Parts
Composite Parts
Our composite parts can be found here:
Composite Parts- BBa_K2109102 lambda c1 Nanobody GBP3
- BBa_K2109103 Lambda c1 Nanobody scaffold
- BBa_K2109104 Bacteria two hybrid selection system
- BBa_K2109105 Bacteria two hybrid selection system with RNA polymerase alpha and pBAD
- BBa_K2109106 RNA polymerase alpha
- BBa_K2109107 RNA polymerase alpha GFP control
- BBa_K2109109 U1A ribozyme control
- BBa_K2109110 U1A-C75T ribozyme control
Improved Parts
We improved a collection of parts this semester.
- BBa_K2109111 - MS2 expression module containing a modifed MS2 protein to prevent dimerization to allow for higher yields during purification. Additionally a N-Terminal His tag was added and expression is modulated via a T7 promoter and strong RBS.
- BBa_K2109108 - Mazf kill switch construct improved by adding RBS and testing the effects of evolutionary dirft.