Difference between revisions of "Team:HokkaidoU Japan/Overview"
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Revision as of 23:54, 18 October 2016
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Team:HokkaidoU Japan
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We focused on possible application of self-assembling peptides (SAPs). SAPs have a self-assembling ability because they are amphiphilic peptides and contain hydrophobic and hydrophilic residues. These electric charged amino acids interact with one another to form spontaneously antiparallel β-sheet in a physiochemical environmental condition.
In this projects, we used one of SAPs, RADA16-I (RADARADARADARADA) and P11-4 (QQRFEWEFEQQ).
RADA16-I has been established for 3-D culture of neural stem cells (NSCs) by creating nanostructures. P11-4 has been designed to form fibers at low pH and have a cytocompatibility.
Recently, SAPs have proved a good biological material. We investigated new application methods of SAPs through discussions. Finally, we decided to construct multi-enzyme-complex platform using SAPs and short linker (SL). They are covalently linked each other through disulfide bonds and may constitute a circularized multiple enzymes(Self-AssembRing).
In the future, we will construct a tool for making subunits of artificial multi-enzyme-complex by using this technology.
We focused on possible application of self-assembling peptides (SAPs). SAPs have a self-assembling ability because they are amphiphilic peptides and contain hydrophobic and hydrophilic residues. These electric charged amino acids interact with one another to form spontaneously antiparallel β-sheet in a physiochemical environmental condition.
In this projects, we used one of SAPs, RADA16-I (RADARADARADARADA) and P11-4 (QQRFEWEFEQQ).
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Fig. 1. RADA16-I and P11-4 self-assemble under suitable physiochemical conditions due to the polar amino acids and hydrophobic interaction and form β-sheet.
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Fig. 2. Forming multiple GFPs ring (Self-AssembRing) |
RADA16-I has been established for 3-D culture of neural stem cells (NSCs) by creating nanostructures. P11-4 has been designed to form fibers at low pH and have a cytocompatibility.
Recently, SAPs have proved a good biological material. We investigated new application methods of SAPs through discussions. Finally, we decided to construct multi-enzyme-complex platform using SAPs and short linker (SL). They are covalently linked each other through disulfide bonds and may constitute a circularized multiple enzymes(Self-AssembRing).
In the future, we will construct a tool for making subunits of artificial multi-enzyme-complex by using this technology.
Self-Assembling Peptides
Recently, self-assembling peptides (SAPs) have been noted as biological nanostructures and hydrogel because SAPs are provided with biogenic suitabilities and don’t have cytotoxicity. SAPs have been used as a scaffold for cell culturing and drug delivering in tissue engineering and medical technology. For example, RADA16-I self-assembles nanofibrous forming hydrogel and is used for 3-D culture such as neural stem cells (NSCs), bones, cartilage tissues.Enhancement of enzymatic activity
Improving enzymatic activities is needed promote chemical reactions. Enzymatic activities can be enhanced by various ways including amino acid substitution, enzyme compartmentation, circularization, and multimelarization. For example, 2014 iGEM Heidelberg team constructed covalently linked circulated enzymes by using intein and extein to increase the enzyme stability.Bronze
- We completed the team registration.
- We wrote team wiki.
- Poster and a talk for the iGEM Jamboree are ready.
- We described all attributions clearly.
- We created and documented Parts pages.
- We submitted DNA samples of our new BioBrick Parts.
- We filled out Safety forms.
- We filled out Judging form.
Silver
- We validated our new BioBrick parts.
- We collaborated with other iGEM teams (Gifu, Kyoto, Nagahama, UT-tokyo, METU HS Ankara, Heidelberg)
- We considered that prevalence of synthetic biology
Gold
- We improved a previous part.
- We demonstrated a functional proof of concept of our project.
