Difference between revisions of "Team:Valencia UPV/HP/Silver"

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<html><head><meta charset="utf-8"/><meta name="keywords" content="HTML5,CSS3,Template"/> <meta name="viewport" content="width=device-width, maximum-scale=1, initial-scale=1, user-scalable=0"/> <meta name="description" content=""/> <title>HYPE IT - Valencia UPV</title><meta name="Author" content="Dorin Grigoras [www.stepofweb.com]"/></head><body class="smoothscroll enable-animation"><div id="wrapper"><div id="header" class="sticky clearfix"><header id="topNav"><div class="container"><button class="btn btn-mobile" data-toggle="collapse" data-target=".nav-main-collapse"><i class="fa fa-bars"></i></button><a class="logo pull-left" href="/"><img src="https://static.igem.org/mediawiki/2016/7/77/ValenciaUPV_HYPElogo.png" alt=""/></a><div class="navbar-collapse pull-right nav-main-collapse collapse submenu-dark"><nav class="nav-main"><ul id="topMain" class="nav nav-pills nav-main"> <li> <a href="https://2016.igem.org/Team:Valencia_UPV"> HOME </a> </li><li class="dropdown"><a class="dropdown-toggle" href="#">TEAM</a><ul class="dropdown-menu pull-right"><li><a href="https://2016.igem.org/Team:Valencia_UPV/Team">OUR TEAM</a></li><li><a href="https://2016.igem.org/Team:Valencia_UPV/Collaborations">COLLABORATIONS</a></li><li><a href="https://2016.igem.org/Team:Valencia_UPV/Attributions">ATTRIBUTIONS</a></li></ul></li><li class="dropdown"> <a class="dropdown-toggle" href="#"> PROJECT </a> <ul class="dropdown-menu pull-right"> <li><a href="https://2016.igem.org/Team:Valencia_UPV/Project">OUR PROJECT</a></li><li><a href="https://2016.igem.org/Team:Valencia_UPV/Design">DESIGN</a></li><li><a href="https://2016.igem.org/Team:Valencia_UPV/Software">DATA PROCESSING SOFTWARE</a></li><li><a href="https://2016.igem.org/Team:Valencia_UPV/Grna_testing_system">GRNA TESTING SYSTEM</a></li><li><a href="https://2016.igem.org/Team:Valencia_UPV/Split">SPLIT CAS9</a></li><li><a href="https://2016.igem.org/Team:Valencia_UPV/Hardware">LAB CASE</a></li><li><a href="https://2016.igem.org/Team:Valencia_UPV/Demonstrate">DEMONSTRATE</a></li><li><a href="https://2016.igem.org/Team:Valencia_UPV/Results">RESULTS</a></li><li><a href="https://2016.igem.org/Team:Valencia_UPV/notebook">NOTEBOOK</a></li></ul> </li><li class="dropdown"> <a class="dropdown-toggle" href="#"> HUMAN PRACTICES </a> <ul class="dropdown-menu pull-right"> <li><a href="https://2016.igem.org/Team:Valencia_UPV/Human_Practices">OUR HUMAN PRACTICES</a></li><li><a href="https://2016.igem.org/Team:Valencia_UPV/HP/Silver">SOCIAL ENGAGEMENT</a></li><li><a href="https://2016.igem.org/Team:Valencia_UPV/HP/Gold">GOLD</a></li><li><a href="https://2016.igem.org/Team:Valencia_UPV/Integrated_Practices">INTEGRATED PRACTICES</a></li><li><a href="https://2016.igem.org/Team:Valencia_UPV/Safety">SAFETY</a></li></ul> </li><li class="dropdown"> <a class="dropdown-toggle" href="#"> PARTS </a> <ul class="dropdown-menu pull-right"> <li><a href="https://2016.igem.org/Team:Valencia_UPV/Parts">Our PARTS</a></li><li><a href="https://2016.igem.org/Team:Valencia_UPV/Basic_Part">BASIC PARTS</a></li><li><a href="https://2016.igem.org/Team:Valencia_UPV/Composite_Part">COMPOSITEPARTS</a></li><li><a href="https://2016.igem.org/Team:Valencia_UPV/Part_Collection">PART COLLECTION</a></li></ul> </li><li> <a class="active" href="https://2016.igem.org/Team:Valencia_UPV/Model"> MODEL </a> </li></ul></nav></div></div></header></div><section class="page-header page-header-xs"><div class="container"><h1>MODEL</h1><span class="font-lato size-18 hidden-xs">We believe in Simple &amp; Creative</span></div></section><footer id="footer"> <div class="container"> <div class="row"> <div class="col-md-8"> <img class="footer-logo footer-2" width="200px" src="http://goldenbraid.cloudno.de/template/assets/images/logo_dark.png" alt=""> <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Maecenas metus nulla, commodo a sodales sed, dignissim pretium nunc. Nam et lacus neque.</p><hr> <div class="row"> <div class="col-md-6 col-sm-6 hidden-xs"> <div class="margin-left-50 clearfix"> <p class="margin-bottom-10">Follow Us</p><a href="#" class="social-icon social-icon-sm social-icon-transparent social-facebook pull-left" data-toggle="tooltip" data-placement="top" title="" data-original-title="Facebook"> <i class="icon-facebook"></i> <i class="icon-facebook"></i> </a> <a href="#" class="social-icon social-icon-sm social-icon-transparent social-twitter pull-left" data-toggle="tooltip" data-placement="top" title="" data-original-title="Twitter"> <i class="icon-twitter"></i> <i class="icon-twitter"></i> </a> <a href="#" class="social-icon social-icon-sm social-icon-transparent social-gplus pull-left" data-toggle="tooltip" data-placement="top" title="" data-original-title="Google plus"> <i class="icon-gplus"></i> <i class="icon-gplus"></i> </a> </div></div></div></div><div class="col-md-4"> <h4 class="letter-spacing-1">PHOTO GALLERY</h4> <div class="footer-gallery lightbox" data-plugin-options="{&quot;delegate&quot;: &quot;a&quot;, &quot;gallery&quot;:{&quot;enabled&quot;: true}}"> <a href="assets/images/demo/1200x800/10-min.jpg"> <img src="assets/images/demo/150x99/10-min.jpg" width="106" height="70" alt=""> </a> <a href="assets/images/demo/1200x800/19-min.jpg"> <img src="assets/images/demo/150x99/19-min.jpg" width="106" height="70" alt=""> </a> <a href="assets/images/demo/1200x800/12-min.jpg"> <img src="assets/images/demo/150x99/12-min.jpg" width="106" height="70" alt=""> </a> <a href="assets/images/demo/1200x800/13-min.jpg"> <img src="assets/images/demo/150x99/13-min.jpg" width="106" height="70" alt=""> </a> <a href="assets/images/demo/1200x800/18-min.jpg"> <img src="assets/images/demo/150x99/18-min.jpg" width="106" height="70" alt=""> </a> <a href="assets/images/demo/1200x800/15-min.jpg"> <img src="assets/images/demo/150x99/15-min.jpg" width="106" height="70" alt=""> </a> </div></div></div></div><div class="copyright"> <div class="container"> <ul class="pull-right nomargin list-inline mobile-block"> <li><a href="#">Terms &amp; Conditions</a></li><li>•</li><li><a href="#">Privacy</a></li></ul> © All Rights Reserved, Company LTD </div></div></footer></div></body></html>
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{{:Team:Valencia_UPV/Templates:generaljs}}
 
{{:Team:Valencia_UPV/Templates:generaljs}}
{{:Team:Valencia_UPV/Templates:headerUPV}}
 
<html>
 
<head>
 
  <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/css/bootstrap.min.css" integrity="sha384-BVYiiSIFeK1dGmJRAkycuHAHRg32OmUcww7on3RYdg4Va+PmSTsz/K68vbdEjh4u" crossorigin="anonymous">
 
  <script src="https://use.fontawesome.com/9f4f2432c5.js"></script>
 
  <style>
 
    header{
 
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      font-family: 'Lato',Arial,Helvetica,sans-serif !important;
 
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</head>
 
<div id="mainContent">
 
  <div class="row">
 
    <div class="col-xs-12">
 
      <h1>May</h1>
 
    </div>
 
    <div class="col-md-6">
 
      <div class="blog-post-item">
 
        <h2>May 18, 2016</h2>
 
        <p class="post">Take glycerinated cultures of C58 <i>Agrobacterium</i> with dsRED from GoldenBraid Collection. Prepare broth culture (5 mL LB + 5 μL kanamycin + 5 μL Rifampin) 1:1000 and incubate overnight at 28°C.</p>
 
      </div>
 
    </div>
 
    <div class="col-md-6">
 
      <div class="blog-post-item">
 
        <h2>May 19, 2016</h2>
 
        <p class="post">Refresh previously made culture by inoculating 10 μL in a new culture medium.</p>
 
      </div>
 
    </div>
 
  </div>
 
  <div class="row">
 
    <div class="col-md-6">
 
      <div class="blog-post-item">
 
        <h2>May 20, 2016</h2>
 
        <p class="post">Agroinfiltration in <i>Nicotiana benthamiana</i> of C58 with dsRED.
 
          <a href="" target="blank"></a>
 
        </p>
 
      </div>
 
    </div>
 
  </div>
 
  <div class="row">
 
    <div class="col-xs-12">
 
      <h1>June</h1>
 
    </div>
 
    <div class="col-md-6">
 
      <div class="blog-post-item">
 
        <h2>June 6, 2016</h2>
 
        <div class="post">
 
          <ul>
 
            <li>
 
              <p>Take from the glycerinates of Goldenbraid Collection: </p>
 
              <div class="table-responsive">
 
                <table class="table table-striped">
 
                  <thead>
 
                    <tr>
 
                      <th>Plasmid</th>
 
                      <th>GB Code</th>
 
                    </tr>
 
                  </thead>
 
                  <tbody>
 
                    <tr>
 
                      <td>pD6B3 α1</td>
 
                      <td>GB0015</td>
 
                    </tr>
 
                    <tr>
 
                      <td>pD6B3 α2</td>
 
                      <td>GB0017</td>
 
                    </tr>
 
                    <tr>
 
                      <td>pD6B3 Ω1</td>
 
                      <td>GB0019</td>
 
                    </tr>
 
                    <tr>
 
                      <td>pD6B3 Ω2</td>
 
                      <td>GB0021</td>
 
                    </tr>
 
                    <tr>
 
                      <td>pUPD2</td>
 
                      <td>GB0307</td>
 
                    </tr>
 
                  </tbody>
 
                </table>
 
              </div>
 
            </li>
 
            <li>
 
              <p>Prepare liquid culture (3 mL LB + 3 μL antibiotic) 1:1000. Incubate at 37°C overnight.</p>
 
            </li>
 
            <li>Experiment with snails:
 
              <ul>
 
                <li>
 
                  <p>Let both <i>N. benthamiana</i>leafs with snails overnight at room temperature in separated boxes. We will observe if snails eat the leafs and if appears fluorescence.</p>
 
                </li>
 
              </ul>
 
            </li>
 
          </ul>
 
        </div>
 
      </div>
 
    </div>
 
  </div>
 
</div>
 
</html>
 

Revision as of 22:30, 6 September 2016

HYPE IT - Valencia UPV

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