Difference between revisions of "Team:HokkaidoU Japan/Proof"
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| − | <br>And also we | + | <br>And also we induced <a href="http://parts.igem.org/Part:BBa_K2015012">BBa_K2015012</a> + <a href="http://parts.igem.org/Part:BBa_K2015008">BBa_K2015008</a> / <a href="http://parts.igem.org/Part:BBa_K2015012">BBa_K2015012</a> + <a href="http://parts.igem.org/Part:BBa_K2015009">BBa_K2015009</a> on pSB1C3 of <span style="font-style: italic">E. coli</span> (DH5α). |
| − | + | <a href="http://parts.igem.org/Part:BBa_K2015008">BBa_K2015008</a> consists Self Assembling Regions (SAR, RADA16-I) and GFP. And <a href="http://parts.igem.org/Part:BBa_K2015009">BBa_K2015009</a> this part consists Self Assembling Region (SAR, P<span class="sitatuki">11</span>-4) and GFP. | |
| − | + | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2015008">BBa_K2015008</a> consists Self Assembling Regions (SAR, RADA16-I) and | + | |
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<br> | <br> | ||
| − | The results are shown the below pictures.The fluorescence of GFPs was not detected any condition | + | The results are shown the below pictures. The fluorescence of GFPs was not detected any condition. |
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<table style="border-style: none; width:1px; margin-left:auto; margin-right:auto"> | <table style="border-style: none; width:1px; margin-left:auto; margin-right:auto"> | ||
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| − | <td style="border-style: none"; align="center"><span class="small">Fig. | + | <td style="border-style: none"; align="center"><span class="small">Fig. 2. BBa_K2015012-BBa_K2015008<br>BBa_K2015012-BBa_K2015008 on pSB1C3 vector were induced by IPTG and incubated at 37°C. (-): not induced, (+): 0.5 mM IPTG and (++): 1.0 mM IPTG</span></td> |
</tr> | </tr> | ||
</tr> | </tr> | ||
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| − | <span class="small">Fig. | + | <span class="small">Fig. 3. BBa_K2015012-BBa_K2015009<br>BBa_K2015012-BBa_K2015009 on pSB1C3 vector were induced by IPTG and incubated at 25°C for 24 h (left) <br>and at 37°C for 16 h (right). (-): not induced, (+): 0.5 mM IPTG and (++): 1.0 mM IPTG</span> |
</center> | </center> | ||
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Revision as of 02:47, 20 October 2016
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Team:HokkaidoU Japan
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We made a functonal unit to regulate gene expression. BBa_K2015012 codes constitutive promoter (BBa_J23101), RBS (BBa_B0032), LacI (BBa_C0012), dT (BBa_B0015), PLac (BBa_R0011) and RBS (BBa_B0034). To insert mRFP at the downstream we identified this construct could regulate the expression (Fig. 1).
And also we induced BBa_K2015012 + BBa_K2015008 / BBa_K2015012 + BBa_K2015009 on pSB1C3 of E. coli (DH5α). BBa_K2015008 consists Self Assembling Regions (SAR, RADA16-I) and GFP. And BBa_K2015009 this part consists Self Assembling Region (SAR, P11-4) and GFP.
The results are shown the below pictures. The fluorescence of GFPs was not detected any condition.
Fig. 3. BBa_K2015012-BBa_K2015009
BBa_K2015012-BBa_K2015009 on pSB1C3 vector were induced by IPTG and incubated at 25°C for 24 h (left)
and at 37°C for 16 h (right). (-): not induced, (+): 0.5 mM IPTG and (++): 1.0 mM IPTG
We made a functonal unit to regulate gene expression. BBa_K2015012 codes constitutive promoter (BBa_J23101), RBS (BBa_B0032), LacI (BBa_C0012), dT (BBa_B0015), PLac (BBa_R0011) and RBS (BBa_B0034). To insert mRFP at the downstream we identified this construct could regulate the expression (Fig. 1).
 |
| Fig. 1. Change of expression by IPTG induction left: not induced, right: induced |
And also we induced BBa_K2015012 + BBa_K2015008 / BBa_K2015012 + BBa_K2015009 on pSB1C3 of E. coli (DH5α). BBa_K2015008 consists Self Assembling Regions (SAR, RADA16-I) and GFP. And BBa_K2015009 this part consists Self Assembling Region (SAR, P11-4) and GFP.
The results are shown the below pictures. The fluorescence of GFPs was not detected any condition.
 |
| Fig. 2. BBa_K2015012-BBa_K2015008 BBa_K2015012-BBa_K2015008 on pSB1C3 vector were induced by IPTG and incubated at 37°C. (-): not induced, (+): 0.5 mM IPTG and (++): 1.0 mM IPTG |
|
|
BBa_K2015012-BBa_K2015009 on pSB1C3 vector were induced by IPTG and incubated at 25°C for 24 h (left)
and at 37°C for 16 h (right). (-): not induced, (+): 0.5 mM IPTG and (++): 1.0 mM IPTG
