, BBa_E0040, BBa_B0034

Electrophoresis

Researcher: Nishimura

Part: BBa_B0015, BBa_R0010, BBa_J04500, BBa_E0040, BBa_B0034

Ethanol Precipitation

Researcher: Kono, Nishimura

Part: BBa_B0015, BBa_R0010, BBa_J04500, BBa_E0040, BBa_B0034

Electrophoresis

Researcher: Kono, Nishimura

Part: BBa_B0015, BBa_R0010, BBa_J04500, BBa_E0040, BBa_B0034

2016/08/12

Transformation

Researcher: Nishimura

Part: BBa_C0012 on pSB1C3, BBa_J23101 on BBa_J61002

Liquid Culture

Researcher: Nishimura

Part: BBa_B0032 on 1c3, BBa_R0011 on 1a2

2016/08/13

Mini-prep

Researcher: Fujita

Part: BBa_B0032 on pSB1C3

PEG Precipitation

Researcher: Fujita

Part: BBa_B0032 on pSB1C3

Electrophoresis

Researcher: Fujita, Noda

Part: BBa_B0032 on pSB1C3

Streaking (Single Colony Isolation)

Researcher: Shibata, Fujita

Part: BBa_R0011 on pSB1A2

Liquid Culture

Researcher: Shibata, Noda

Part: BBa_C0012 on 1c3, BBa_B0032 on 1c3, BBa_J23101 on BBa_J61002

2016/08/14

Mini-prep

Researcher: Nishimura, Kishimoto

Part: BBa_C0012 on pSB1C3, BBa_B0032 on pSB1C3, BBa_J23101 on BBa_J61002

PEG Precipitation

Researcher: Nishimura, Kono

Part: BBa_C0012 on pSB1C3, BBa_B0032 on pSB1C3, BBa_J23101 on BBa_J61002

Electrophoresis

Researcher: Nishimura, Kono

Part: BBa_C0012 on pSB1C3, BBa_B0032 on pSB1C3, BBa_J23101 on BBa_J61002

Transformation

Researcher: Nishimura, Kono, Donen

Part: BBa_R0011 on pSB1A2

2016/08/15

Gel Free System

Researcher: Fujita, Esashi

Part: BBa_C0012, BBa_J23101, BBa_B0032

Electrophoresis

Researcher: Kishida, Fujita, Donen, Shibata, Esashi

Part: BBa_C0012, BBa_J23101, BBa_B0032

Ethanol Precipitation

Researcher: Fujita, Kishida, Noda

Part: BBa_C0012, BBa_J23101, BBa_B0032

Liquid Culture

Researcher: Shibata

Part: BBa_R0011 on 1a2

2016/08/16

Electrophoresis

Researcher: Nishimura

Part: BBa_C0012, BBa_J23101, BBa_B0032

Mini-prep

Researcher: Nishimura

Part: BBa_R0011 on pSB1A2

PEG Precipitation

Researcher: Nishimura, Kono

Part: BBa_R0011 on pSB1A2

Electrophoresis

Researcher: Nishimura

Part: BBa_R0011 on pSB1A2

PCR (2 STEP)

Researcher: Esashi, Nishimura, Shibata

Part: BBa_R0011

Electrophoresis

Researcher: Nishimura

Part: BBa_R0011

2016/08/17

Gel Free System

Researcher: Sugishita, Shibata

Part: BBa_R0011

Electrophoresis

Researcher: Sugishita, Shibata, Kishida

Part: BBa_R0011

Gel Free System

Researcher: Sugishita, Kishida, Shibata

Part: BBa_R0011

PEG Precipitation

Researcher: Sugishita, Kishida, Shibata

Part: BBa_R0011

Transformation

Researcher: Nishimura

Part: BBa_I13504 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa_R0011

Transformation

Researcher: Shibata

Part: BBa_I13504 on pSB1C3

2016/08/18

Gel Free System

Researcher: Nishimura

Part: BBa_R0011

Ethanol Precipitation

Researcher:

Part: BBa_R0011

Electrophoresis

Researcher: Nishimura

Part: BBa_R0011

Liquid Culture

Researcher: Nishimura

Part: BBa_I13504 on 1c3

Transformation

Researcher: Kono

Part: BBa_I13504 on pSB1C3

2016/08/19

Mini-prep

Researcher: Shibata, Kishida

Part: BBa_I13504 on pSB1C3

PEG Precipitation

Researcher: Shibata

Part: BBa_I13504 on pSB1C3

Electrophoresis

Researcher: Shibata

Part: BBa_I13504 on pSB1C3

PCR (2 STEP)

Researcher: Nishimura

Part: BBa_I13504

Electrophoresis

Researcher: Shibata, Nishimura

Part: BBa_I13504

PCR (2 STEP)

Researcher: Nishimura, Shibata

Part: BBa_I13504

Electrophoresis

Researcher: Shibata, Nishimura

Part: BBa_I13504

2016/08/20

Gel Free System

Researcher: Fujita, Mimata

Part: BBa_I13504

Ethanol Precipitation

Researcher: Fujita, Mimata

Part: BBa_I13504

2016/08/21

Electrophoresis

Researcher: Nishimura, Mimata, Kishimoto

Part: BBa_I13504

Ligation

Researcher: Kishimoto, Nishimura, Mimata

Part: BBa_I13504 & BBa_R0011 on pSB1A2

Transformation

Researcher: Kishimoto, Kono, Mimata

Part: BBa_I13401 on pSB1A2, BBa_R0011 - BBa_I13504 on pSB1A2

2016/08/22

Colony PCR

Researcher: Mimata, Kono, Kishida, Esashi

Part: BBa_R0011 - BBa_I13504

Liquid Culture

Researcher: Mimata, Kono, Kishida, Esashi

Part: BBa_R0011 - BBa_I13504 on 1a2, BBa_I13401 on 1a2

2016/08/23

Mini-prep

Researcher: Mimata, Fujita

Part: BBa_I13401 on pSB1A2

PEG Precipitation

Researcher: Mimata, Fujita

Part: BBa_I13401 on pSB1A2, BBa_R0011 on pSB1A2

Digestion

Researcher: Mimata, Fujita, Yonezawa

Part: BBa_R0011 on pSB1A2

Gel Free System

Researcher: Mimata, Fujita, Yonezawa

Part: BBa_I13504

Ethanol Precipitation

Researcher: Mimata, Fujita, Yonezawa

Part: BBa_I13504

Electrophoresis

Researcher: Mimata, Fujita, Kishida, Kono

Part: BBa_R0011 on pSB1A2, BBa_I13504

Ligation

Researcher: Mimata, Fujita, Kishida, Kono

Part: BBa_R0011 on pSB1A2 & BBa_I13504

Electrophoresis

Researcher: Mimata, Fujita, Kishida, Kono

Part: BBa_R0011 - BBa_I13504 on pSB1A2

Transformation

Researcher: Mimata, Fujita, Kishida, Kono

Part: BBa_R0011 - BBa_I13504 on pSB1A2

Electrophoresis

Researcher: Mimata, Fujita, Kishida, Kono

Part: BBa_R0011 - BBa_I13504 on pSB1A2

2016/08/24

Colony PCR

Researcher: Fujita, Kono

Part: BBa_R0011 - BBa_I13504

Electrophoresis

Researcher: Fujita, Kono, Shibata, Mimata, Hirao

Part: BBa_R0011 - BBa_I13504

PCR (2 STEP)

Researcher: Kono, Shibata, Fujita

Part: IDT1, IDT2, IDT3, IDT4, IDT5, IDT6, IDT7