Difference between revisions of "Team:HokkaidoU Japan/Proof"

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Revision as of 11:52, 18 October 2016

Team:HokkaidoU Japan - 2016.igem.org

 

Team:HokkaidoU Japan

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Proof of concept

We conducted SDS-PAGE with (BBa_K2015012+BBa_K2015008/ BBa_K2015012+BBa_K2015009) on (pSB1C3)of E.coli(DH5α). At first, we made the Gel for SDS-PAGE, with the following the Table1. What was the most important was to add TEMED ffinally because

Table. 1. Gel assay

Materials Volume
30% Acrilmid 5 mL
Tris-Buffer 2.5 mL
SDS 100 µL
APS 100 µL
TEMED 5 µL
DW 2.295 mL
Total 10 mL