Team:Wageningen UR/Notebook
Find the documentation of our research here.
-
Protein Engineering
We wanted to engineer Cry3Aa to become toxic to Varroa destructor.
-
In Vitro Assay
To provide an alternative for tests on live Varroa mites, we designed an assay that uses brush border membrane vesicles.
-
Varroa isolates
We tried to find our own Varroa entomopathogen.
-
Riboswitches
To detect the presence of Varroa mites in the beehive, we made a B12 and a guanine riboswitch.
-
Population Dynamics
A quorum sensing system was characterized to regulate toxin production in BeeT.
-
Optogenetic Kill Switch
The light-induced kill switch was one of our biocontainment methods.
-
Chitinases
As a back-up, we wanted to use chitinases which are more harmful to Varroa destructor than honeybees.
-
Cas9-based Kill Switch
The Cas9-based kill switch was one of our biocontainment methods.
-
Toxin Scanner
We made a software tool for finding new Cry proteins toxic to Varroa destructor.
-
E. coli survival
Following the results of the Metabolic Model, we needed more data.
-
Toggle Switch
We designed a toggle switch that could coordinate mites sensing, BT's toxin production and light sensing with fast responsiveness.
-
Human Outreach
We implemented our outreach into our research as much as we could. Find the details here!
-
BEEHAVE
We adapted the BEEHAVE model to include BeeT.
-
Population Model
Modelling of the population dynamics regulation system..
-
Kill Switch Model
The optogenetic kill switch was modeled with ODEs to understand the system in more detail.
-
Metabolic Modelling
We wanted to use a metabolic model to see the effect of our BeeT distribution method.
