Difference between revisions of "Team:Lethbridge"
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<p class="grey-text text-lighten-4">Recognizing the need for high-throughput and inclusive pathogen detection methods, we developed a bacterial detection platform, utilizing the MinION - a novel NGS sequencing technology from Oxford Nanopore Technologies.</p> | <p class="grey-text text-lighten-4">Recognizing the need for high-throughput and inclusive pathogen detection methods, we developed a bacterial detection platform, utilizing the MinION - a novel NGS sequencing technology from Oxford Nanopore Technologies.</p> | ||
| − | <center><img src="https://static.igem.org/mediawiki/2016/5/5c/T--Lethbridge--NanoporeSeq.jpg" width= | + | <center><img src="https://static.igem.org/mediawiki/2016/5/5c/T--Lethbridge--NanoporeSeq.jpg" width=50%> |
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<p class="grey-text text-lighten-4">The MinION Next Geneneration Sequencing Platform</p></center> | <p class="grey-text text-lighten-4">The MinION Next Geneneration Sequencing Platform</p></center> | ||
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<p class="grey-text text-lighten-4">In collaboration with our local EMS department, we devised a sampling strategy and DNA extraction procedure that is compatible with the MinION sequencing technology.</p> | <p class="grey-text text-lighten-4">In collaboration with our local EMS department, we devised a sampling strategy and DNA extraction procedure that is compatible with the MinION sequencing technology.</p> | ||
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<p class="grey-text text-lighten-4">Sampling locations for the EMS Microbiome Study</p></center> | <p class="grey-text text-lighten-4">Sampling locations for the EMS Microbiome Study</p></center> | ||
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<h3 class="white-text thin">Single Domain Antibody Development System</h3> | <h3 class="white-text thin">Single Domain Antibody Development System</h3> | ||
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| + | <p class="grey-text text-lighten-4">Engineered Bacterial-two-hybrid Antibody Selection System</p></center> | ||
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Revision as of 02:37, 20 October 2016
Nanoresponder - Call to Action!
With ever increasing threats from emergent pathogens and multiple resistant bacteria, hospitals are cracking down with more rigorous monitoring and decontamination procedures to prevent pathogen spread.
Emergency Medical Service (EMS) vehicles and workers are often the first point of patient care, however, little is known about the importance of EMS vehicles for the transmission of pathogens, and detection methods lag behind those of hospitals.
We are Nanoresponder! A team dedicated to the advancement of pathogen detection and monitoring in EMS vehicles. We have taken a dual approach to pathogen detection: (1) Nanopore Next Generation Sequencing (NGS) of DNA samples from EMS vehicles; and (2) A rapid single-domain antibody detection system to generate antibodies that may be implemented in simple and reliable testing kits for fast, on-site pathogen detection.
EMS Vehicle Microbiome Investigation
Upon conducting an in-depth review of literature surrounding EMS vehicle pathogen detection and interviews with EMS officials, we discovered that no published study utilizes a Next Generation Sequencing approach to pathogen detection in EMS vehicles and instead solely cell culturing techniques were used. This is surprising considering that many microbes are unculturable in a laboratory setting and would otherwise escape detection. Moreover, cell culturing techniques may requires many days before test results are available.
Recognizing the need for high-throughput and inclusive pathogen detection methods, we developed a bacterial detection platform, utilizing the MinION - a novel NGS sequencing technology from Oxford Nanopore Technologies.
The MinION Next Geneneration Sequencing Platform
In collaboration with our local EMS department, we devised a sampling strategy and DNA extraction procedure that is compatible with the MinION sequencing technology.
Sampling locations for the EMS Microbiome Study
Single Domain Antibody Development System
Engineered Bacterial-two-hybrid Antibody Selection System
