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</div> | </div> | ||
− | < | + | <div>We tried forming multimers using the self-assembling peptide, P11-4 and RADA-16. We |
Connected short linker(GGCGG) and SAP to both ends of the protein. In this experiment, we | Connected short linker(GGCGG) and SAP to both ends of the protein. In this experiment, we | ||
formed the multimers of GFP. GFP’s molecular mass is 26891Da. When fusing with P<sub>11</sub>-4, it’s | formed the multimers of GFP. GFP’s molecular mass is 26891Da. When fusing with P<sub>11</sub>-4, it’s | ||
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to separate the proteins with less than 50KDa. We irradiated 480nm light to filtrate and observed | to separate the proteins with less than 50KDa. We irradiated 480nm light to filtrate and observed | ||
whether 580nm wave-length light was emitted. | whether 580nm wave-length light was emitted. | ||
− | </ | + | </div> |
</div> | </div> | ||
Revision as of 03:35, 1 October 2016
Team:HokkaidoU Japan
We advocate the new method to create multimer using the self-assembling ability of SAP. As fusing SAP to the end of protein, it will condense with other proteins’ SAP domains and form the complex. BY using this method, we’ll be able to connect several enzymes and allow huge complexed proteins formed. It’ll improve the efficiency of continuous reaction. The ordinary method uses linkers to connect proteins. We suggest this new method with SAP is superior to the ordinary one for these resons.
Linker Method | SAP Method |
---|---|
It is difficult to connect several huge proteins | It is possible to synthesize the proteins individually. It also can form huge complex |
Regulated by one promoter | Each proteins can be expressed individually |
The possibility of deformation of 3D-structure | Low possibility of deformation since they connect with only proteins which can condense |
There are also disadvantages using SAP. Since the number of the possible combination of several different is infinite, there is no guarantee that we can always obtain the expected combination when we form the protein complex. The one solution for the problem is limiting the number of combination by using different SAP. As forming protein complex with different functions, this multimer forming method with SAP let us create more functional unit. When same kind of proteins are used, it’ll be large block and its function is expected to be enhanced.
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