Difference between revisions of "Team:Pretoria UP/Parts"

 
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<title>WATTS-APTAMER - UP_PRETORIA iGEM</title>
 
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<p class="h2WM">Parts</p>
 
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<p>This year we submitted 18 parts to the iGEM registry. Browse them below.  
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<p>This year we submitted 17 parts to the iGEM registry. Browse them below.  
 
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<groupparts>iGEM2016 Pretoria_UP</groupparts>
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<groupparts>iGEM2016 Pretoria_UP</groupparts>
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<img alt="..." src="https://static.igem.org/mediawiki/2016/d/d6/T--Pretoria_UP--Collab_1.jpg">
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<h4>Aix-Marseille University</h4>
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<h4>Basic Parts</h4>
 
<p>Click for details.</p>
 
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<h4>Macquarie University</h4>
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<h4>Composite Parts</h4>
 
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<h4>Heidelberg University</h4>
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<h4>Improved Characterization</h4>
 
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<p><b>References</b>
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1. Study Ranger n.d., <i>Université de la Méditerranée Aix-Marseille II</i>, viewed 06 October 2016, available at https://www.studyranger.com/en/company/12004.
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2. Universities Australia 2015, <i>Macquarie University</i>, viewed 06 October 2016, available at https://www.universitiesaustralia.edu.au/australias-universities/university-profiles/Macquarie-University#.V_ZvwOB97IU.
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3. Beckendorf, J 2003, <i>Universitätsbibliothek Heidelberg</i>, viewed 06 October 2016, available at https://en.wikipedia.org/wiki/File:Heidelberg_Universit%C3%A4tsbibliothek_2003.jpg.
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<p class = "h2WM" style="text-align:left;">Collaboration with Aix-Marseille University</p>
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<p class = "h2WM" style="text-align:left;">Basic Parts</p>
 
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<p>South Africa is regarded as having the largest platinum reserves in the world. The Merensky Reef stretching from southern Zimbabwe through to Rustenburg and Pretoria is the centre of platinum mining in South Africa. AMPLATS is the leader in platinum mining in South Africa, producing 40% of the total group platinum group metals. The Aix-Marseille University iGEM team identified problems faced by the mining sector which include limited sources, the use of hazardous chemicals to eliminate impurities, the disregard of safety measures and the lack of methods to recycle platinum next to highways.The solutions they came up with are to concentrate the platinum accumulated from roadside soil by phytoremediation plants and to transform it into nanoparticles. We assisted them by creating a documentary focusing on the socio-economic and political issues facing the current platinum sector, including the Marikana strikes. We also looked at the possible implications of their 2016 project.
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<p>We added 5 new basic parts. An SP6 promoter which can be used for in vitro expression of genes, as well as 4 eukaryotic Laccase proteins from <i>Eucalyptus grandis</i>.
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<a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037000">K2037000</a>: An promoter from bacteriophage SP6 that is recognised by an SP6 RNA Polymerase. This part can aid in in vitro expression of various genes.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037001">K2037001</a>: Laccase 'EucgrA01282' protein from Eucalyptus grandis with a His (x6) tag after the start codon.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037002">K2037002</a>: Laccase 'EucgrB02797' protein from Eucalyptus grandis with His(x6) tag after start codon.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037003">K2037003</a>: Laccase 'EucgrG03098' protein from Eucalyptus grandis with His (x6) tag after start codon.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037004">K2037004</a>: Laccase 'EucgrF02641' protein from Eucalyptus grandis with His (x6) tag after start codon.
 
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<p class = "h2WM" style="text-align:left;">Collaboration with Macquarie University</p>
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<p class = "h2WM" style="text-align:left;">Composite Parts</p>
 
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<p>We assisted the iGEM team, MacQuarrie, by making a graphical user interface on MatLab® showing how the concentration of various intermediates, including ALA, (Y-axis) changes over time (X-axis) in chlorophyll. Here is the interactive graph we helped them with:
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<p>We designed and added 12 new composite parts to the registry. Four eukaryotic laccase genes from <i>Eucalyptus grandis</i>, as well eight prokaryotic laccase genes, all of which has an SP6 promoter and a His(x6) tag at the 5’ end and a T7 terminator at the 3’ end.
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<a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037005">K2037005</a>: SP6 promoter attached to Laccase 'EucgrA01282' protein sequence with a His (6x) tag after the start codon. T7 terminator at the end.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037006">K2037006</a>: SP6 promoter attached to Laccase 'EucgrB02797' protein sequence with a His (6x) tag after the start codon. T7 terminator at the end.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037007">K2037007</a>: SP6 promoter attached to Laccase 'EucgrG03098' protein sequence with a His (6x) tag after the start codon. T7 terminator at the end.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037008">K2037008</a>: SP6 promoter attached to Laccase 'EucgrF02641' protein sequence with a His (6x) tag after the start codon. T7 terminator at the end.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037009">K2037009</a>: SP6 promoter attached to Laccase from Bacillus pumilus with His(6x) tag. T7 terminator at the end.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037010">K2037010</a>: SP6 promoter attached to Laccase from Bacillus pumilus with His(6x) tag. T7 terminator at the end.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037012">K2037012</a>: Part K863005 with an SP6 promoter added as well as a T7 terminator.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037013">K2037013</a>: Part K863006 with an SP6 promoter added as well as a T7 terminator.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037014">K2037014</a>: SP6 promoter joined to a tthl laccase from Thermus thermophilus with T7 promoter, RBS and His-tag.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037015">K2037015</a>: SP6 promoter Joined to tthl laccase from Thermus thermophiles and a T7 terminator.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037016">K2037016</a>: SP6 promoter combined with bhal laccase from Bacillus halodurans with T7 promoter, RBS and His-tag and a T7 terminator.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037017">K2037017</a>: SP6 promoter joined to bhal laccase from Bacillus halodurans joined to a T7 terminator.
 
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<p class = "h2WM" style="text-align:left;">Improved Characterization of a Part</p>
 
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<p>Two parts were chosen to improve the characterization. Both of these parts code for laccase proteins and were lacking a thorough characterization in the registry.
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<p><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K863010">BBa_K863010</a>: tthl (Laccase from Thermus thermophilus) with T7, RBS and HIS tag.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K863020">BBa_K863020</a>: bhal laccase from Bacillus halodurans with T7 and HIS.
 
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Latest revision as of 09:30, 18 October 2016

WATTS-APTAMER - PRETORIA_UP iGEM

WATTS-APTAMER - UP_PRETORIA iGEM

Parts

This year we submitted 17 parts to the iGEM registry. Browse them below.

<groupparts>iGEM2016 Pretoria_UP</groupparts>

Basic Parts

We added 5 new basic parts. An SP6 promoter which can be used for in vitro expression of genes, as well as 4 eukaryotic Laccase proteins from Eucalyptus grandis.


K2037000: An promoter from bacteriophage SP6 that is recognised by an SP6 RNA Polymerase. This part can aid in in vitro expression of various genes.
K2037001: Laccase 'EucgrA01282' protein from Eucalyptus grandis with a His (x6) tag after the start codon.
K2037002: Laccase 'EucgrB02797' protein from Eucalyptus grandis with His(x6) tag after start codon.
K2037003: Laccase 'EucgrG03098' protein from Eucalyptus grandis with His (x6) tag after start codon.
K2037004: Laccase 'EucgrF02641' protein from Eucalyptus grandis with His (x6) tag after start codon.

Composite Parts

We designed and added 12 new composite parts to the registry. Four eukaryotic laccase genes from Eucalyptus grandis, as well eight prokaryotic laccase genes, all of which has an SP6 promoter and a His(x6) tag at the 5’ end and a T7 terminator at the 3’ end.


K2037005: SP6 promoter attached to Laccase 'EucgrA01282' protein sequence with a His (6x) tag after the start codon. T7 terminator at the end.
K2037006: SP6 promoter attached to Laccase 'EucgrB02797' protein sequence with a His (6x) tag after the start codon. T7 terminator at the end.
K2037007: SP6 promoter attached to Laccase 'EucgrG03098' protein sequence with a His (6x) tag after the start codon. T7 terminator at the end.
K2037008: SP6 promoter attached to Laccase 'EucgrF02641' protein sequence with a His (6x) tag after the start codon. T7 terminator at the end.
K2037009: SP6 promoter attached to Laccase from Bacillus pumilus with His(6x) tag. T7 terminator at the end.
K2037010: SP6 promoter attached to Laccase from Bacillus pumilus with His(6x) tag. T7 terminator at the end.
K2037012: Part K863005 with an SP6 promoter added as well as a T7 terminator.
K2037013: Part K863006 with an SP6 promoter added as well as a T7 terminator.
K2037014: SP6 promoter joined to a tthl laccase from Thermus thermophilus with T7 promoter, RBS and His-tag.
K2037015: SP6 promoter Joined to tthl laccase from Thermus thermophiles and a T7 terminator.
K2037016: SP6 promoter combined with bhal laccase from Bacillus halodurans with T7 promoter, RBS and His-tag and a T7 terminator.
K2037017: SP6 promoter joined to bhal laccase from Bacillus halodurans joined to a T7 terminator.

Improved Characterization of a Part

Two parts were chosen to improve the characterization. Both of these parts code for laccase proteins and were lacking a thorough characterization in the registry.

BBa_K863010: tthl (Laccase from Thermus thermophilus) with T7, RBS and HIS tag.
BBa_K863020: bhal laccase from Bacillus halodurans with T7 and HIS.

WATTS-APTAMER - PRETORIA_UP iGEM