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<h2>Design for marker free</h2>
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<p>In this part, we used a site-specific Cre/loxP recombination to delete the selective marker. To delete the selective resistance gene in transgenic rice, a marker-free element was used to assemble into four-gene multigene vector. This marker-free element was placed between two loxP sites, and consists of a HPT (hygromycin) resistance gene expression cassette and a Cre gene expression cassette controlled by anther-specific promoter (Figure 4). When Cre gene was expressed in transgenic rice anther, the Cre enzyme deleted the marker-free element between two loxP sites. <a href="#" text-decoration=underline>You can read more details, please click here! </a></p>
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<p><strong>Figure 1</strong> The schematic diagram of the marker-free element. PV4 is an anther-specific promoter that drives Cre gene expression in anther.</p>
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Revision as of 08:47, 13 October 2016

SCAU

Notebook

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