Difference between revisions of "Team:Wageningen UR/Notebook"
| Line 10: | Line 10: | ||
<li> | <li> | ||
| − | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/ProteinEngineering" style="border:5px solid # | + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/ProteinEngineering" style="border:5px solid #fff372"> <!--Change box color here--> |
<div class="square-menu-header"> | <div class="square-menu-header"> | ||
<img src="https://static.igem.org/mediawiki/2016/7/7f/T--Wageningen_UR--upbutton.jpg"/> | <img src="https://static.igem.org/mediawiki/2016/7/7f/T--Wageningen_UR--upbutton.jpg"/> | ||
| Line 19: | Line 19: | ||
</li> | </li> | ||
<li> | <li> | ||
| − | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/InVitroAssay" style="border:5px solid # | + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/InVitroAssay" style="border:5px solid #fff372"> <!--Change box color here--> |
<div class="square-menu-header"> | <div class="square-menu-header"> | ||
<img src="https://static.igem.org/mediawiki/2016/7/7f/T--Wageningen_UR--upbutton.jpg"/> | <img src="https://static.igem.org/mediawiki/2016/7/7f/T--Wageningen_UR--upbutton.jpg"/> | ||
| Line 28: | Line 28: | ||
</li> | </li> | ||
<li> | <li> | ||
| − | <a href=https://2016.igem.org/Team:Wageningen_UR/Notebook/VarroaIsolates style="border:5px solid # | + | <a href=https://2016.igem.org/Team:Wageningen_UR/Notebook/VarroaIsolates style="border:5px solid #fff372"> <!--Change box color here--> |
<div class="square-menu-header"> | <div class="square-menu-header"> | ||
<img src="https://static.igem.org/mediawiki/2016/7/7f/T--Wageningen_UR--upbutton.jpg"/> | <img src="https://static.igem.org/mediawiki/2016/7/7f/T--Wageningen_UR--upbutton.jpg"/> | ||
| Line 37: | Line 37: | ||
</li> | </li> | ||
<li> | <li> | ||
| − | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/Riboswitch" style="border:5px solid # | + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/Riboswitch" style="border:5px solid #fff372"> <!--Change box color here--> |
<div class="square-menu-header"> | <div class="square-menu-header"> | ||
| − | <img src="https://static.igem.org/mediawiki/2016/ | + | <img src="https://static.igem.org/mediawiki/2016/f/f4/T--Wageningen_UR--ribobutton.jpg"/> |
<h3 style="color:#6d5d31">Riboswitches</h3> <!--Change text color here--> | <h3 style="color:#6d5d31">Riboswitches</h3> <!--Change text color here--> | ||
</div> | </div> | ||
| Line 46: | Line 46: | ||
</li> | </li> | ||
<li> | <li> | ||
| − | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/QuorumSensing" style="border:5px solid # | + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/QuorumSensing" style="border:5px solid #fff372"> <!--Change box color here--> |
<div class="square-menu-header" > | <div class="square-menu-header" > | ||
<img src="https://static.igem.org/mediawiki/2016/b/b1/T--Wageningen_UR--quorumbutton.jpg"/> | <img src="https://static.igem.org/mediawiki/2016/b/b1/T--Wageningen_UR--quorumbutton.jpg"/> | ||
| Line 55: | Line 55: | ||
</li> | </li> | ||
<li> | <li> | ||
| − | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/OptogeneticKillSwitch" style="border:5px solid # | + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/OptogeneticKillSwitch" style="border:5px solid #fff372"> <!--Change box color here--> |
<div class="square-menu-header"> | <div class="square-menu-header"> | ||
<img src="https://static.igem.org/mediawiki/2016/7/7f/T--Wageningen_UR--upbutton.jpg"/> | <img src="https://static.igem.org/mediawiki/2016/7/7f/T--Wageningen_UR--upbutton.jpg"/> | ||
| Line 64: | Line 64: | ||
</li> | </li> | ||
<li> | <li> | ||
| − | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/Modelling" style="border:5px solid # | + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/Modelling" style="border:5px solid #fff372"> <!--Change box color here--> |
<div class="square-menu-header"> | <div class="square-menu-header"> | ||
<img src="https://static.igem.org/mediawiki/2016/7/7f/T--Wageningen_UR--upbutton.jpg"/> | <img src="https://static.igem.org/mediawiki/2016/7/7f/T--Wageningen_UR--upbutton.jpg"/> | ||
| Line 72: | Line 72: | ||
</a> | </a> | ||
<li> | <li> | ||
| − | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/Chitinases" style="border:5px solid # | + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/Chitinases" style="border:5px solid #fff372"> <!--Change box color here--> |
<div class="square-menu-header"> | <div class="square-menu-header"> | ||
<img src="https://static.igem.org/mediawiki/2016/7/7f/T--Wageningen_UR--upbutton.jpg"/> | <img src="https://static.igem.org/mediawiki/2016/7/7f/T--Wageningen_UR--upbutton.jpg"/> | ||
| Line 82: | Line 82: | ||
<li> | <li> | ||
| − | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/Cas9" style="border:5px solid # | + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/Cas9" style="border:5px solid #fff372"> <!--Change box color here--> |
<div class="square-menu-header"> | <div class="square-menu-header"> | ||
<img src="https://static.igem.org/mediawiki/2016/7/7f/T--Wageningen_UR--upbutton.jpg"/> | <img src="https://static.igem.org/mediawiki/2016/7/7f/T--Wageningen_UR--upbutton.jpg"/> | ||
| Line 92: | Line 92: | ||
<li> | <li> | ||
| − | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/ToxinScanner" style="border:5px solid # | + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/ToxinScanner" style="border:5px solid #fff372"> <!--Change box color here--> |
<div class="square-menu-header"> | <div class="square-menu-header"> | ||
<img src="https://static.igem.org/mediawiki/2016/7/7f/T--Wageningen_UR--upbutton.jpg"/> | <img src="https://static.igem.org/mediawiki/2016/7/7f/T--Wageningen_UR--upbutton.jpg"/> | ||
| Line 102: | Line 102: | ||
<li> | <li> | ||
| − | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/MetabolicModelling" style="border:5px solid # | + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/MetabolicModelling" style="border:5px solid #fff372"> <!--Change box color here--> |
<div class="square-menu-header"> | <div class="square-menu-header"> | ||
<img src="https://static.igem.org/mediawiki/2016/7/7f/T--Wageningen_UR--upbutton.jpg"/> | <img src="https://static.igem.org/mediawiki/2016/7/7f/T--Wageningen_UR--upbutton.jpg"/> | ||
| Line 112: | Line 112: | ||
<li> | <li> | ||
| − | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/ecoli_survival" style="border:5px solid # | + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/ecoli_survival" style="border:5px solid #fff372"> <!--Change box color here--> |
<div class="square-menu-header"> | <div class="square-menu-header"> | ||
<img src="https://static.igem.org/mediawiki/2016/7/7f/T--Wageningen_UR--upbutton.jpg"/> | <img src="https://static.igem.org/mediawiki/2016/7/7f/T--Wageningen_UR--upbutton.jpg"/> | ||
Revision as of 12:27, 17 October 2016
Find the documentation of our research here.
-
Protein Engineering
We wanted to engineer Cry3Aa to become toxic to Varroa destructor.
-
In Vitro Assay
To provide an alternative for tests on live Varroa mites, we designed an assay that uses brush border membrane vesicles.
-
Varroa isolates
We tried to find our own Varroa entomopathogen.
-
Riboswitches
To detect the presence of Varroa mites in the beehive, we made a B12 and a guanine riboswitch.
-
Population Dynamics
A quorum sensing system was characterized to regulate toxin production in BeeT.
-
Optogenetic Kill Switch
The light-induced kill switch was one of our biocontainment methods.
-
Modelling
How we modelled .
-
Chitinases
As a back-up, we wanted to use chitinases which are more harmful to Varroa destructor than honeybees.
-
Cas9-based Kill Switch
The Cas9-based kill switch was one of our biocontainment methods.
-
Toxin Scanner
We made a software tool for finding new Cry proteins toxic to Varroa destructor.
-
Metabolic Modelling
We wanted to use a metabolic model to see the effect of our BeeT distribution method.
-
Testing E. coli survival
Following the results of the Metabolic Model, we needed more data.
