JUDGING CRITERIA

In fulfillment of the requirements for the Gold Medal, the 2016 Wageningen iGEM Team did the following:

Bronze Medal Requirements：

1. Registered for iGEM and ready to present our project at the 2016 Giant Jamboree.
2. Created and submitted to the Registry 31 new BioBricks
3. Created a documented attributions page in our wiki.
4. Completed the Safety and Judging forms and met all other required deliverables.

Silver Medal Requirements：

1. Validated the functionality of several of the submitted BioBricks.
• BBa_K1913005 encodes a quorum sensing system with GFP as reporter. We characterized this BioBrick showing its functionality.
• BBa_K1913008, BBa_K1913009 are a Vitamin B12 and a Guanine riboswitch, respectively. Both of them were functionally validated in vivo using RFP as reporter.
• BBa_K1913015 encodes a Cry3Aa1 like protein that has a high level of toxicity towards most kind of insects. We experimentally validated its toxicity in vitro by using brush border membrane vesicles (BBMV).
• FixK2/plac hybrid promoters (BBa_K1913025, BBa_K1913026) were funcionally vali-dated in combination with light sensor generator (BBa_K1913034).
2. Considered safety in our project by implementing a light Kill Switch system and a synthetic amino acid system to prevent horizontal gene transfer. By implementing these systems, we will reduce the environmental impact of BeeT.
3. Collaborated with the TU_Delft and Groningen iGEM teams.
4. As human practice activities, we discussed our project application scenario with Synenergene and we wrote in collaboration with them a future-scenario. We also organized the national Dutch conference with other iGEM teams to share our experiences.

Gold Medal Requirements:

1. Improve and verify several previous parts:
• We improved the characterization of BBa_K546000, a quorum sensing composite part that we tested together with BBa_K1913005 getting these fluorescence graphs.
• Based on the original FixK2 promoter (BBa_K592006 made by Uppsala-Sweden_2011), we designed and provided new FixK2 promoters by adding a core control element and operons to the canoni-cal FixJ binding sites, making them controllable by different inputs signal.
2. Integrated Human Practices:

Integrated Human Practices: We consulted several stakeholders and specialist such as beekeepers from the Dutch Beekeeper association, specialists from the Wageningen University to get suggestions in order to make our BeeT product more feasible and acceptable. According to their suggestions, our product should fit with the hobbyist character of beekeeping and never affect honey production. We amended our project design following their suggestions. In addition, we collaborated with Design Academy Eindhoven to design a tea bag like product that beekeeper can easily apply.