Difference between revisions of "Team:Pretoria UP/Parts"

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<title>WATTS-APTAMER - UP_PRETORIA iGEM</title>
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<p class="h2WM">Parts</p>
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<p>This year we submitted 17 parts to the iGEM registry. Browse them below.
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<h5>Note</h5>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<groupparts>iGEM2016 Pretoria_UP</groupparts>
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<h5>Adding parts to the registry</h5>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<img alt="..." src="https://static.igem.org/mediawiki/2016/5/57/T--Pretoria_UP--Basic_Parts.jpg">
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<h5>What information do I need to start putting my parts on the Registry?</h5>
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<h4>Basic Parts</h4>
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<p>The information needed to initially create a part on the Registry is:</p>
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<p>Click for details.</p>
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<li>Part Name</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<img alt="..." src="https://static.igem.org/mediawiki/2016/8/87/T--Pretoria_UP--Composite_Parts.jpg">
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<li>Design considerations</li>
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<h4>Composite Parts</h4>
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<p>Click for details.</p>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h5>Inspiration</h5>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<h4>Improved Characterization</h4>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<p>Click for details.</p>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<h5>Part Table </h5>
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<groupparts>iGEM2016 Example</groupparts>
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<p>We added 5 new basic parts. An SP6 promoter which can be used for in vitro expression of genes, as well as 4 eukaryotic Laccase proteins from <i>Eucalyptus grandis</i>.
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<a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037000">K2037000</a>: An promoter from bacteriophage SP6 that is recognised by an SP6 RNA Polymerase. This part can aid in in vitro expression of various genes.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037001">K2037001</a>: Laccase 'EucgrA01282' protein from Eucalyptus grandis with a His (x6) tag after the start codon.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037002">K2037002</a>: Laccase 'EucgrB02797' protein from Eucalyptus grandis with His(x6) tag after start codon.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037003">K2037003</a>: Laccase 'EucgrG03098' protein from Eucalyptus grandis with His (x6) tag after start codon.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037004">K2037004</a>: Laccase 'EucgrF02641' protein from Eucalyptus grandis with His (x6) tag after start codon.
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<p>We designed and added 12 new composite parts to the registry. Four eukaryotic laccase genes from <i>Eucalyptus grandis</i>, as well eight prokaryotic laccase genes, all of which has an SP6 promoter and a His(x6) tag at the 5’ end and a T7 terminator at the 3’ end.
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<a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037005">K2037005</a>: SP6 promoter attached to Laccase 'EucgrA01282' protein sequence with a His (6x) tag after the start codon. T7 terminator at the end.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037006">K2037006</a>: SP6 promoter attached to Laccase 'EucgrB02797' protein sequence with a His (6x) tag after the start codon. T7 terminator at the end.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037007">K2037007</a>: SP6 promoter attached to Laccase 'EucgrG03098' protein sequence with a His (6x) tag after the start codon. T7 terminator at the end.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037008">K2037008</a>: SP6 promoter attached to Laccase 'EucgrF02641' protein sequence with a His (6x) tag after the start codon. T7 terminator at the end.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037009">K2037009</a>: SP6 promoter attached to Laccase from Bacillus pumilus with His(6x) tag. T7 terminator at the end.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037010">K2037010</a>: SP6 promoter attached to Laccase from Bacillus pumilus with His(6x) tag. T7 terminator at the end.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037012">K2037012</a>: Part K863005 with an SP6 promoter added as well as a T7 terminator.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037013">K2037013</a>: Part K863006 with an SP6 promoter added as well as a T7 terminator.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037014">K2037014</a>: SP6 promoter joined to a tthl laccase from Thermus thermophilus with T7 promoter, RBS and His-tag.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037015">K2037015</a>: SP6 promoter Joined to tthl laccase from Thermus thermophiles and a T7 terminator.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037016">K2037016</a>: SP6 promoter combined with bhal laccase from Bacillus halodurans with T7 promoter, RBS and His-tag and a T7 terminator.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K2037017">K2037017</a>: SP6 promoter joined to bhal laccase from Bacillus halodurans joined to a T7 terminator.
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<p>Two parts were chosen to improve the characterization. Both of these parts code for laccase proteins and were lacking a thorough characterization in the registry.
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<p><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K863010">BBa_K863010</a>: tthl (Laccase from Thermus thermophilus) with T7, RBS and HIS tag.
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<br><a style="color: #c6c6c6 !important;" href="http://parts.igem.org/Part:BBa_K863020">BBa_K863020</a>: bhal laccase from Bacillus halodurans with T7 and HIS.
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{{:Team:Pretoria_UP/Footer}}

Latest revision as of 09:30, 18 October 2016

WATTS-APTAMER - PRETORIA_UP iGEM

WATTS-APTAMER - UP_PRETORIA iGEM

Parts

This year we submitted 17 parts to the iGEM registry. Browse them below.

<groupparts>iGEM2016 Pretoria_UP</groupparts>

Basic Parts

We added 5 new basic parts. An SP6 promoter which can be used for in vitro expression of genes, as well as 4 eukaryotic Laccase proteins from Eucalyptus grandis.


K2037000: An promoter from bacteriophage SP6 that is recognised by an SP6 RNA Polymerase. This part can aid in in vitro expression of various genes.
K2037001: Laccase 'EucgrA01282' protein from Eucalyptus grandis with a His (x6) tag after the start codon.
K2037002: Laccase 'EucgrB02797' protein from Eucalyptus grandis with His(x6) tag after start codon.
K2037003: Laccase 'EucgrG03098' protein from Eucalyptus grandis with His (x6) tag after start codon.
K2037004: Laccase 'EucgrF02641' protein from Eucalyptus grandis with His (x6) tag after start codon.

Composite Parts

We designed and added 12 new composite parts to the registry. Four eukaryotic laccase genes from Eucalyptus grandis, as well eight prokaryotic laccase genes, all of which has an SP6 promoter and a His(x6) tag at the 5’ end and a T7 terminator at the 3’ end.


K2037005: SP6 promoter attached to Laccase 'EucgrA01282' protein sequence with a His (6x) tag after the start codon. T7 terminator at the end.
K2037006: SP6 promoter attached to Laccase 'EucgrB02797' protein sequence with a His (6x) tag after the start codon. T7 terminator at the end.
K2037007: SP6 promoter attached to Laccase 'EucgrG03098' protein sequence with a His (6x) tag after the start codon. T7 terminator at the end.
K2037008: SP6 promoter attached to Laccase 'EucgrF02641' protein sequence with a His (6x) tag after the start codon. T7 terminator at the end.
K2037009: SP6 promoter attached to Laccase from Bacillus pumilus with His(6x) tag. T7 terminator at the end.
K2037010: SP6 promoter attached to Laccase from Bacillus pumilus with His(6x) tag. T7 terminator at the end.
K2037012: Part K863005 with an SP6 promoter added as well as a T7 terminator.
K2037013: Part K863006 with an SP6 promoter added as well as a T7 terminator.
K2037014: SP6 promoter joined to a tthl laccase from Thermus thermophilus with T7 promoter, RBS and His-tag.
K2037015: SP6 promoter Joined to tthl laccase from Thermus thermophiles and a T7 terminator.
K2037016: SP6 promoter combined with bhal laccase from Bacillus halodurans with T7 promoter, RBS and His-tag and a T7 terminator.
K2037017: SP6 promoter joined to bhal laccase from Bacillus halodurans joined to a T7 terminator.

Improved Characterization of a Part

Two parts were chosen to improve the characterization. Both of these parts code for laccase proteins and were lacking a thorough characterization in the registry.

BBa_K863010: tthl (Laccase from Thermus thermophilus) with T7, RBS and HIS tag.
BBa_K863020: bhal laccase from Bacillus halodurans with T7 and HIS.

WATTS-APTAMER - PRETORIA_UP iGEM