Difference between revisions of "GDSYZX-United/NOTEBOOK/day note"
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nav {padding-top:10px;} | nav {padding-top:10px;} | ||
| − | + | .text_font { | |
| − | line-height: | + | line-height:120%; |
| − | font-size: | + | font-size:130% |
| − | + | } | |
</style> | </style> | ||
</head> | </head> | ||
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<div class="row"> | <div class="row"> | ||
<div class="col-xs-12 text-center"> | <div class="col-xs-12 text-center"> | ||
| − | < | + | <h1>Day note</h1> |
</div> | </div> | ||
</div> | </div> | ||
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<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i>< | + | <i class="fa fa-file-text"></i><div class="text_font"> 2016.7.31</div> |
| − | <small class="text-navy">< | + | <small class="text-navy"><div class="text_font">9:00</div></small> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<h3 class="m-b-xs">Extract genome from Arabidopsis Thaliana</h3> | <h3 class="m-b-xs">Extract genome from Arabidopsis Thaliana</h3> | ||
| − | < | + | <div class="text_font">The genome has been degraded greatly,possibly because we didn’t grind the leaves in very low temperature to inhibit the nuclease.We have to do it again.</div> |
</div> | </div> | ||
</div> | </div> | ||
| Line 124: | Line 124: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i>< | + | <i class="fa fa-file-text"></i><div class="text_font"> 2016.7.31</div> |
| − | <small class="text-navy">< | + | <small class="text-navy"><div class="text_font">13:30</div></small> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<p class="m-b-xs"><strong>Extract genome from Arabidopsis Thaliana</strong> | <p class="m-b-xs"><strong>Extract genome from Arabidopsis Thaliana</strong> | ||
| − | </ | + | </div> |
| − | < | + | <div class="text_font"> |
We extracted it the genome successfully.But the genome has still been degraded a little. (Since we don’t have liquid nitrogen in our lab,so we grinded the leaves in an ice box. )We will use the genome to do the following PCR. | We extracted it the genome successfully.But the genome has still been degraded a little. (Since we don’t have liquid nitrogen in our lab,so we grinded the leaves in an ice box. )We will use the genome to do the following PCR. | ||
| − | </ | + | </div> |
</div> | </div> | ||
</div> | </div> | ||
| Line 139: | Line 139: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i>< | + | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.1</div> |
| − | <small class="text-navy">< | + | <small class="text-navy"><div class="text_font">9:00</div></small> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<p class="m-b-xs"><strong>We use PCR to generate and to amplify our target DNA fragments:promotercop1,promoter phyB,promoterpif1,gene hhl1,gene pHhl,gene flu.</strong> | <p class="m-b-xs"><strong>We use PCR to generate and to amplify our target DNA fragments:promotercop1,promoter phyB,promoterpif1,gene hhl1,gene pHhl,gene flu.</strong> | ||
| − | </ | + | </div> |
| − | < | + | <div class="text_font"> |
We failed to get all our target DNA fragments .We will check the primers and the PCR program ,and then do it again. | We failed to get all our target DNA fragments .We will check the primers and the PCR program ,and then do it again. | ||
| − | </ | + | </div> |
</div> | </div> | ||
</div> | </div> | ||
| Line 154: | Line 154: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i>< | + | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.2</div> |
| − | <small class="text-navy">< | + | <small class="text-navy"><div class="text_font">9:00</div></small> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<p class="m-b-xs"><strong>Do the PCR again to get our target DNA fragments.</strong> | <p class="m-b-xs"><strong>Do the PCR again to get our target DNA fragments.</strong> | ||
| − | </ | + | </div> |
| − | < | + | <div class="text_font"> |
We PCR gene pHhl1,gene hhl1,gene flu successfully. | We PCR gene pHhl1,gene hhl1,gene flu successfully. | ||
We will PCR cop1 together with pif1,phyB tomorrow. | We will PCR cop1 together with pif1,phyB tomorrow. | ||
| − | </ | + | </div> |
</div> | </div> | ||
</div> | </div> | ||
| Line 170: | Line 170: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i>< | + | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.3</div> |
| − | <small class="text-navy">< | + | <small class="text-navy"><div class="text_font">9:00</div></small> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<p class="m-b-xs"><strong>We use PCR to get cop1,phyB and pif1</strong> | <p class="m-b-xs"><strong>We use PCR to get cop1,phyB and pif1</strong> | ||
| − | </ | + | </div> |
| − | < | + | <div class="text_font"> |
In the first PCR we get cop 1 and pif1,we get higher concentration of phyB in the second time PCR. | In the first PCR we get cop 1 and pif1,we get higher concentration of phyB in the second time PCR. | ||
| − | </ | + | </div> |
</div> | </div> | ||
</div> | </div> | ||
| Line 185: | Line 185: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i>< | + | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.3</div> |
| − | <small class="text-navy">< | + | <small class="text-navy"><div class="text_font">13:30</div></small> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<p class="m-b-xs"><strong>We use a kit box to purify our PCR products,so we can get our genes.</strong> | <p class="m-b-xs"><strong>We use a kit box to purify our PCR products,so we can get our genes.</strong> | ||
| − | </ | + | </div> |
| − | < | + | <div class="text_font"> |
We did it. | We did it. | ||
| − | </ | + | </div> |
</div> | </div> | ||
</div> | </div> | ||
| Line 200: | Line 200: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i>< | + | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.3</div> |
| − | <small class="text-navy">< | + | <small class="text-navy"><div class="text_font">in the afternoon</div></small> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<p class="m-b-xs"><strong>We cut our DNA fragments using restriction enzymes:BsaⅠ,PstⅠ, | <p class="m-b-xs"><strong>We cut our DNA fragments using restriction enzymes:BsaⅠ,PstⅠ, | ||
EcorⅠ.After this process ,the gene fragments will have one overhang at both ends.The overhangs will help them splice other fragments. </strong> | EcorⅠ.After this process ,the gene fragments will have one overhang at both ends.The overhangs will help them splice other fragments. </strong> | ||
| − | </ | + | </div> |
| − | < | + | <div class="text_font"> |
We did it. | We did it. | ||
| − | </ | + | </div> |
</div> | </div> | ||
</div> | </div> | ||
| Line 216: | Line 216: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i>< | + | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.4</div> |
| − | <small class="text-navy">< | + | <small class="text-navy"><div class="text_font">9:00</div></small> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<p class="m-b-xs"><strong>We purify our genes from the restriction enzyme systems.</strong> | <p class="m-b-xs"><strong>We purify our genes from the restriction enzyme systems.</strong> | ||
| − | </ | + | </div> |
| − | < | + | <div class="text_font"> |
We did it. | We did it. | ||
| − | </ | + | </div> |
</div> | </div> | ||
</div> | </div> | ||
| Line 231: | Line 231: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i>< | + | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.4</div> |
| − | <small class="text-navy">< | + | <small class="text-navy"><div class="text_font">in the morning</div></small> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<p class="m-b-xs"><strong>We cut our plasmids psb3c1 with BsaⅠ.</strong> | <p class="m-b-xs"><strong>We cut our plasmids psb3c1 with BsaⅠ.</strong> | ||
| − | </ | + | </div> |
| − | < | + | <div class="text_font"> |
We did it. | We did it. | ||
| − | </ | + | </div> |
</div> | </div> | ||
</div> | </div> | ||
| Line 247: | Line 247: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i>< | + | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.4</div> |
| − | <small class="text-navy">< | + | <small class="text-navy"><div class="text_font">in the morning</div></small> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<p class="m-b-xs"><strong>We splice our DNA fragments to get the parts we want using | <p class="m-b-xs"><strong>We splice our DNA fragments to get the parts we want using | ||
a kit box.</strong> | a kit box.</strong> | ||
| − | </ | + | </div> |
| − | < | + | <div class="text_font"> |
We did it. | We did it. | ||
| − | </ | + | </div> |
</div> | </div> | ||
</div> | </div> | ||
| Line 263: | Line 263: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i>< | + | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.4</div> |
| − | <small class="text-navy">< | + | <small class="text-navy"><div class="text_font">13:30</div></small> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<p class="m-b-xs"><strong>We purify our parts using a kit box.</strong> | <p class="m-b-xs"><strong>We purify our parts using a kit box.</strong> | ||
| − | </ | + | </div> |
| − | < | + | <div class="text_font"> |
We did it. | We did it. | ||
| − | </ | + | </div> |
</div> | </div> | ||
</div> | </div> | ||
| Line 278: | Line 278: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i>< | + | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.4</div> |
| − | <small class="text-navy">< | + | <small class="text-navy"><div class="text_font">in the afternoon</div></small> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<p class="m-b-xs"><strong>We cut our parts with BsaⅠto | <p class="m-b-xs"><strong>We cut our parts with BsaⅠto | ||
make sure there will be no loops in the part.</strong> | make sure there will be no loops in the part.</strong> | ||
| − | </ | + | </div> |
| − | < | + | <div class="text_font"> |
We did it. | We did it. | ||
| − | </ | + | </div> |
</div> | </div> | ||
</div> | </div> | ||
| Line 294: | Line 294: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i>< | + | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.4</div> |
| − | <small class="text-navy">< | + | <small class="text-navy"><div class="text_font">in the afternoon</div></small> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<p class="m-b-xs"><strong>We purify the parts.</strong> | <p class="m-b-xs"><strong>We purify the parts.</strong> | ||
| − | </ | + | </div> |
| − | < | + | <div class="text_font"> |
We did it. | We did it. | ||
| − | </ | + | </div> |
</div> | </div> | ||
</div> | </div> | ||
| Line 309: | Line 309: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i>< | + | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.4</div> |
| − | <small class="text-navy">< | + | <small class="text-navy"><div class="text_font">in the afternoon</div></small> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<p class="m-b-xs"><strong>We splice our parts with plasmids.</strong> | <p class="m-b-xs"><strong>We splice our parts with plasmids.</strong> | ||
| − | </ | + | </div> |
| − | < | + | <div class="text_font"> |
We did it. | We did it. | ||
| − | </ | + | </div> |
</div> | </div> | ||
</div> | </div> | ||
| Line 324: | Line 324: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i>< | + | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.4</div> |
| − | <small class="text-navy">< | + | <small class="text-navy"><div class="text_font">in the afternoon</div></small> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<p class="m-b-xs"><strong>We prepare LB broth.</strong> | <p class="m-b-xs"><strong>We prepare LB broth.</strong> | ||
| − | </ | + | </div> |
| − | < | + | <div class="text_font"> |
We did it. | We did it. | ||
| − | </ | + | </div> |
</div> | </div> | ||
</div> | </div> | ||
| Line 339: | Line 339: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i>< | + | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.4</div> |
| − | <small class="text-navy">< | + | <small class="text-navy"><div class="text_font">19:00</div></small> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<p class="m-b-xs"><strong>We transform our plasmids into DH5α.</strong> | <p class="m-b-xs"><strong>We transform our plasmids into DH5α.</strong> | ||
| − | </ | + | </div> |
| − | < | + | <div class="text_font"> |
We did it. | We did it. | ||
| − | </ | + | </div> |
</div> | </div> | ||
</div> | </div> | ||
| Line 354: | Line 354: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i>< | + | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.4</div> |
| − | <small class="text-navy">< | + | <small class="text-navy"><div class="text_font">in the evening</div></small> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<p class="m-b-xs"><strong>We inoculate Ecoli DH5α on LB plates and in media.</strong> | <p class="m-b-xs"><strong>We inoculate Ecoli DH5α on LB plates and in media.</strong> | ||
| − | </ | + | </div> |
| − | < | + | <div class="text_font"> |
We did it. | We did it. | ||
| − | </ | + | </div> |
</div> | </div> | ||
</div> | </div> | ||
| Line 369: | Line 369: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i>< | + | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.5</div> |
| − | <small class="text-navy">< | + | <small class="text-navy"><div class="text_font">13:00</div></small> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<p class="m-b-xs"><strong>We check the growth of Ecoli on LB plates.</strong> | <p class="m-b-xs"><strong>We check the growth of Ecoli on LB plates.</strong> | ||
| − | </ | + | </div> |
| − | < | + | <div class="text_font"> |
No conlony was observed. | No conlony was observed. | ||
So we will use the Ecoli in media to do the following experiments. | So we will use the Ecoli in media to do the following experiments. | ||
| − | </ | + | </div> |
</div> | </div> | ||
</div> | </div> | ||
| Line 386: | Line 386: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i>< | + | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.5</div> |
| − | <small class="text-navy">< | + | <small class="text-navy"><div class="text_font">in the afternoon</div></small> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<p class="m-b-xs"><strong>We extract plasmids from the solution.</strong> | <p class="m-b-xs"><strong>We extract plasmids from the solution.</strong> | ||
| − | </ | + | </div> |
| − | < | + | <div class="text_font"> |
We did it. | We did it. | ||
| − | </ | + | </div> |
</div> | </div> | ||
</div> | </div> | ||
| Line 401: | Line 401: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i>< | + | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.6</div> |
| − | <small class="text-navy">< | + | <small class="text-navy"><div class="text_font">9:00</div></small> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<p class="m-b-xs"><strong>We prepare Arabidopsis protoplasts.</strong> | <p class="m-b-xs"><strong>We prepare Arabidopsis protoplasts.</strong> | ||
| − | </ | + | </div> |
| − | < | + | <div class="text_font"> |
The concentration of protoplasts is too low,this is because we chose the wrong filter paper. | The concentration of protoplasts is too low,this is because we chose the wrong filter paper. | ||
| − | </ | + | </div> |
</div> | </div> | ||
</div> | </div> | ||
| Line 416: | Line 416: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i>< | + | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.6</div> |
| − | <small class="text-navy">< | + | <small class="text-navy"><div class="text_font">13:00</div></small> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<p class="m-b-xs"><strong>We prepare protoplasts again.</strong> | <p class="m-b-xs"><strong>We prepare protoplasts again.</strong> | ||
| − | </ | + | </div> |
| − | < | + | <div class="text_font"> |
We did it.The concentration is much higher,and there is much more sediment of protoplast after centrifuging. | We did it.The concentration is much higher,and there is much more sediment of protoplast after centrifuging. | ||
| − | </ | + | </div> |
</div> | </div> | ||
</div> | </div> | ||
| Line 431: | Line 431: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i>< | + | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.6</div> |
| − | <small class="text-navy">< | + | <small class="text-navy"><div class="text_font">in the afternoon</div></small> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| Line 438: | Line 438: | ||
the protoplasts in the following light intensity (lux):0,3960,7920,11880. | the protoplasts in the following light intensity (lux):0,3960,7920,11880. | ||
</strong> | </strong> | ||
| − | </ | + | </div> |
| − | < | + | <div class="text_font"> |
We did it. | We did it. | ||
| − | </ | + | </div> |
</div> | </div> | ||
</div> | </div> | ||
| Line 448: | Line 448: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i>< | + | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.7</div> |
| − | <small class="text-navy">< | + | <small class="text-navy"><div class="text_font">9:00</div></small> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<p class="m-b-xs"><strong>We extract RNA from protoplasts using a kit box.</strong> | <p class="m-b-xs"><strong>We extract RNA from protoplasts using a kit box.</strong> | ||
| − | </ | + | </div> |
| − | < | + | <div class="text_font"> |
We did it. | We did it. | ||
| − | </ | + | </div> |
</div> | </div> | ||
</div> | </div> | ||
| Line 463: | Line 463: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i>< | + | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.7</div> |
| − | <small class="text-navy">< | + | <small class="text-navy"><div class="text_font">in the morning</div></small> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| Line 470: | Line 470: | ||
to amplify hhl1 to see how much hhl1 is transcripted into mRNA. | to amplify hhl1 to see how much hhl1 is transcripted into mRNA. | ||
</strong> | </strong> | ||
| − | </ | + | </div> |
| − | < | + | <div class="text_font"> |
We failed to get any hhl1. There may be something wrong with the process of transformation. | We failed to get any hhl1. There may be something wrong with the process of transformation. | ||
| − | </ | + | </div> |
</div> | </div> | ||
</div> | </div> | ||
| Line 480: | Line 480: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i>< | + | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.10</div> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<p class="m-b-xs"><strong>PCR our target DNA fragments:cop1,pif1,phyB,hhl1,pHhl1-hhl1,flu.</strong> | <p class="m-b-xs"><strong>PCR our target DNA fragments:cop1,pif1,phyB,hhl1,pHhl1-hhl1,flu.</strong> | ||
| − | </ | + | </div> |
| − | < | + | <div class="text_font"> |
We will check the result tomorrow. | We will check the result tomorrow. | ||
| − | </ | + | </div> |
</div> | </div> | ||
</div> | </div> | ||
Revision as of 14:54, 29 September 2016
Day note
2016.7.31
9:00
Extract genome from Arabidopsis Thaliana
The genome has been degraded greatly,possibly because we didn’t grind the leaves in very low temperature to inhibit the nuclease.We have to do it again.
2016.7.31
13:30
Extract genome from Arabidopsis Thaliana
We extracted it the genome successfully.But the genome has still been degraded a little. (Since we don’t have liquid nitrogen in our lab,so we grinded the leaves in an ice box. )We will use the genome to do the following PCR.
2016.8.1
9:00
We use PCR to generate and to amplify our target DNA fragments:promotercop1,promoter phyB,promoterpif1,gene hhl1,gene pHhl,gene flu.
We failed to get all our target DNA fragments .We will check the primers and the PCR program ,and then do it again.
2016.8.2
9:00
Do the PCR again to get our target DNA fragments.
We PCR gene pHhl1,gene hhl1,gene flu successfully.
We will PCR cop1 together with pif1,phyB tomorrow.
2016.8.3
9:00
We use PCR to get cop1,phyB and pif1
In the first PCR we get cop 1 and pif1,we get higher concentration of phyB in the second time PCR.
2016.8.3
13:30
We use a kit box to purify our PCR products,so we can get our genes.
We did it.
2016.8.3
in the afternoon
We cut our DNA fragments using restriction enzymes:BsaⅠ,PstⅠ, EcorⅠ.After this process ,the gene fragments will have one overhang at both ends.The overhangs will help them splice other fragments.
We did it.
2016.8.4
9:00
We purify our genes from the restriction enzyme systems.
We did it.
2016.8.4
in the morning
We cut our plasmids psb3c1 with BsaⅠ.
We did it.
2016.8.4
in the morning
We splice our DNA fragments to get the parts we want using a kit box.
We did it.
