(22 intermediate revisions by the same user not shown) | |||
Line 6: | Line 6: | ||
<title>WATTS-APTAMER - UP_PRETORIA iGEM</title> | <title>WATTS-APTAMER - UP_PRETORIA iGEM</title> | ||
<meta content='width=device-width, initial-scale=1.0, maximum-scale=1.0, user-scalable=0' name='viewport' /> | <meta content='width=device-width, initial-scale=1.0, maximum-scale=1.0, user-scalable=0' name='viewport' /> | ||
− | <meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1" /> | + | <meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1" /> |
+ | <style> | ||
+ | #groupparts { | ||
+ | margin: auto; | ||
+ | /*width: 100% !important;*/ | ||
+ | } | ||
+ | </style> | ||
</head> | </head> | ||
<body class="landing-page"> | <body class="landing-page"> | ||
<div class="wrapper"> | <div class="wrapper"> | ||
− | |||
<div class="section section-we-made-1 section-with-hover"> | <div class="section section-we-made-1 section-with-hover"> | ||
<div class="text-area"> | <div class="text-area"> | ||
<div class="title add-animation animate"> | <div class="title add-animation animate"> | ||
<p class="h2WM">Parts</p> | <p class="h2WM">Parts</p> | ||
− | <p>This year we submitted | + | <p>This year we submitted 17 parts to the iGEM registry. Browse them below. |
</p> | </p> | ||
− | <groupparts> | + | </div> |
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | <div class="row"> | ||
+ | <div class="col-sm-12"> | ||
+ | <p style="text-align:center;"> | ||
+ | </html> | ||
+ | <groupparts>iGEM2016 Pretoria_UP</groupparts> | ||
+ | <html> | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
− | + | <div class="row" id="projectsLine1"> | |
<div class="col-md-4"> | <div class="col-md-4"> | ||
<div class="project animation-1 add-animation animate"> | <div class="project animation-1 add-animation animate"> | ||
− | <img alt="..." src="https://static.igem.org/mediawiki/2016/ | + | <img alt="..." src="https://static.igem.org/mediawiki/2016/5/57/T--Pretoria_UP--Basic_Parts.jpg"> |
− | <a class="over-area color- | + | <a class="over-area color-black" href="javascript:void(0)" onclick="rubik.showModal(this)" data-target="basic_parts"> |
<div class="content"> | <div class="content"> | ||
− | <h4> | + | <h4>Basic Parts</h4> |
<p>Click for details.</p> | <p>Click for details.</p> | ||
</div> | </div> | ||
Line 34: | Line 51: | ||
<div class="col-md-4"> | <div class="col-md-4"> | ||
<div class="project animation-2 add-animation animate"> | <div class="project animation-2 add-animation animate"> | ||
− | <img alt="..." src="https://static.igem.org/mediawiki/2016/ | + | <img alt="..." src="https://static.igem.org/mediawiki/2016/8/87/T--Pretoria_UP--Composite_Parts.jpg"> |
− | <a class="over-area color- | + | <a class="over-area color-green" href="javascript:void(0)" onclick="rubik.showModal(this)" data-target="composite_parts"> |
<div class="content"> | <div class="content"> | ||
− | <h4> | + | <h4>Composite Parts</h4> |
<p>Click for details.</p> | <p>Click for details.</p> | ||
</div> | </div> | ||
Line 45: | Line 62: | ||
<div class="col-md-4"> | <div class="col-md-4"> | ||
<div class="project animation-3 add-animation animate"> | <div class="project animation-3 add-animation animate"> | ||
− | <img alt="..." src="https://static.igem.org/mediawiki/2016/ | + | <img alt="..." src="https://static.igem.org/mediawiki/2016/e/e0/T--Pretoria_UP--Improving_Part.jpg"> |
− | <a class="over-area color-blue" href="javascript:void(0)" onclick="rubik.showModal(this)" data-target=" | + | <a class="over-area color-blue" href="javascript:void(0)" onclick="rubik.showModal(this)" data-target="improved_part"> |
<div class="content"> | <div class="content"> | ||
− | <h4> | + | <h4>Improved Characterization</h4> |
<p>Click for details.</p> | <p>Click for details.</p> | ||
</div> | </div> | ||
Line 55: | Line 72: | ||
</div> | </div> | ||
</div> | </div> | ||
− | </div>section section-we-made-1 section-with-hover--> | + | </div><!--section section-we-made-1 section-with-hover--> |
− | + | <div class="project-content" id="basic_parts"> | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
<div> | <div> | ||
<div class="project-details"> | <div class="project-details"> | ||
Line 91: | Line 80: | ||
<div class="container"> | <div class="container"> | ||
<div class="project-title"> | <div class="project-title"> | ||
− | <p class = "h2WM" style="text-align:left;"> | + | <p class = "h2WM" style="text-align:left;">Basic Parts</p> |
</div> | </div> | ||
<div class="row"> | <div class="row"> | ||
<div class="col-md-12"> | <div class="col-md-12"> | ||
<div class="project-text"> | <div class="project-text"> | ||
− | <p> | + | <p>We added 5 new basic parts. An SP6 promoter which can be used for in vitro expression of genes, as well as 4 eukaryotic Laccase proteins from <i>Eucalyptus grandis</i>. |
+ | </p> | ||
+ | <br> | ||
+ | <p style="text-align:left;"> | ||
+ | <a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037000">K2037000</a>: An promoter from bacteriophage SP6 that is recognised by an SP6 RNA Polymerase. This part can aid in in vitro expression of various genes. | ||
+ | <br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037001">K2037001</a>: Laccase 'EucgrA01282' protein from Eucalyptus grandis with a His (x6) tag after the start codon. | ||
+ | <br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037002">K2037002</a>: Laccase 'EucgrB02797' protein from Eucalyptus grandis with His(x6) tag after start codon. | ||
+ | <br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037003">K2037003</a>: Laccase 'EucgrG03098' protein from Eucalyptus grandis with His (x6) tag after start codon. | ||
+ | <br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037004">K2037004</a>: Laccase 'EucgrF02641' protein from Eucalyptus grandis with His (x6) tag after start codon. | ||
</p> | </p> | ||
<p></p> | <p></p> | ||
Line 110: | Line 107: | ||
</div> | </div> | ||
</div> | </div> | ||
− | </div>project-content--> | + | </div><!--project-content--> |
− | + | <div class="project-content" id="composite_parts"> | |
<div> | <div> | ||
<div class="project-details"> | <div class="project-details"> | ||
Line 117: | Line 114: | ||
<div class="container"> | <div class="container"> | ||
<div class="project-title"> | <div class="project-title"> | ||
− | <p class = "h2WM" style="text-align:left;"> | + | <p class = "h2WM" style="text-align:left;">Composite Parts</p> |
</div> | </div> | ||
<div class="row"> | <div class="row"> | ||
<div class="col-md-12"> | <div class="col-md-12"> | ||
<div class="project-text"> | <div class="project-text"> | ||
− | <p>We | + | <p>We designed and added 12 new composite parts to the registry. Four eukaryotic laccase genes from <i>Eucalyptus grandis</i>, as well eight prokaryotic laccase genes, all of which has an SP6 promoter and a His(x6) tag at the 5’ end and a T7 terminator at the 3’ end. |
+ | </p> | ||
+ | <br> | ||
+ | <p style="text-align:left;"> | ||
+ | <a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037005">K2037005</a>: SP6 promoter attached to Laccase 'EucgrA01282' protein sequence with a His (6x) tag after the start codon. T7 terminator at the end. | ||
+ | <br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037006">K2037006</a>: SP6 promoter attached to Laccase 'EucgrB02797' protein sequence with a His (6x) tag after the start codon. T7 terminator at the end. | ||
+ | <br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037007">K2037007</a>: SP6 promoter attached to Laccase 'EucgrG03098' protein sequence with a His (6x) tag after the start codon. T7 terminator at the end. | ||
+ | <br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037008">K2037008</a>: SP6 promoter attached to Laccase 'EucgrF02641' protein sequence with a His (6x) tag after the start codon. T7 terminator at the end. | ||
+ | <br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037009">K2037009</a>: SP6 promoter attached to Laccase from Bacillus pumilus with His(6x) tag. T7 terminator at the end. | ||
+ | <br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037010">K2037010</a>: SP6 promoter attached to Laccase from Bacillus pumilus with His(6x) tag. T7 terminator at the end. | ||
+ | <br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037012">K2037012</a>: Part K863005 with an SP6 promoter added as well as a T7 terminator. | ||
+ | <br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037013">K2037013</a>: Part K863006 with an SP6 promoter added as well as a T7 terminator. | ||
+ | <br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037014">K2037014</a>: SP6 promoter joined to a tthl laccase from Thermus thermophilus with T7 promoter, RBS and His-tag. | ||
+ | <br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037015">K2037015</a>: SP6 promoter Joined to tthl laccase from Thermus thermophiles and a T7 terminator. | ||
+ | <br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037016">K2037016</a>: SP6 promoter combined with bhal laccase from Bacillus halodurans with T7 promoter, RBS and His-tag and a T7 terminator. | ||
+ | <br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037017">K2037017</a>: SP6 promoter joined to bhal laccase from Bacillus halodurans joined to a T7 terminator. | ||
</p> | </p> | ||
<p></p> | <p></p> | ||
Line 136: | Line 148: | ||
</div> | </div> | ||
</div> | </div> | ||
− | </div>project-content--> | + | </div><!--project-content--> |
− | + | <div class="project-content" id="improved_part"> | |
<div> | <div> | ||
<div class="project-details"> | <div class="project-details"> | ||
Line 143: | Line 155: | ||
<div class="container"> | <div class="container"> | ||
<div class="project-title"> | <div class="project-title"> | ||
− | <p class = "h2WM" style="text-align:left;"> | + | <p class = "h2WM" style="text-align:left;">Improved Characterization of a Part</p> |
</div> | </div> | ||
<div class="row"> | <div class="row"> | ||
<div class="col-md-12"> | <div class="col-md-12"> | ||
<div class="project-text"> | <div class="project-text"> | ||
− | <p> | + | <p>Two parts were chosen to improve the characterization. Both of these parts code for laccase proteins and were lacking a thorough characterization in the registry. |
+ | </p> | ||
+ | <p><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K863010">BBa_K863010</a>: tthl (Laccase from Thermus thermophilus) with T7, RBS and HIS tag. | ||
+ | <br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K863020">BBa_K863020</a>: bhal laccase from Bacillus halodurans with T7 and HIS. | ||
</p> | </p> | ||
<p></p> | <p></p> | ||
Line 162: | Line 177: | ||
</div> | </div> | ||
</div> | </div> | ||
− | </div>project-content--> | + | </div><!--project-content--> |
</div><!--wrapper--> | </div><!--wrapper--> | ||
</body> | </body> | ||
</html> | </html> | ||
{{:Team:Pretoria_UP/Footer}} | {{:Team:Pretoria_UP/Footer}} |
Latest revision as of 09:30, 18 October 2016
Parts This year we submitted 17 parts to the iGEM registry. Browse them below.
<groupparts>iGEM2016 Pretoria_UP</groupparts>
Basic Parts We added 5 new basic parts. An SP6 promoter which can be used for in vitro expression of genes, as well as 4 eukaryotic Laccase proteins from Eucalyptus grandis.
K2037000: An promoter from bacteriophage SP6 that is recognised by an SP6 RNA Polymerase. This part can aid in in vitro expression of various genes.
Composite Parts We designed and added 12 new composite parts to the registry. Four eukaryotic laccase genes from Eucalyptus grandis, as well eight prokaryotic laccase genes, all of which has an SP6 promoter and a His(x6) tag at the 5’ end and a T7 terminator at the 3’ end.
K2037005: SP6 promoter attached to Laccase 'EucgrA01282' protein sequence with a His (6x) tag after the start codon. T7 terminator at the end.
K2037001: Laccase 'EucgrA01282' protein from Eucalyptus grandis with a His (x6) tag after the start codon.
K2037002: Laccase 'EucgrB02797' protein from Eucalyptus grandis with His(x6) tag after start codon.
K2037003: Laccase 'EucgrG03098' protein from Eucalyptus grandis with His (x6) tag after start codon.
K2037004: Laccase 'EucgrF02641' protein from Eucalyptus grandis with His (x6) tag after start codon.
K2037006: SP6 promoter attached to Laccase 'EucgrB02797' protein sequence with a His (6x) tag after the start codon. T7 terminator at the end.
K2037007: SP6 promoter attached to Laccase 'EucgrG03098' protein sequence with a His (6x) tag after the start codon. T7 terminator at the end.
K2037008: SP6 promoter attached to Laccase 'EucgrF02641' protein sequence with a His (6x) tag after the start codon. T7 terminator at the end.
K2037009: SP6 promoter attached to Laccase from Bacillus pumilus with His(6x) tag. T7 terminator at the end.
K2037010: SP6 promoter attached to Laccase from Bacillus pumilus with His(6x) tag. T7 terminator at the end.
K2037012: Part K863005 with an SP6 promoter added as well as a T7 terminator.
K2037013: Part K863006 with an SP6 promoter added as well as a T7 terminator.
K2037014: SP6 promoter joined to a tthl laccase from Thermus thermophilus with T7 promoter, RBS and His-tag.
K2037015: SP6 promoter Joined to tthl laccase from Thermus thermophiles and a T7 terminator.
K2037016: SP6 promoter combined with bhal laccase from Bacillus halodurans with T7 promoter, RBS and His-tag and a T7 terminator.
K2037017: SP6 promoter joined to bhal laccase from Bacillus halodurans joined to a T7 terminator.
Improved Characterization of a Part
Two parts were chosen to improve the characterization. Both of these parts code for laccase proteins and were lacking a thorough characterization in the registry.
BBa_K863010: tthl (Laccase from Thermus thermophilus) with T7, RBS and HIS tag.
BBa_K863020: bhal laccase from Bacillus halodurans with T7 and HIS.