We have achieved following experiment results as our PROOF OF CONCEPT.
We have constructed a signal pathway in bacteria for sensing the indicating-molecule Nitric Oxide of Inflammatory Bowel Disease and secreting Epidermal Growth Factors which contribute to the recovery of IBD. The pathway, which is illustrated in Figure A, consists of two sections: Nitric oxide sensing and Epidermal Growth Factor expression.
Click here to view EGF Expression
Click here to view NO Sensor
We have constructed a biofilm-display system. Beneficial molecules are linked to biofilm monomers to assembly outside the cell, forming extracellular matrix (Figure B). This would show the potential value of this display approach.
Click here to view Therapeutic Biofilm
We have constructed a kill switch which inhibits the growth of bacteria under our control (Figure C). This switch ensures the safety for both our lab work and the therapy.
Click here to view Kill Switch