We considered possible application of self-assembling peptides (SAPs). SAPs have a self-assembling ability because they are amphiphilic peptides and contain hydrophobic and hydrophilic residues. These electric charged amino acids interact with one another to form spontaneously antiparallel β-sheet in a physiochemical environmental condition.

In this projects, we used one of SAPs, RADA-16-I(RADARADARADARADA) and P₁₁-4 (QQRFEWEFEQQ). RADA-16-I has been established for 3-D culture of neural stem cells (NSCs) by creating nanostructures. P₁₁-4 has been designed to form fibers at low pH and have a cytocompatibility.

Recently, SAPs have been regarded as good biological material. We considered new application methods of SAPs through discussions. Finally, we decided to make a platform of technology for constructing multi-enzyme-complex by using Self-Assembling Regions (SARs) and they are covalently linked each other through disulfide bonds. They could be assemble multiple enzymes ring (Self-AssembRing).

Recently, self-assembling peptides (SAPs) have been remarked as a biological material It is necessary for enhancement of enzymatic activities in biochemistry. 自己組織化ペプチドについて 近年tissue engineeringにおいて非常に注目されてきた物質である. 酵素環状化,酵素活性の強化にはいろいろあるが… →ハイデルベルグからインスピレーションを受けた 多量体形成は Kill switchいろいろ方法あるが…体の水分を吸い取って代謝速度下げるなど

Bronze We completed the team registration. We wrote team wiki. Poster and a talk for the iGEM Jamboree are ready. We described all attributions clearly. We created and documented Parts pages. We submitted DNA samples of our new BioBrick Parts. We filled out Safety forms. We filled out Judging form.

Silver We validated our new BioBrick parts. We collaborated with other iGEM teams (Gifu, Kyoto, Nagahama, UT-tokyo, METU HS Ankara, Heidelberg) We considered that prevalence of synthetic biology

Gold We improved a previous part. We demonstrated a functional proof of concept of our project.