Team:ColumbiaU NYC/Collaborations
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Collaborations
University of Georgia
The University of Georgia iGEM team is continuing their work on developing the archaeal Methanococcus maripaludis as an inexpensive chassis for synthetic biochemical production with a current focus on engineering the ribosomal binding site (RBS). An mCherry reporter is used to measure protein expression. In the team's efforts to standardize fluorescence measurements and collect data, we are helping the UGA iGEM team characterize RBS variants by measuring mCherry fluorescence levels using flow cytometry and performing statistical analysis.
Imperial College London
The Imperial College London iGEM team are working on a co-culture technology that allows for self-regulating artificial cell population ratios through cell communication and regulation. As such, the team is also collecting data on growth conditions in various medias. Here, we contributed to the team's efforts by providing data on Pseudomonas putida growth at different mixture ratios of LB and BHI and determining the growth curve in each mixture at OD 600. This effort also benefitted us by granting us insight into how P. putida containing our construct will fare in these mixed media conditions compared to the wild-type strain.
Genspace
The Genspace iGEM team is a team composed of high-schoolers and citizen scientists working at a community laboratory. As such, access to certain lab equipment is typically limited. As a result, the Columbia iGEM team have worked with the team members to help collect data on their behalf. This year, we aided the Genspace team in their efforts to collect data for the UGA-Georgia team1 and characterize constructs by collecting data on cell growth at OD 700 and collecting mTurquoise2 and mCherry fluorescence.
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