Difference between revisions of "Team:HokkaidoU Japan/Proof"

Line 74: Line 74:
 
<br clear="all">
 
<br clear="all">
 
</div>
 
</div>
 +
<br>
 +
  The following Table2 is about preparing the SDS-PAGE.
 +
 
 +
  <center>
 +
<table style="border-style: none">
 +
<tr align="center">
 +
<td style="border-style: none;">
 +
      <tr>
 +
<td style="border-style:none; float:center"><center><h2>Table. 2. Experimental condition</h2></center></td>
 +
      </tr>
 +
      <tr>
 +
<td style="border-style: none"; align="center">
 +
 +
<table align="center">
 +
  <tr align="center">
 +
<th>Temperature</th>
 +
<th>IPTG</th>
 +
<th>Concentration (M)</th>
 +
<th>Volume (&micro;L)</th>
 +
<th>Time to culture</th>
 +
  </tr>
 +
 
 +
    <tr align="center">
 +
    <td>37&deg;C</td>
 +
    <td>-</td>
 +
<td>-</td>
 +
<td>-</td>
 +
<td>24 h</td>
 +
  </tr>
 +
 
 +
  <tr align="center">
 +
    <td>37&deg;C</td>
 +
    <td>+</td>
 +
<td>0.4</td>
 +
<td>6</td>
 +
<td>24 h</td>
 +
  </tr>
 +
 
 +
    <tr align="center">
 +
    <td>37&deg;C</td>
 +
    <td>+</td>
 +
<td>2</td>
 +
<td>30</td>
 +
<td>24 h</td>
 +
  </tr>
 +
 
 +
    <tr align="center">
 +
    <td>25&deg;C</td>
 +
    <td>-</td>
 +
<td>-</td>
 +
<td>-</td>
 +
<td>16 h</td>
 +
  </tr>
 +
 
 +
    <tr align="center">
 +
    <td>25&deg;C</td>
 +
    <td>+</td>
 +
<td>0.4</td>
 +
<td>6</td>
 +
<td>16 h</td>
 +
  </tr>
 +
 
 +
    <tr align="center">
 +
    <td>25&deg;C</td>
 +
    <td>+</td>
 +
<td>2</td>
 +
<td>30</td>
 +
<td>16 h</td>
 +
  </tr>
 +
 
 +
</table>
  
  
  
 
</html>
 
</html>

Revision as of 12:36, 18 October 2016

Team:HokkaidoU Japan - 2016.igem.org

 

Team:HokkaidoU Japan

\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\


Proof of concept

We conducted SDS-PAGE with (BBa_K2015012+BBa_K2015008/ BBa_K2015012+BBa_K2015009) on （pSB1C3）of E.coli（DH5α）. At first, we made the Gel for SDS-PAGE, with the following the Table1. What was the most important was to add TEMED ffinally because

Table. 1. Gel assay
Materials Volume
30％ Acrilmid 5 mL
Tris-Buffer 2.5 mL
SDS 100 µL
APS 100 µL
TEMED 5 µL
DW 2.295 ｍL
Total 10 ｍL


The following Table2 is about preparing the SDS-PAGE.

Table. 2. Experimental condition
Temperature IPTG Concentration (M) Volume (µL) Time to culture
37°C - - - 24 h
37°C + 0.4 6 24 h
37°C + 2 30 24 h
25°C - - - 16 h
25°C + 0.4 6 16 h
25°C + 2 30 16 h