Team:HokkaidoU Japan
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We conducted SDS-PAGE with (BBa_K2015012+BBa_K2015008/ BBa_K2015012+BBa_K2015009) on (pSB1C3)of E.coli(DH5α). At first, we made the Gel for SDS-PAGE, with the following the Table1. What was the most important was to add TEMED ffinally because
We conducted SDS-PAGE with (BBa_K2015012+BBa_K2015008/ BBa_K2015012+BBa_K2015009) on (pSB1C3)of E.coli(DH5α). At first, we made the Gel for SDS-PAGE, with the following the Table1. What was the most important was to add TEMED ffinally because
Materials | Volume |
---|---|
30% Acrilmid | 5 mL |
Tris-Buffer | 2.5 mL |
SDS | 100 µL |
APS | 100 µL |
TEMED | 5 µL |
DW | 2.295 mL |
Total | 10 mL |