Difference between revisions of "Team:Michigan/Medals"

 
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           <h1 style="text-align:center; font-size: 60px; color:black;"><font face= "Poiret One">Bronze Requirements</h1>
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           <h1 style="text-align:center; font-size: 60px; text-color:black;"><font face= "Poiret One">Bronze Requirements</h1>
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           <p style="text-align:center; font-size:20px;"><font face="verdana"><ol class="content1 contentMedalRequirements">
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           <p style="text-align:left; font-size:20px;"><font face="verdana"><b>3. Create a page on your team wiki with clear attribution of each aspect of your project.</b><br>
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      <li>Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected. Document the characterization of this part in the Main Page section of the Registry entry for that Part/Device. This working part must be different from the part you documented in Bronze medal criterion #6.
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The contributions each team member made to this year's final project are detailed on <a href="https://2016.igem.org/Team:Michigan/Attributions">this page</a>.<br><br>
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<b>4. Document at least one new standard BioBrick Part or Device central to your project and submit this part to the iGEM Registry. You may also document a new application of a BioBrick part from a previous iGEM year, adding that documentation to the part main page.</b><br>
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We verified and classified our parts/devices by means of flow cytometry analysis. We validated the expression strength of three different alpha-factor responsive promoters by creating circuits in which fluorescent reporters were being driven by the promoter. We induced these promoters with purified alpha factor and measured them with flow cytometry to determine the expression strength of each promoter. The promoters characterized were pFig2c (<a style="color:#44C2CC" href="http://parts.igem.org/Part:BBa_K1829002" target="_blank">BBa_K1829002</a>), pBar1 (<a style="color:#44C2CC" href="http://parts.igem.org/Part:BBa_K1829001" target="_blank">BBa_K1829001</a>), and pAga1 (<a style="color:#44C2CC" href="http://parts.igem.org/Part:BBa_K1829005" target="_blank">BBa_K1829005</a>); all are endogenous yeast promoters that participate in the mating response pathway.
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<a href="http://parts.igem.org/Part:BBa_E0033">Part BBa E0033</a> compliments our new part, <a href="http://parts.igem.org/Part:BBa_K2105000"> BBa K2105000</a>, both of which are explained on <a href="https://2016.igem.org/Team:Michigan/Parts">this page</a>.<br></font><br></p>
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We additionally submitted the yeast protease Bar1 (<a style="color:#44C2CC" href="http://parts.igem.org/Part:BBa_K1829000" target="_blank">BBa_K1829000</a>) which cleaves alpha factor, and made silent mutations to make it BioBrick compatible. We characterized the effect of adding Bar1 to our communication circuit with alpha factor, showing it effectively degrades alpha factor and reduces circuit sensitivity.
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Our parts were integrated within the pSB1C3 plasmid. All parts delivered are risk group one organisms and do not violate any safety standards. Specific part details can be found on our iGEM parts registry page and our <a style="color:#44C2CC" href="https://2015.igem.org/Team:UCSF/Parts" target="_blank">Parts Page.</a>
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      <li>Submit this new part to the iGEM Parts Registry.
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The parts have been submitted!
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      <li>iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, and intellectual property rights. We refer to these activities as Human Practices in iGEM. Demonstrate how your team has identified, investigated and addressed one or more of these issues in the context of your project.
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This year, in addition to addressing cellular communication, we wanted to explore other issues of communication, specifically between the scientific community and the general public. Despite the breadth of iGEM and the open-source nature of its data reporting, the wikis and science content still remain very complex and not easily accessed by members of the general public.
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We addressed this with three ambitious projects:
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    <li style="color:white">We utilized social media, such as Twitter and Youtube, as a way of conveying scientific research to the general public through a <b>video series on synthetic biology and research</b>.
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    <li style="color:white">We contributed to the planning of a <b>Synthetic Biology conference</b> -- the Sierra Systems and SynBio Symposium -- which highlights undergraduate research in synbio. As a part of that conference, <b>we organized an iGEM Meetup</b> and coordinated 4 other teams attending as well as science presentations and social activities.
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    <li style="color:white">We started a <b>new initiative called Wiki Flicks</b>, where we encouraged the other iGEM teams, including ourselves, to create a short, interesting video about their project that is easy for ANYONE understand.
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           <h1 style="text-align:center; font-size: 60px;"><font face= "Poiret One">Silver Requirements</h1>
 
           <h1 style="text-align:center; font-size: 60px;"><font face= "Poiret One">Silver Requirements</h1>
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         <p style="text-align:center; font-size:20px;"><font face="verdana"><br>Any project must have solid roots and be viable in order to be able to implement it in real life. To achieve this, our team was sure to meet with health professionals both involved in the field of tuberculosis and/or synthetic biology, which helped shape the direction and concept of our project from its scientific concept in the wet lab all the way to its possible implementation as a market product. Our team consulted with the president of the Relief for Africa foundation, Rama Kannenje, who indicated the prevalent need for point-of-care diagnostics. Additionally, experts like Grace Hsia, the CEO of Warmilu, brought to our attention the widespread poverty in countries where tuberculosis is endemic. During our participation in the Ann Arbor Health Hacks we also met with many health professionals who contributed ideas that improved our project.<br><br>
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         <p style="text-align:left; font-size:20px;"><font face="verdana"><br><b>1. Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.</b><br>
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You can read in more detail about how we implemented professional’s opinions into our project in our <a href="https://2016.igem.org/Team:Michigan/Integrated_Practices">integrated practices tab</a>.</font></p>
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We submitted <a href="http://parts.igem.org/Part:BBa_K2105000">BBa_K2105000</a> as a brand new part. It creates an incomplete molecule as a step in our project, and is further explained <a href="https://2016.igem.org/Team:Michigan/Parts">here</a>.<br><br>
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<b>2. Convince the judges you have helped any registered iGEM team from high school, a different track, another university, or another institution in a significant way.</b><br>
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We worked with both <a href="https://2016.igem.org/Team:Michigan/Collaborations">Team Sydney Australia and Team Michigan Software</a>, but most notably created a <a href="https://2016.igem.org/Team:Michigan/factsheet"> bench-to-bedside guide</a> that can be used by other diagnostic track teams.<br><br>
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<b>3. Demonstrate how your team has identified, investigated, and addressed ethics, sustainability, social justice, safety, security, and intellectual property rights.</b><br>
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A lot of effort went into addressing several <a href="https://2016.igem.org/Team:Michigan/Engagement">human practice</a> components, including making <a href="http://www.synbiofactcheck.com/">website</a> that discusses ethical concerns and public education. We also proposed a <a href="https://2016.igem.org/Team:Michigan/Safety">safety system</a> that could be applied in future synthetic biology projects.<br><br></font></p>
 
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           <h1 style="text-align:center; font-size: 60px;"><font face= "Poiret One">Gold Requirements</h1>
 
           <h1 style="text-align:center; font-size: 60px;"><font face= "Poiret One">Gold Requirements</h1>
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           <p style="text-align:center; font-size:20px;"><font face="verdana"><br> Starting with the simplest of safety principles in the lab and moving up, the team was able to assure a safe design phase as well as provide an environmentally safe final project. Conventional laboratory safety procedures as mandated by the University of Michigan’s department of safety and common good practice were followed. When considering the hazards posed to organisms by the project, E. coli was the only organism used. Even then it was only included as a cloning and expression agent, and never left the wet lab. Also included in our design is a theoretical bio-part that would ensure that the project’s DNA cannot spread into environmental organisms using DNase. This part could be applied broadly in future synthetic biology projects as a self-erasing kill switch that cannot damage the protected DNA of other organisms.<br><br>
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           <p style="text-align:left; font-size:20px;"><font face="verdana"><br><b>1. Expand on your silver medal activity by demonstrating how you have integrated the investigated issues into the design and/or execution of your project.</b><br>
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You can read in more detail about safety issues regarding our project in our <a href="https://2016.igem.org/Team:Michigan/Safety">safety tab.</a></font><br><hr></p>
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We talked to experts in <a href="https://2016.igem.org/Team:Michigan/Integrated_Practices">public health and medicine</a> about the applications of our project. This led to a handful of changes in our design as well as our approach to the project as a potentially scaling project.<br><br>
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<b>3. Demonstrate a functional proof of concept of your project.</b><br>
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For proof of concept, please visit the <a href="http://parts.igem.org/Part:BBa_K2105000">parts registry page for BBa_K2105000</a>.<br></font><br></p>
 
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Latest revision as of 01:41, 20 October 2016

Home Project Modeling Team BioBrick Human Practices Safety Awards

Medals

Bronze Requirements

3. Create a page on your team wiki with clear attribution of each aspect of your project.
The contributions each team member made to this year's final project are detailed on this page.

4. Document at least one new standard BioBrick Part or Device central to your project and submit this part to the iGEM Registry. You may also document a new application of a BioBrick part from a previous iGEM year, adding that documentation to the part main page.
Part BBa E0033 compliments our new part, BBa K2105000, both of which are explained on this page.

Silver Requirements


1. Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.
We submitted BBa_K2105000 as a brand new part. It creates an incomplete molecule as a step in our project, and is further explained here.

2. Convince the judges you have helped any registered iGEM team from high school, a different track, another university, or another institution in a significant way.
We worked with both Team Sydney Australia and Team Michigan Software, but most notably created a bench-to-bedside guide that can be used by other diagnostic track teams.

3. Demonstrate how your team has identified, investigated, and addressed ethics, sustainability, social justice, safety, security, and intellectual property rights.
A lot of effort went into addressing several human practice components, including making website that discusses ethical concerns and public education. We also proposed a safety system that could be applied in future synthetic biology projects.

Gold Requirements


1. Expand on your silver medal activity by demonstrating how you have integrated the investigated issues into the design and/or execution of your project.
We talked to experts in public health and medicine about the applications of our project. This led to a handful of changes in our design as well as our approach to the project as a potentially scaling project.

3. Demonstrate a functional proof of concept of your project.
For proof of concept, please visit the parts registry page for BBa_K2105000.