Difference between revisions of "Team:Lethbridge/Database"

Line 177: Line 177:
 
         <div class="col l8 s12">
 
         <div class="col l8 s12">
 
           <h1 class="white-text">Database</h1>
 
           <h1 class="white-text">Database</h1>
−
           <p class="grey-text text-lighten-4">The prevalence of virulent and multiple antibiotic resistant pathogens in healthcare facilities has resulted in ongoing reassessment of best practices to prevent their transmission. However, whether pathogen reservoirs exist in emergency medical services (EMS) vehicles, remains largely unknown. Our iGEM team developed a custom bacterial two hybrid system to select single-domain antibodies (nanobodies) that recognize human pathogens. Informed by our Nanopore next generation sequencing of DNA samples from ambulance vehicles, antibodies targeting important pathogens were then used to develop a rapid and low cost ELISA-based testing kit that may be employed on-site by EMS workers. Our project provides a framework for rapid detection of emergent pathogens and a practical and rapid solution for monitoring their presence in and outside of the healthcare system.</p>
+
           <p class="grey-text text-lighten-4">In our research, we discovered that the structure of the single-chain antibody has 3 variable regions that account for the differences in binding specificity and affinity to different epitopes. However, these 3 variable regions are not completely random. Specifically, conserved amino acid residues are found within each of the domains. Acknowledging the potential for conserved residues allows us to narrow our sample space when generating random libraries of single-chain antibodies. This greatly increases the chances of producing mutants with desirable characteristics. In order to make this data freely available, we have generated a library of single-chain antibodies using our reduced sample space. This database is available for all teams to access.</p>
 
         </div>
 
         </div>
 
       </div>
 
       </div>
Line 190: Line 190:
 
           <div class="card-content white-text">
 
           <div class="card-content white-text">
 
             <span class="card-title">Card Title</span>
 
             <span class="card-title">Card Title</span>
−
             <p>I am a very simple card. I am good at containing small bits of information.
+
             <p>Link to the database?</p>
−
            I am convenient because I require little markup to use effectively.</p>
+
 
           </div>
 
           </div>
 
           <div class="card-action">
 
           <div class="card-action">

Revision as of 19:57, 14 October 2016

Lethbridge iGEM 2016

Database

In our research, we discovered that the structure of the single-chain antibody has 3 variable regions that account for the differences in binding specificity and affinity to different epitopes. However, these 3 variable regions are not completely random. Specifically, conserved amino acid residues are found within each of the domains. Acknowledging the potential for conserved residues allows us to narrow our sample space when generating random libraries of single-chain antibodies. This greatly increases the chances of producing mutants with desirable characteristics. In order to make this data freely available, we have generated a library of single-chain antibodies using our reduced sample space. This database is available for all teams to access.

Card Title

Link to the database?

This is a link This is a link
I am a very simple card. I am good at containing small bits of information. I am convenient because I require little markup to use effectively. I am similar to what is called a panel in other frameworks.