Difference between revisions of "Team:NYMU-Taipei/Notebook-Lab Book-pGFP C120 RFP"

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<style>
 
<style>
 +
@media screen and (min-width: 300px) and (max-width: 1000px){
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.expand{ /*header of 2nd demo*/
 
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   padding: 8px;
 
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 +
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 +
  cursor: pointer;
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<body>
 
<body>
 +
 +
    <div class="prototypeprototypesp">
 +
        <div class="imageimage00">
 +
            <img src="https://static.igem.org/mediawiki/2016/1/1c/T--NYMU-Taipei--%E6%96%B0pGFP_C120_RFP.png" width="100%" height="100%" />
 +
        </div>
 +
    </div>
 +
 +
    <div class="prototypeprototype">
 +
        <div class="imageimage00">
 +
            <img src="https://static.igem.org/mediawiki/2016/1/1c/T--NYMU-Taipei--%E6%96%B0pGFP_C120_RFP.png" width="100%" height="100%" />
 +
        </div>
 +
    </div>
  
 
<div id="wrap">
 
<div id="wrap">
−
<div class="imageimage1">
+
<div class="fund">
−
<div class="wordword1">
+
<li style="list-style-type:disc;"><h3 style="margin-top:10px; margin-bottom:0px; line-height: 24px;"><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-pBARGPE1">pBARGPE1</a></h3></li>
  
−
<h1>TITLE</h1><hr />
+
<li style="list-style-type:disc;"><h3 style="margin-top:10px; margin-bottom:0px; line-height: 24px;"><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-GFP">GFP</a></h3></li>
−
<p>Besides the fungal killing switch and the functional prototype that help reduce concerns over GMO</p>
+
  
−
</div></div></div>
+
<li style="list-style-type:disc;"><h3 style="margin-top:10px; margin-bottom:0px; line-height: 24px;"><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-TtrpC">TtrpC</a></h3></li>
 +
 
 +
<li style="list-style-type:disc;"><h3 style="margin-top:10px; margin-bottom:0px; line-height: 24px;"><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-C120_empty">C120_empty</a></h3></li>
 +
 
 +
<li style="list-style-type:disc;"><h3 style="margin-top:10px; margin-bottom:0px; line-height: 24px;"><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-minimal-PgpdA">minimal-PgpdA</a></h3></li>
 +
 
 +
<li style="list-style-type:disc;"><h3 style="margin-top:10px; margin-bottom:0px; line-height: 24px;"><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-RFP">RFP</a></h3></li>
 +
 
 +
</div>
 +
<br />
 +
</div>
  
 
<div class="expand">
 
<div class="expand">
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style="color: #666666 !important; text-shadow: 0px 0px 1px #000000 !important;  /*margin: 12px !important;*/  font-size: 18px !important;  text-decoration: none;">[Expand all]</a></p></center></div>
 
style="color: #666666 !important; text-shadow: 0px 0px 1px #000000 !important;  /*margin: 12px !important;*/  font-size: 18px !important;  text-decoration: none;">[Expand all]</a></p></center></div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">9/23</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">GFP_TtrpC ligation PCR</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">Week 2: (1-7 July)</div>
+
<div class="technology">9/24</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>The ordered primers were taken this week.</p>
+
<li><p style="font-size: 18px;">GFP_TtrpC ligation PCR</p></li>
−
<p>We would produce our biobricks, but the result was not like we expected after electrophoresis. Then we made colony PCR  and again do it for SOD and GPX. After this, we did digestion and also pTRE vector .</p>
+
<li><p style="font-size: 18px;">GFP_TtrpC ligation PCR (We confirmed junction primer was wrong)</p></li>
−
<img src="https://static.igem.org/mediawiki/2014/7/7e/ATOMS-Notebook1.tif.png">
+
−
<img src="https://static.igem.org/mediawiki/2014/1/17/ATOMS-Notebook2.png">
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 1); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 1); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">9/30</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">Received the junction primer of GFP-TtrpC</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">GFP_TtrpC ran ligation PCR</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 2); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 2); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">10/1</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">GFP_TtrpC ran ligation PCR</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 3); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 3); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">10/2</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">GFP_TtrpC digested for ligation with pBARGPE1</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">Transformed pBAR_GFP_TtrpC into competent cells</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 4); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 4); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">10/3</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pBAR_GFP_TtrpC containing cultures used in 2 in 1</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pExtracted plasmid pBAR_GFP_TtrpC</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pBAR_GFP_TtrpC digestion check(failure)</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pBAR_GFP_TtrpC PCR check(failure)</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">Transformed pBAR_GFP_TtrpC into competent cells</p></li>
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">10/4</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pBAR_GFP_TtrpC containing cultures used in 2 in 1</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pBAR_GFP_TtrpC plasmid extraction</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pBAR_GFP_TtrpC digestion check</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 6); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 6); return false">[Collapse]</a></p>
−
</div>
 
−
 
−
<div class="technology">transformation efficiency </div>
 
−
<div class="thelanguage">
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 7); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">transformation efficiency </div>
 
−
<div class="thelanguage">
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 8); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">transformation efficiency </div>
 
−
<div class="thelanguage">
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 9); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">transformation efficiency </div>
 
−
<div class="thelanguage">
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 10); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">transformation efficiency </div>
 
−
<div class="thelanguage">
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 11); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">transformation efficiency </div>
 
−
<div class="thelanguage">
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 12); return false">[Collapse]</a></p>
 
 
</div>
 
</div>
  

Latest revision as of 21:43, 19 October 2016

[Collapse all] | [Expand all]

9/23

  • GFP_TtrpC ligation PCR

    • [Collapse]

    9/24

  • GFP_TtrpC ligation PCR

  • GFP_TtrpC ligation PCR (We confirmed junction primer was wrong)

    • [Collapse]

    9/30

  • Received the junction primer of GFP-TtrpC

  • GFP_TtrpC ran ligation PCR

    • [Collapse]

    10/1

  • GFP_TtrpC ran ligation PCR

    • [Collapse]

    10/2

  • GFP_TtrpC digested for ligation with pBARGPE1

  • Transformed pBAR_GFP_TtrpC into competent cells

    • [Collapse]

    10/3

  • pBAR_GFP_TtrpC containing cultures used in 2 in 1

  • pExtracted plasmid pBAR_GFP_TtrpC

  • pBAR_GFP_TtrpC digestion check(failure)

  • pBAR_GFP_TtrpC PCR check(failure)

  • Transformed pBAR_GFP_TtrpC into competent cells

    • [Collapse]

    10/4

  • pBAR_GFP_TtrpC containing cultures used in 2 in 1

  • pBAR_GFP_TtrpC plasmid extraction

  • pBAR_GFP_TtrpC digestion check

    • [Collapse]