Difference between revisions of "Team:Wageningen UR/Notebook/ProteinEngineering"

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<h1><b>August</b></h1>
 
<h1><b>August</b></h1>
 
<h2><b>Week 13</b></h2>
 
<h2><b>Week 13</b></h2>
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<h2><b>Week 18-19</b></h2>
 
<h2><b>Week 18-19</b></h2>

Revision as of 15:07, 11 October 2016

Wageningen UR iGEM 2016

 

May

Week 1

Setting up the lab, preparing media, preparing electrocompetent cells, primer design etc.

Week 2

PCR of Cry3Aa from Bacillus thuringiensis var. tenebrionis and Cry1Ab from Bacillus thuringiensis Berliner 1915. Success with PCR of Cry3Aa, but not Cry1Ab. Creation of vector pBbA7c-Cry3Aa.

Week 3

I tried to transform the created vector pBbA7c-Cry3Aa into Lemo21. No success.

Week 4

A lot of time spent on trying to clone the vector pBbA7c/Cry3Aa into Lemo21. Inconclusive results.

June

Week 5-8

No chance of working in the lab due to moving of the lab.

July

Week 9

A fresh start with a lot of work for setting up the lab again. I learned the basics of phage work in this and the next week.

Week 10

Continuation of learning how to work with bacteriophages. Mite feeding experiments with fluorophores: Mites do take up fluorophores with their food! But only a small amount of them (~16 %).

Week 11

Feeding experiment with mealworms. Mealworms are easy to feed, easy to dissect and easy to keep. They take up fluorescine when fed with carrots dipped in it.

Week 12

vacation

August

Week 13

Week 18-19

Week 20

September

Week 21

Week 22