Difference between revisions of "Team:HokkaidoU Japan/Overview"

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Revision as of 15:35, 17 October 2016

Team:HokkaidoU Japan - 2016.igem.org

 

Team:HokkaidoU Japan

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Project description

We considered possible application of self-assembling peptides (SAPs). SAPs have a self-assembling ability because they are amphiphilic peptides and contain hydrophobic and hydrophilic residues. These electric charged amino acids interact with one another to form spontaneously antiparallel β-sheet in a physiochemical environmental condition.
In this projects, we used one of SAPs, RADA-16-I(RADARADARADARADA) and P11-4 (QQRFEWEFEQQ).
RADA P11-4

Fig. 1. RADA16-I and P11-4 self-assemble under suitable physiochemical conditions due to the polar amino acids and hydrophobic interaction and form β-sheet.


RADA-16-I has been established for 3-D culture of neural stem cells (NSCs) by creating nanostructures. P11-4 has been designed to form fibers at low pH and have a cytocompatibility.
Recently, SAPs have been regarded as good biological material. We considered new application methods of SAPs through discussions. Finally, we decided to make a platform of technology for constructing multi-enzyme-complex by using Self-Assembling Regions (SARs) and they are covalently linked each other through disulfide bonds. They could be assemble multiple enzymes ring (Self-AssembRing).
large block

Fig. 2. Huge complex using SAP
We thought the intensity of fluorescent proteins, like GFP increased.


In the future, we will construct tool for making subunits of artificial multi-enzyme-complex by using this technology.

background

Self-Assembling Peptides

Recently, self-assembling peptides (SAPs) have been remarked as a biological nanostructures and hydrogel because SAPs are fit to biogenic and don’t have cytotoxicity. SAPs such as a scaffold for cell culturing and drug delivering in tissue engineering and medical technology. For example, RADA-16I self-assembles nanofibrous forming hydrogel and used for 3-D culture such as neural stem cells (NSCs), bones, cartilage tissues.

Enhancement of enzymatic activity

Improvement of enzymatic activities is necessary in chemical reactions. There are some way to enhance the enzymes activities such as amino acid substitution, and compartmentation of enzymes, circularization of enzymes, and multimelarization of enzymes. For example, 2014 iGEM Heidelberg team constructed covalently linked circulated enzymes by using intein and extein and increase the stability of enzymes.

achievements

Bronze

  • We completed the team registration.
  • We wrote team wiki.
  • Poster and a talk for the iGEM Jamboree are ready.
  • We described all attributions clearly.
  • We created and documented Parts pages.
  • We submitted DNA samples of our new BioBrick Parts.
  • We filled out Safety forms.
  • We filled out Judging form.

Silver

  • We validated our new BioBrick parts.
  • We collaborated with other iGEM teams (Gifu, Kyoto, Nagahama, UT-tokyo, METU HS Ankara, Heidelberg)
  • We considered that prevalence of synthetic biology

Gold

  • We improved a previous part.
  • We demonstrated a functional proof of concept of our project.