Difference between revisions of "Team:Wageningen UR/Notebook"
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| + | <h3 text-align="center">Find the documentation of our research here.</h3> | ||
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| − | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/ProteinEngineering" style="border:5px solid # | + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/ProteinEngineering" style="border:5px solid #fff372"> <!--Change box color here--> |
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| − | <img src="https://static.igem.org/mediawiki/2016/ | + | <img src="https://static.igem.org/mediawiki/2016/d/da/T--Wageningen_UR--engibutton.jpg"/> |
<h3 style="color:#6d5d31">Protein Engineering</h3> <!--Change text color here--> | <h3 style="color:#6d5d31">Protein Engineering</h3> <!--Change text color here--> | ||
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| − | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/InVitroAssay" style="border:5px solid # | + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/InVitroAssay" style="border:5px solid #fff372"> <!--Change box color here--> |
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| − | <img src="https://static.igem.org/mediawiki/2016/ | + | <img src="https://static.igem.org/mediawiki/2016/5/55/T--Wageningen_UR--vesibutton.jpg"/> |
<h3 style="color:#6d5d31"><i>In Vitro</i> Assay</h3> <!--Change text color here--> | <h3 style="color:#6d5d31"><i>In Vitro</i> Assay</h3> <!--Change text color here--> | ||
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| − | <a href=https://2016.igem.org/Team:Wageningen_UR/Notebook/VarroaIsolates style="border:5px solid # | + | <a href=https://2016.igem.org/Team:Wageningen_UR/Notebook/VarroaIsolates style="border:5px solid #fff372"> <!--Change box color here--> |
<div class="square-menu-header"> | <div class="square-menu-header"> | ||
| − | <img src="https://static.igem.org/mediawiki/2016/ | + | <img src="https://static.igem.org/mediawiki/2016/a/a3/T--Wageningen_UR--mitebutton.jpg"/> |
<h3 style="color:#6d5d31"><i>Varroa</i> isolates</h3> <!--Change text color here--> | <h3 style="color:#6d5d31"><i>Varroa</i> isolates</h3> <!--Change text color here--> | ||
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| − | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/Riboswitch" style="border:5px solid # | + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/Riboswitch" style="border:5px solid #fff372"> <!--Change box color here--> |
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| − | <img src="https://static.igem.org/mediawiki/2016/ | + | <img src="https://static.igem.org/mediawiki/2016/f/f4/T--Wageningen_UR--ribobutton.jpg"/> |
<h3 style="color:#6d5d31">Riboswitches</h3> <!--Change text color here--> | <h3 style="color:#6d5d31">Riboswitches</h3> <!--Change text color here--> | ||
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| − | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/QuorumSensing" style="border:5px solid # | + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/QuorumSensing" style="border:5px solid #fff372"> <!--Change box color here--> |
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| − | <img src="https://static.igem.org/mediawiki/2016/ | + | <img src="https://static.igem.org/mediawiki/2016/b/b1/T--Wageningen_UR--quorumbutton.jpg"/> |
| − | <h3 style="color:#6d5d31"> | + | <h3 style="color:#6d5d31">Population Dynamics</h3 > <!--Change text color here--> |
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<p>A quorum sensing system was characterized to regulate toxin production in BeeT.</p> | <p>A quorum sensing system was characterized to regulate toxin production in BeeT.</p> | ||
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| − | <a href=" | + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/OptogeneticKillSwitch" style="border:5px solid #fff372"> <!--Change box color here--> |
<div class="square-menu-header"> | <div class="square-menu-header"> | ||
| − | <img src="https://static.igem.org/mediawiki/2016/ | + | <img src="https://static.igem.org/mediawiki/2016/6/61/T--Wageningen_UR--lightbutton.jpg"/> |
| − | <h3 style="color:#6d5d31"> | + | <h3 style="color:#6d5d31; font-size:18px;">Optogenetic Kill Switch</h3> <!--Change text color here--> |
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<p>The light-induced kill switch was one of our biocontainment methods.</p> | <p>The light-induced kill switch was one of our biocontainment methods.</p> | ||
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| − | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/Chitinases" style="border:5px solid # | + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/Chitinases" style="border:5px solid #fff372"> <!--Change box color here--> |
<div class="square-menu-header"> | <div class="square-menu-header"> | ||
| − | <img src="https://static.igem.org/mediawiki/2016/ | + | <img src="https://static.igem.org/mediawiki/2016/a/a3/T--Wageningen_UR--mitebutton.jpg"/> |
<h3 style="color:#6d5d31">Chitinases</h3> <!--Change text color here--> | <h3 style="color:#6d5d31">Chitinases</h3> <!--Change text color here--> | ||
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| − | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/Cas9" style="border:5px solid # | + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/Cas9" style="border:5px solid #fff372"> <!--Change box color here--> |
<div class="square-menu-header"> | <div class="square-menu-header"> | ||
| − | <img src="https://static.igem.org/mediawiki/2016/7/ | + | <img src="https://static.igem.org/mediawiki/2016/7/76/T--Wageningen_UR--casbutton.jpg"/> |
| − | <h3 style="color:#6d5d31">Cas9-based Kill Switch</h3> <!--Change text color here--> | + | <h3 style="color:#6d5d31; font-size:18px;">Cas9-based Kill Switch</h3> <!--Change text color here--> |
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<p>The Cas9-based kill switch was one of our biocontainment methods.</p> | <p>The Cas9-based kill switch was one of our biocontainment methods.</p> | ||
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| − | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/ToxinScanner" style="border:5px solid # | + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/ToxinScanner" style="border:5px solid #fff372"> <!--Change box color here--> |
<div class="square-menu-header"> | <div class="square-menu-header"> | ||
| − | <img src="https://static.igem.org/mediawiki/2016/ | + | <img src="https://static.igem.org/mediawiki/2016/a/a3/T--Wageningen_UR--mitebutton.jpg"/> |
<h3 style="color:#6d5d31">Toxin Scanner</h3> <!--Change text color here--> | <h3 style="color:#6d5d31">Toxin Scanner</h3> <!--Change text color here--> | ||
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| − | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/ | + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/ecoli_survival" style="border:5px solid #fff372"> <!--Change box color here--> |
<div class="square-menu-header"> | <div class="square-menu-header"> | ||
| − | <img src="https://static.igem.org/mediawiki/2016/ | + | <img src="https://static.igem.org/mediawiki/2016/5/53/T--Wageningen_UR--metabutton.jpg"/> |
| − | <h3 style="color:#6d5d31"> | + | <h3 style="color:#6d5d31;"><i>E. coli</i> survival</h3> <!--Change text color here--> |
</div> | </div> | ||
| − | <p> | + | <p>Following the results of the Metabolic Model, we needed more data.</p> |
</a> | </a> | ||
</li> | </li> | ||
| + | <li> | ||
| + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/toggleswitch" style="border:5px solid #fff372"> <!--Change box color here--> | ||
| + | <div class="square-menu-header"> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/9/96/T--Wageningen_UR--togglebutton.jpg"/> | ||
| + | <h3 style="color:#6d5d31">Toggle Switch</h3> <!--Change text color here--> | ||
| + | </div> | ||
| + | <p font style="font-size:15px; font-family: snap,arial, sans-serif";> We designed a toggle switch that could coordinate mites sensing, BT's toxin production and light sensing with fast responsiveness.</p> | ||
| + | </a> | ||
| + | </li> | ||
| + | <li> | ||
| + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/Outreach" style="border:5px solid #fff372"> <!--Change box color here--> | ||
| + | <div class="square-menu-header"> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/b/bf/T--Wageningen_UR--outreachbutton.jpg"/> | ||
| + | <h3 style="color:#6d5d31">Human Outreach</h3> <!--Change text color here--> | ||
| + | </div> | ||
| + | <p>We implemented our outreach into our research as much as we could. Find the details here!</p> | ||
| + | </a> | ||
| + | </li> | ||
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| + | <li> | ||
| + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/beehave" style="border:5px solid #fff372"> <!--Change box color here--> | ||
| + | <div class="square-menu-header"> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/9/96/T--Wageningen_UR--togglebutton.jpg"/> | ||
| + | <h3 style="color:#6d5d31">BEEHAVE</h3> <!--Change text color here--> | ||
| + | </div> | ||
| + | <p font style="font-size:18px; font-family: snap,arial, sans-serif";> We adapted the BEEHAVE model to include BeeT. </p> | ||
| + | </a> | ||
| + | </li> | ||
| + | </li> | ||
| + | <li> | ||
| + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/popmodel" style="border:5px solid #fff372"> <!--Change box color here--> | ||
| + | <div class="square-menu-header"> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/b/b1/T--Wageningen_UR--quorumbutton.jpg"/> | ||
| + | <h3 style="color:#6d5d31">Population Model</h3> <!--Change text color here--> | ||
| + | </div> | ||
| + | <p> Modelling of the population dynamics regulation system.</i>. </p> | ||
| + | </a> | ||
| + | </li> | ||
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| + | <li> | ||
| + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/OptogeneticKillSwitchModel" style="border:5px solid #fff372"> <!--Change box color here--> | ||
| + | <div class="square-menu-header"> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/6/61/T--Wageningen_UR--lightbutton.jpg"/> | ||
| + | <h3 style="color:#6d5d31; font-size:18px;">Kill Switch Model</h3> <!--Change text color here--> | ||
| + | </div> | ||
| + | <p>The optogenetic kill switch was modeled with ODEs to understand the system in more detail.</p> | ||
| + | </a> | ||
| + | </li> | ||
<li> | <li> | ||
| − | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/ | + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/MetabolicModelling" style="border:5px solid #fff372"> <!--Change box color here--> |
<div class="square-menu-header"> | <div class="square-menu-header"> | ||
| − | <img src="https://static.igem.org/mediawiki/2016/ | + | <img src="https://static.igem.org/mediawiki/2016/5/53/T--Wageningen_UR--metabutton.jpg"/> |
| − | <h3 style="color:#6d5d31"> | + | <h3 style="color:#6d5d31">Metabolic Modelling</h3> <!--Change text color here--> |
</div> | </div> | ||
| − | <p> | + | <p>We wanted to use a metabolic model to see the effect of our BeeT distribution method.</p> |
</a> | </a> | ||
</li> | </li> | ||
Latest revision as of 17:32, 19 October 2016
Find the documentation of our research here.
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Protein Engineering
We wanted to engineer Cry3Aa to become toxic to Varroa destructor.
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In Vitro Assay
To provide an alternative for tests on live Varroa mites, we designed an assay that uses brush border membrane vesicles.
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Varroa isolates
We tried to find our own Varroa entomopathogen.
-
Riboswitches
To detect the presence of Varroa mites in the beehive, we made a B12 and a guanine riboswitch.
-
Population Dynamics
A quorum sensing system was characterized to regulate toxin production in BeeT.
-
Optogenetic Kill Switch
The light-induced kill switch was one of our biocontainment methods.
-
Chitinases
As a back-up, we wanted to use chitinases which are more harmful to Varroa destructor than honeybees.
-
Cas9-based Kill Switch
The Cas9-based kill switch was one of our biocontainment methods.
-
Toxin Scanner
We made a software tool for finding new Cry proteins toxic to Varroa destructor.
-
E. coli survival
Following the results of the Metabolic Model, we needed more data.
-
Toggle Switch
We designed a toggle switch that could coordinate mites sensing, BT's toxin production and light sensing with fast responsiveness.
-
Human Outreach
We implemented our outreach into our research as much as we could. Find the details here!
-
BEEHAVE
We adapted the BEEHAVE model to include BeeT.
-
Population Model
Modelling of the population dynamics regulation system..
-
Kill Switch Model
The optogenetic kill switch was modeled with ODEs to understand the system in more detail.
-
Metabolic Modelling
We wanted to use a metabolic model to see the effect of our BeeT distribution method.
