Difference between revisions of "Team:SCAU-China/Notebook"

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    <li><a href="https://2016.igem.org/Team:SCAU-China/Time_shaft">Time shaft</a></li>
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    <li><a href="https://2016.igem.org/Team:SCAU-China/Members">Members</a></li>
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    <li><a href="https://2016.igem.org/Team:SCAU-China/Advisors">Advisors</a></li>
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    <li><a href="  https://2016.igem.org/Team:SCAU-China/Attributions">Attributions  </a></li>
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    <li><a href=" https://2016.igem.org/Team:SCAU-China/Collaborations">Collaborations</a></li>
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    <li><a href="https://2016.igem.org/Team:SCAU-China/Description">Description</a></li>
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    <li><a href=" https://2016.igem.org/Team:SCAU-China/Design">Design</a></li>
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    <li><a href="https://2016.igem.org/Team:SCAU-China/Basic_Part">Basic parts</a></li>
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    <li><a href=" https://2016.igem.org/Team:SCAU-China/Experiments"> Experiments  </a></li>
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    <li><a href="https://2016.igem.org/Team:SCAU-China/Proof">Proof</a></li>
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    <li><a href="https://2016.igem.org/Team:SCAU-China/Demonstrate">Demonstrate</a></li>
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    <li><a href="https://2016.igem.org/Team:SCAU-China/Model">Model</a></li>
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    <li><a href=" https://2016.igem.org/Team:SCAU-China/Notebook">Notebook</a></li>
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    <li><a href="https://2016.igem.org/Team: SCAU-China/Gallery">Gallery</a></li>
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    <li><a href="https://2016.igem.org/Team: SCAU-China/Protocol">Protocol</a></li>
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    <li><a href="https://2016.igem.org/Team:SCAU-China/Safety">Safety</a></li>
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    <li><a href="  https://2016.igem.org/Team:SCAU-China/HP/Silver">Silver</a></li>
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    <li><a href=" https://2016.igem.org/Team:SCAU-China/HP/Gold">Gold </a></li>
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    <li><a href="https://2016.igem.org/Team:SCAU-China/Integrated_Practices">Integrated</a></li>
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    <li><a href=" https://2016.igem.org/Team:SCAU-China/Engagement">Engagement</a></li>
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    <li><a href=" https://2016.igem.org/Team:SCAU-China/Entrepreneurship">Entrepreneurship</a></li>
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      <a href="https://2016.igem.org/Team:SCAU-China" class="brand-logo" data-beloworigin="true" data-constrainwidth="false" data-activates="dropdown7">&nbsp;HOME</a>
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      <a class="right brand-logo dropdown-button" data-beloworigin="true" data-constrainwidth="false" style="cursor:pointer"  data-activates="dropdown7">HUMAN PRACTICES</a>
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<a class="right brand-logo dropdown-button" data-beloworigin="true" data-constrainwidth="false" style="cursor:pointer"  data-activates="dropdown6">SAFETY</a>
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<p> Document the dates you worked on your project.</p>
 
  
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<h5>What should this page have?</h5>
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    </div>
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<li>Chronological notes of what your team is doing.</li>
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<li> Brief descriptions of daily important events.</li>
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<li>Mention who participated in what task.</li>
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<h1>Notebook</h1>
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<h2>Design for marker free</h2>
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<p>In this part, we used a site-specific Cre/loxP recombination to delete the selective marker. To delete the selective resistance gene in transgenic rice, a marker-free element was used to assemble into four-gene multigene vector. This marker-free element was placed between two loxP sites, and consists of a HPT (hygromycin) resistance gene expression cassette and a Cre gene expression cassette controlled by anther-specific promoter (Figure 4). When Cre gene was expressed in transgenic rice anther, the Cre enzyme deleted the marker-free element between two loxP sites. <a href="#" text-decoration=underline>You can read more details, please click here! </a></p>
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<img alt="image" class="img-responsive"  align="center" src="https://static.igem.org/mediawiki/2016/e/ea/T--SCAU-China--safety1.jpg">
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<p><strong>Figure 1</strong> The schematic diagram of the marker-free element. PV4 is an anther-specific promoter that drives Cre gene expression in anther.</p>
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    <script src="https://2016.igem.org/Template:GDSYZX-United/Javascript/bootstrap_min?action=raw&ctype=text/javascript&v=3.3.6"></script>  
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<p>You can see what others teams have done to organize their notes:</p>
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<script src="https://2016.igem.org/Template:GDSYZX-United/Javascript/content_min?action=raw&ctype=text/javascript&v=1.0.0"></script>
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<script src="https://2016.igem.org/Team:SCAU-China/jsfile?action=raw&ctype=text/javascript"></script>  <!--mater-->
  
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<ul>
 
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<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
 
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<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
 
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<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
 
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<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
 
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Revision as of 08:33, 13 October 2016

SCAU

Notebook

image

Design for marker free

In this part, we used a site-specific Cre/loxP recombination to delete the selective marker. To delete the selective resistance gene in transgenic rice, a marker-free element was used to assemble into four-gene multigene vector. This marker-free element was placed between two loxP sites, and consists of a HPT (hygromycin) resistance gene expression cassette and a Cre gene expression cassette controlled by anther-specific promoter (Figure 4). When Cre gene was expressed in transgenic rice anther, the Cre enzyme deleted the marker-free element between two loxP sites. You can read more details, please click here!

image

Figure 1 The schematic diagram of the marker-free element. PV4 is an anther-specific promoter that drives Cre gene expression in anther.