Difference between revisions of "Team:Glasgow"

 
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<p class="copy">Vitamin deficiencies are a major problem across the globe, especially in developing countries. Hypovitaminosis A (deficiency in Vitamin A) is the primary cause of preventable blindness in children, whereas folate deficiency is linked to an increased rate of spina bifida.</p>
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<p class="copy">Vitamin deficiencies are a major problem across the globe, especially in low-resource settings. Vitamin A deficiency (hypovitaminosis A) is the primary cause of preventable blindness in children. We hoped to target this ailment by manipulating yogurt bacteria to produce beta-carotene - a precursor that can be converted into vitamin A by the body.
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<p class="copy">We hope to target these ailments by manipulating yogurt bacteria to produce beta-carotene and produce higher levels of folate. Our final goal is to introduce three genes in the beta-carotene synthesis pathway and upregulate the production of folate in a strain of lactic acid bacteria e.g. Streptococcus thermophilus. The yogurt produced from the bacteria would be rich in both beta-carotene (which is converted by the body into Vitamin A) and folate.</p>
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                Production of beta-carotene coinciding with the conversion of milk to yogurt would result in an enriched dairy product. Considering dried bacteria can be easily packaged and distributed and milk is readily available in many of the areas that have high incidence of hypovitaminosis A, we think this could be an ideal solution.</p>
 
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<p class="copy">Alongside this, we also aim to characterise lactic acid bacteria in various ways in order to increase understanding and ability to work with these potentially useful organisms.</p>
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<p class="copy">We successfully introduced four genes in the beta-carotene synthesis pathway into E. coli and designed a shuttle vector for these genes to be transferred into the yogurt bacteria S. thermophilus. In order to improve understanding of this bacteria, we developed S. thermophilus as a chassis and explored its behaviour using transcriptomics. We hope this will encourage people to consider using S. thermophilus and yogurt as a vector for nutrition in the future.</p>
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<p class="copy">Additionally, we aim to make an accessible solar water bath incubator that could be used in developing countries to pasteurise milk and make yogurt.</p>
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<p class="copy">Considering the popularity of yogurt in many low-resource settings, we aimed to enhance manufacturing ability. To this end, we also designed an accessible solar concentrator to assist with pasteurization of milk and highly efficient production of yogurt.</p>
 
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Engineering <span class="i">E. Coli</span> to produce beta-carotene.
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Engineering <span class="i">E. Coli</span> to produce beta-carotene
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Creating a genetic toolkit for working with <span class="i">S. thermophilus</span>
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Engineering a mechanism of inactivation to improve public perception
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Designing a solar concentrator for accessible yogurt production
 
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Latest revision as of 23:09, 19 October 2016

Glasgow iGEM 2016
Enriching yoghurt with beta-carotene
Using synthetic biology to tackle vitamin A deficiency
Project Description

Vitamin deficiencies are a major problem across the globe, especially in low-resource settings. Vitamin A deficiency (hypovitaminosis A) is the primary cause of preventable blindness in children. We hoped to target this ailment by manipulating yogurt bacteria to produce beta-carotene - a precursor that can be converted into vitamin A by the body. Production of beta-carotene coinciding with the conversion of milk to yogurt would result in an enriched dairy product. Considering dried bacteria can be easily packaged and distributed and milk is readily available in many of the areas that have high incidence of hypovitaminosis A, we think this could be an ideal solution.

We successfully introduced four genes in the beta-carotene synthesis pathway into E. coli and designed a shuttle vector for these genes to be transferred into the yogurt bacteria S. thermophilus. In order to improve understanding of this bacteria, we developed S. thermophilus as a chassis and explored its behaviour using transcriptomics. We hope this will encourage people to consider using S. thermophilus and yogurt as a vector for nutrition in the future.

Considering the popularity of yogurt in many low-resource settings, we aimed to enhance manufacturing ability. To this end, we also designed an accessible solar concentrator to assist with pasteurization of milk and highly efficient production of yogurt.

Engineering E. Coli to produce beta-carotene
Creating a genetic toolkit for working with S. thermophilus
Engineering a mechanism of inactivation to improve public perception
Designing a solar concentrator for accessible yogurt production