Difference between revisions of "Team:Lethbridge/Safety"

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{{Lethbridge}}
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<head>
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{{Lethbridge/MaterializeJS}}
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  <!-- Wiki Style Reset (From U of C)-->
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{{Lethbridge/InitJS}}
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    <style type="text/css">
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{{Lethbridge/Navbar}}
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      /* Reset the wiki style by overriding with a reset stylesheet.
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        The relevant wiki css rules are reset by using rules with higher priority.
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        (Written by Florian Albrecht)
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        Shoutout to the 2016 U of C wiki */
  
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<html>
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      /* Start general reset ================================================== */
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        html body {font-size: inherit;font-family: inherit;background-color: transparent;color: inherit;padding: 0;}
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        div.right-menu li a {background-color: transparent;color: inherit;padding: 0;font-family: 'Roboto', sans-serif;}
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        div.right-menu li a:hover {color: inherit;text-decoration: none;font-family: 'Roboto', sans-serif;}
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        div#search-controls {background-color: transparent;height: auto;position: static;right: 0;text-align: left;top: 0;width: auto;z-index: auto;}
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      /* Start content reset ================================================== */
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        div#content {background: transparent;border: 0;color: inherit;line-height: inherit;margin: 0;padding: 0;position: static;width: auto;z-index: auto;}
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        a:link, a:hover, a:active, a:visited{color: white; text-decoration: none;font-family: 'Roboto', sans-serif;}
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        body li {margin: 0; font-family: 'Roboto', sans-serif;}
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        body h1, body h2, body h3, body h4, body h5, body h6 {background: transparent;border: 0;color: inherit;padding: 0; font-family: 'Roboto', sans-serif;}
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        body .firstHeading {margin: 0;}
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        div#contentSub {color: inherit;font-size: inherit;line-height: inherit;margin: 0;width: auto;}
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        div#catlinks {background-color: transparent;border: 0;clear: both;margin: 0;padding: 0;}
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      /* Start footer reset =================================================== */
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        div#footer-box {background-color: transparent;border: 0;margin: 0;padding: 0;width: auto;}
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        div#footer {color: inherit;font-size: inherit;text-align: left;}
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        div#footer li {margin: 0;display: list-item; font-family: 'Roboto', sans-serif;}
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      </style>
  
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      <!-- SideMenu for iGEM Stuff -->
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      <style type="text/css">
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      #sideMenu{width: 200px;position: absolute;top: 20px;left: 1020px;z-index: 10;padding-top: 0px;padding-bottom: 15px;padding-left: 15px;padding-right: 15px;background-color: white;text-align: left;display: none;z-index: 9996;}
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      #top_menu_under{height: 0px;}
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      #globalWrapper{padding-bottom: 200px;}
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      </style>
  
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      <!-- Custom Styling -->
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      <style>
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      a {list-style-image: none;}
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      ul {list-style-image: none;}
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      ol {list-style-image: none;}
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      li {list-style-image: none;}
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      </style>
  
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<div class="column full_size">
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    <!-- Import Google Icon Font -->
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    <link href="http://fonts.googleapis.com/icon?family=Material+Icons" rel="stylesheet">
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    <!-- Roboto Font -->
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    <link href="https://fonts.googleapis.com/css?family=Roboto" rel="stylesheet">
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    <!--Import materialize.css-->
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    <link type="text/css" rel="stylesheet" href="https://2016.igem.org/Template:Lethbridge/MaterializeCSS?action=raw&ctype=text/css"  media="screen,projection"/>
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    <!--Let browser know website is optimized for mobile-->
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    <meta name="viewport" content="width=device-width, initial-scale=1.0"/>
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    <!-- Page Title -->
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    <title>Lethbridge iGEM 2016</title>
  
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</head>
  
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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<body>
  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<main>
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<div id="content">
  
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</div>
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  <nav class="red" role="navigation" style="margin-top: 15px;">
 +
    <div class="nav-wrapper">
 +
      <a href="https://2016.igem.org/Team:Lethbridge/Human_Practices" class="brand-logo left" style="width:auto; left:40px;"> Lethbridge iGEM</a>
 +
      <ul class="right hide-on-med-and-down" style="list-style-image: none;"><!--For the top bar-->
 +
        <li><a class="dropdown-button" href="#!" data-activates="dropdown1" style="display: block; padding-top: 19px; padding-bottom: 19px">Project</a></li>
 +
          <ul id='dropdown1' class='dropdown-content'>
 +
            <li><a href="https://2016.igem.org/Team:Lethbridge/Description" style="color:red;">Description</a></li>
 +
            <li><a href="https://2016.igem.org/Team:Lethbridge/Design" style="color:red;">Design</a></li>
 +
            <li><a href="https://2016.igem.org/Team:Lethbridge/Experiments" style="color:red;">Experiments</a></li>
 +
            <li><a href="https://2016.igem.org/Team:Lethbridge/Results" style="color:red;">Results</a></li>
 +
            <li><a href="https://2016.igem.org/Team:Lethbridge/Notebook" style="color:red;">Notebook</a></li>
 +
            <li><a href="https://2016.igem.org/Team:Lethbridge/Parts" style="color:red;">Parts</a></li>
 +
            <li><a href="https://2016.igem.org/Team:Lethbridge/Safety" style="color:red;">Safety</a></li>
 +
          </ul>
 +
        <li><a class="dropdown-button" href="#!" data-activates="dropdown2" style="display: block; padding-top: 19px; padding-bottom: 19px">Practices</a></li>
 +
          <ul id='dropdown2' class='dropdown-content' style="list-style-image: none;">
 +
            <li><a href="https://2016.igem.org/Team:Lethbridge/Human_Practices" style="color:red;">Introduction</a></li>
 +
            <li><a href="#!" style="color:red;">Literature Review</a></li><!--NEEDS STUFF-->
 +
            <li><a href="#!" style="color:red;">Interviews</a></li><!--NEEDS STUFF-->
 +
            <li><a href="https://2016.igem.org/Team:Lethbridge/Ridealong" style="color:red;">Ridealongs</a></li>
 +
            <li><a href="https://2016.igem.org/Team:Lethbridge/Integrated_Practices" style="color:red;">Integrated Practices</a></li>
 +
        </ul>
 +
        <li><a class="dropdown-button" href="#!" data-activates="dropdown3" style="display: block; padding-top: 19px; padding-bottom: 19px">Team</a></li>
 +
          <ul id='dropdown3' class="dropdown-content" style="list-style-image: none;">
 +
            <li><a href="https://2016.igem.org/Team:Lethbridge/Team" style="color:red;">Members</a></li>
 +
            <li><a href="https://2016.igem.org/Team:Lethbridge/Collaborations" style="color:red;">Collaborations</a></li>
 +
            <li><a href="https://2016.igem.org/Team:Lethbridge/Attributions" style="color:red;">Attributions</a></li>
 +
            <li><a href="https://2016.igem.org/Team:Lethbridge/Sponsors" style="color:red;">Sponsors</a></li>
 +
          </ul>
 +
        <li><a href="https://2016.igem.org/Team:Lethbridge/Achievements" style="display: block; padding-top: 19px; padding-bottom: 19px">Achievements</a></li>
 +
        <li><a href="https://2016.igem.org/Team:Lethbridge/Database" style="display: block; padding-top: 19px; padding-bottom: 19px">Database</a></li>
 +
      </ul>
  
 +
      <a href="#" data-activates="nav-mobile" class="button-collapse"><i class="material-icons">menu</i></a>
 +
        <ul id="nav-mobile" class="side-nav" style="margin-top:20px; margin-left:0px; margin-right:0px; margin-bottom:0px;">
 +
            <li class="no-padding">
 +
              <ul class="collapsible collapsible-accordion">
 +
                <li class="bold"><a class="collapsible-header waves-effect waves-teal">Project</a>
 +
                  <div class="collapsible-body">
 +
                    <ul>
 +
                      <li><a href="https://2016.igem.org/Team:Lethbridge/Description">Description</a></li>
 +
                      <li><a href="https://2016.igem.org/Team:Lethbridge/Design">Design</a></li>
 +
                      <li><a href="https://2016.igem.org/Team:Lethbridge/Experiments">Experiments</a></li>
 +
                      <li><a href="https://2016.igem.org/Team:Lethbridge/Results">Results</a></li>
 +
                      <li><a href="https://2016.igem.org/Team:Lethbridge/Notebook">Notebook</a></li>
 +
                      <li><a href="https://2016.igem.org/Team:Lethbridge/Parts">Parts</a></li>
 +
                      <li><a href="https://2016.igem.org/Team:Lethbridge/Safety">Safety</a></li>
 +
                    </ul>
 +
                  </div>
 +
                </li>
 +
              </ul>
 +
            </li>
  
−
<div class="column full_size">
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          <li class="no-padding">
−
<h5>Safe Project Design</h5>
+
            <ul class="collapsible collapsible-accordion">
 +
              <li class="bold"><a class="collapsible-header waves-effect waves-red">Practices</a>
 +
                <div class="collapsible-body">
 +
                  <ul>
 +
                    <li><a href="https://2016.igem.org/Team:Lethbridge/Human_Practices">Introduction</a></li>
 +
                    <li><a href="">Literature Review</a></li>
 +
                    <li><a href="">Interviews</a></li>
 +
                    <li><a href=https://2016.igem.org/Team:Lethbridge/Ridealong>Ridealongs</a></li>
 +
                    <li><a href="https://2016.igem.org/Team:Lethbridge/Integrated_Practices">Integrated Practices</a></li>
 +
                  </ul>
 +
                </div>
 +
              </li>
 +
            </ul>
 +
          </li>
  
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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        <li class="no-padding">
 +
          <ul class="collapsible collapsible-accordion">
 +
            <li class="bold"><a class="collapsible-header waves-effect waves-orange">Team</a>
 +
              <div class="collapsible-body">
 +
                <ul>
 +
                  <li><a href="https://2016.igem.org/Team:Lethbridge/Team">Members</a></li>
 +
                  <li><a href="https://2016.igem.org/Team:Lethbridge/Collaborations">Collaborations</a></li>
 +
                  <li><a href="https://2016.igem.org/Team:Lethbridge/Attributions">Attributions</a></li>
 +
                  <li><a href="https://2016.igem.org/Team:Lethbridge/Sponsors">Sponsors</a></li>
 +
                </ul>
 +
              </div>
 +
            </li>
 +
          </ul>
 +
        </li>
 +
        <li class="bold"><a class="collapsible-header waves-effect waves-orange" href="https://2016.igem.org/Team:Lethbridge/Achievements">Achievements</a></li>
 +
        <li class="bold"><a class="collapsible-header waves-effect waves-orange" href="https://2016.igem.org/Team:Lethbridge/Database">Database</a></li>
 +
    </ul>
 +
  </div>
 +
</nav>
  
−
<ul>
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  <div class="section no-pad-bot" id="index-banner">
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<li>Choosing a non-pathogenic chassis</li>
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    <div class="cardbg">
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<li>Choosing parts that will not harm humans / animals / plants</li>
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      <div class="container transparent">
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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        <br><br>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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        <h1 class="header center red-text">Nanoresponders</h1>
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</ul>
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        <div class="row center">
 +
          <h5 class="header col s12 light">Single-domain antibody production.</h5>
 +
        </div>
 +
        <div class="row center">
 +
          <a href="#" id="download-button" class="btn-large waves-effect waves-light red">Explore</a>
 +
        </div>
 +
        <br><br>
 +
      </div>
 +
    </div>
 +
  </div>
  
−
</div>
+
  <div class="col s12 red">
 +
    <div class="container">
 +
      <div class="row">
 +
        <div class="col l6 s12">
 +
          <h5 class="white-text">Introduction</h5>
 +
          <p class="grey-text text-lighten-4">The prevalence of virulent and multiple antibiotic resistant pathogens in healthcare facilities has resulted in ongoing reassessment of best practices to prevent their transmission. However, whether pathogen reservoirs exist in emergency medical services (EMS) vehicles, remains largely unknown. Our iGEM team developed a custom bacterial two hybrid system to select single-domain antibodies (nanobodies) that recognize human pathogens. Informed by our Nanopore next generation sequencing of DNA samples from ambulance vehicles, antibodies targeting important pathogens were then used to develop a rapid and low cost ELISA-based testing kit that may be employed on-site by EMS workers. Our project provides a framework for rapid detection of emergent pathogens and a practical and rapid solution for monitoring their presence in and outside of the healthcare system.</p>
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
  
−
<div class="column half_size">
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  <div class="row">
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<h5>Safe Lab Work</h5>
+
    <div class="row">
 +
      <div class="col s12 m6">
 +
        <div class="card blue-grey darken-1">
 +
          <div class="card-content white-text">
 +
            <span class="card-title">Card Title</span>
 +
            <p>I am a very simple card. I am good at containing small bits of information.
 +
            I am convenient because I require little markup to use effectively.</p>
 +
          </div>
 +
          <div class="card-action">
 +
            <a href="#">This is a link</a>
 +
            <a href="#">This is a link</a>
 +
          </div>
 +
        </div>
 +
      </div>
 +
    </div>
  
−
<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
+
    <div class="row">
 +
      <div class="col s12 m5 offset-m6">
 +
        <div class="card-panel teal z-depth-3">
 +
          <span class="white-text">I am a very simple card. I am good at containing small bits of information.
 +
          I am convenient because I require little markup to use effectively. I am similar to what is called a panel in other frameworks.
 +
          </span>
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
  
−
</div>
+
  </main>
  
−
<div class="column half_size">
+
  <footer class="page-footer blue">
−
<h5>Safe Shipment</h5>
+
    <div class="container">
 +
      <div class="row">
 +
        <div class="col l6 s12">
 +
          <h5 class="white-text">Overview</h5>
 +
          <p class="grey-text text-lighten-4">The prevalence of virulent and multiple antibiotic resistant pathogens in healthcare facilities has resulted in ongoing reassessment of best practices to prevent their transmission. However, whether pathogen reservoirs exist in emergency medical services (EMS) vehicles, remains largely unknown. Our iGEM team developed a custom bacterial two hybrid system to select single-domain antibodies (nanobodies) that recognize human pathogens. Informed by our Nanopore next generation sequencing of DNA samples from ambulance vehicles, antibodies targeting important pathogens were then used to develop a rapid and low cost ELISA-based testing kit that may be employed on-site by EMS workers. Our project provides a framework for rapid detection of emergent pathogens and a practical and rapid solution for monitoring their presence in and outside of the healthcare system.</p>
 +
        </div>
  
−
<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
+
        <div class="col l3 s12">
 +
          <h5 class="white-text">Our Project</h5>
 +
          <ul style="list-style-image: none;">
 +
            <li><a class="white-text" href="#!">Link 1</a></li>
 +
            <li><a class="white-text" href="#!">Link 2</a></li>
 +
            <li><a class="white-text" href="#!">Link 3</a></li>
 +
            <li><a class="white-text" href="#!">Link 4</a></li>
 +
          </ul>
 +
        </div>
 +
        <div class="col l3 s12">
 +
          <h5 class="white-text">Human Practices</h5>
 +
          <ul style="list-style-image: none;">
 +
            <li><a class="white-text" href="#!">Link 1</a></li>
 +
            <li><a class="white-text" href="#!">Link 2</a></li>
 +
            <li><a class="white-text" href="#!">Link 3</a></li>
 +
            <li><a class="white-text" href="#!">Link 4</a></li>
 +
          </ul>
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </footer>
 
</div>
 
</div>
 +
<!-- Scripts -->
  
−
 
+
  <script src="https://2016.igem.org/Template:Team:Lethbridge/jQuery2-1-1?action=raw&ctype=text/javascript" type="text/javascript"></script>
 +
  <script src="https://2016.igem.org/Template:Lethbridge/MaterializeJS?action=raw&ctype=text/javascript" type="text/javascript"></script>
 +
  <script src="https://2016.igem.org/Template:Lethbridge/InitJS?action=raw&ctype=text/javascript" type="text/javascript"></script>
 +
  </body>
 
</html>
 
</html>

Revision as of 05:41, 13 October 2016

Lethbridge iGEM 2016



Nanoresponders

Single-domain antibody production.
Explore


Introduction

The prevalence of virulent and multiple antibiotic resistant pathogens in healthcare facilities has resulted in ongoing reassessment of best practices to prevent their transmission. However, whether pathogen reservoirs exist in emergency medical services (EMS) vehicles, remains largely unknown. Our iGEM team developed a custom bacterial two hybrid system to select single-domain antibodies (nanobodies) that recognize human pathogens. Informed by our Nanopore next generation sequencing of DNA samples from ambulance vehicles, antibodies targeting important pathogens were then used to develop a rapid and low cost ELISA-based testing kit that may be employed on-site by EMS workers. Our project provides a framework for rapid detection of emergent pathogens and a practical and rapid solution for monitoring their presence in and outside of the healthcare system.

Card Title

I am a very simple card. I am good at containing small bits of information. I am convenient because I require little markup to use effectively.

This is a link This is a link
I am a very simple card. I am good at containing small bits of information. I am convenient because I require little markup to use effectively. I am similar to what is called a panel in other frameworks.
Overview

The prevalence of virulent and multiple antibiotic resistant pathogens in healthcare facilities has resulted in ongoing reassessment of best practices to prevent their transmission. However, whether pathogen reservoirs exist in emergency medical services (EMS) vehicles, remains largely unknown. Our iGEM team developed a custom bacterial two hybrid system to select single-domain antibodies (nanobodies) that recognize human pathogens. Informed by our Nanopore next generation sequencing of DNA samples from ambulance vehicles, antibodies targeting important pathogens were then used to develop a rapid and low cost ELISA-based testing kit that may be employed on-site by EMS workers. Our project provides a framework for rapid detection of emergent pathogens and a practical and rapid solution for monitoring their presence in and outside of the healthcare system.

Our Project
Human Practices